Circular RNA hsa_Circ_101141 as a Competing Endogenous RNA Facilitates Tumorigenesis of Hepatocellular Carcinoma by Regulating miR-1297/ROCK1 Pathway
As a novel class of noncoding RNAs, circular RNAs (circRNAs) have been recently reported to be involved in cell development and function. However, the functional role of circRNAs in hepatocellular carcinoma (HCC) remains unclear. In the present study, we found that the expression of human circ_10114...
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Online Access: | https://doi.org/10.1177/0963689720948016 |
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doaj-ae22cd0866454bca97e0bae0bd5402c42020-11-25T03:35:15ZengSAGE PublishingCell Transplantation1555-38922020-08-012910.1177/0963689720948016Circular RNA hsa_Circ_101141 as a Competing Endogenous RNA Facilitates Tumorigenesis of Hepatocellular Carcinoma by Regulating miR-1297/ROCK1 PathwayTao Zhang0Lijuan Zhang1Dan Han2Kebinur Tursun3Xiaobo Lu4 Department of Infectious Disease Center, , Urumqi City, Xinjiang Uygur Autonomous Region, China Department of Infectious Disease Center, , Urumqi City, Xinjiang Uygur Autonomous Region, China Department of Infectious Disease Center, , Urumqi City, Xinjiang Uygur Autonomous Region, China Department of Infectious Disease Center, , Urumqi City, Xinjiang Uygur Autonomous Region, China Department of Infectious Disease Center, , Urumqi City, Xinjiang Uygur Autonomous Region, ChinaAs a novel class of noncoding RNAs, circular RNAs (circRNAs) have been recently reported to be involved in cell development and function. However, the functional role of circRNAs in hepatocellular carcinoma (HCC) remains unclear. In the present study, we found that the expression of human circ_101141 was upregulated in HCC tissues and cells. In addition, downregulation of circ_101141 dramatically inhibited cell proliferation, migration, and invasion in HCC cells. In addition, by using the bioinformatics tools, the potential target of circ_101141 was predicted. Mechanistic investigations indicated that circ_101141 acted as a miR-1297 “sponge”; meanwhile, Rho-associated, coiled-coil-containing protein kinase 1 (ROCK1) was a direct target of miR-1297. Further experiments demonstrated that circ_101141 contributed to the progression of HCC by acting as competing endogenous RNA (ceRNA) of miR-1297 to regulate ROCK1 expression. Furthermore, knockdown of circ_101141 attenuated HCC tumorigenesis in vivo. Taken together, these findings indicated that circRNA circ_101141 acted as a ceRNA to facilitate tumorigenesis of HCC by regulating miR-1297/ROCK1 pathway.https://doi.org/10.1177/0963689720948016 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tao Zhang Lijuan Zhang Dan Han Kebinur Tursun Xiaobo Lu |
spellingShingle |
Tao Zhang Lijuan Zhang Dan Han Kebinur Tursun Xiaobo Lu Circular RNA hsa_Circ_101141 as a Competing Endogenous RNA Facilitates Tumorigenesis of Hepatocellular Carcinoma by Regulating miR-1297/ROCK1 Pathway Cell Transplantation |
author_facet |
Tao Zhang Lijuan Zhang Dan Han Kebinur Tursun Xiaobo Lu |
author_sort |
Tao Zhang |
title |
Circular RNA hsa_Circ_101141 as a Competing Endogenous RNA Facilitates Tumorigenesis of Hepatocellular Carcinoma by Regulating miR-1297/ROCK1 Pathway |
title_short |
Circular RNA hsa_Circ_101141 as a Competing Endogenous RNA Facilitates Tumorigenesis of Hepatocellular Carcinoma by Regulating miR-1297/ROCK1 Pathway |
title_full |
Circular RNA hsa_Circ_101141 as a Competing Endogenous RNA Facilitates Tumorigenesis of Hepatocellular Carcinoma by Regulating miR-1297/ROCK1 Pathway |
title_fullStr |
Circular RNA hsa_Circ_101141 as a Competing Endogenous RNA Facilitates Tumorigenesis of Hepatocellular Carcinoma by Regulating miR-1297/ROCK1 Pathway |
title_full_unstemmed |
Circular RNA hsa_Circ_101141 as a Competing Endogenous RNA Facilitates Tumorigenesis of Hepatocellular Carcinoma by Regulating miR-1297/ROCK1 Pathway |
title_sort |
circular rna hsa_circ_101141 as a competing endogenous rna facilitates tumorigenesis of hepatocellular carcinoma by regulating mir-1297/rock1 pathway |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
1555-3892 |
publishDate |
2020-08-01 |
description |
As a novel class of noncoding RNAs, circular RNAs (circRNAs) have been recently reported to be involved in cell development and function. However, the functional role of circRNAs in hepatocellular carcinoma (HCC) remains unclear. In the present study, we found that the expression of human circ_101141 was upregulated in HCC tissues and cells. In addition, downregulation of circ_101141 dramatically inhibited cell proliferation, migration, and invasion in HCC cells. In addition, by using the bioinformatics tools, the potential target of circ_101141 was predicted. Mechanistic investigations indicated that circ_101141 acted as a miR-1297 “sponge”; meanwhile, Rho-associated, coiled-coil-containing protein kinase 1 (ROCK1) was a direct target of miR-1297. Further experiments demonstrated that circ_101141 contributed to the progression of HCC by acting as competing endogenous RNA (ceRNA) of miR-1297 to regulate ROCK1 expression. Furthermore, knockdown of circ_101141 attenuated HCC tumorigenesis in vivo. Taken together, these findings indicated that circRNA circ_101141 acted as a ceRNA to facilitate tumorigenesis of HCC by regulating miR-1297/ROCK1 pathway. |
url |
https://doi.org/10.1177/0963689720948016 |
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