The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in Mitochondria
In land plants, the pentatricopeptide repeat (PPR) proteins form a large family involved in post-transcriptional processing of RNA in mitochondria and chloroplasts, which is critical for plant development and evolutionary adaption. Although studies showed a number of PPR proteins generally influence...
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doaj-ae424076b4444c5f9c46c7ab7a53e3f02020-11-25T03:03:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-06-011110.3389/fpls.2020.00749532785The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in MitochondriaQiannan Zhang0Yanghong Xu1Jishuai Huang2Kai Zhang3Kai Zhang4Haijun Xiao5Xiaojian Qin6Linlin Zhu7Yingguo Zhu8Jun Hu9State Key Laboratory of Hybrid Rice, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Sciences, Wuhan University, Wuhan, ChinaState Key Laboratory of Hybrid Rice, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Sciences, Wuhan University, Wuhan, ChinaState Key Laboratory of Hybrid Rice, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Sciences, Wuhan University, Wuhan, ChinaDepartment of Surgery, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, United StatesDepartment of Surgical Research, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, United StatesCollege of Life Sciences, Sichuan University, Chengdu, ChinaChongqing Key Laboratory of Molecular Biology of Plants Environmental Adaptations, College of Life Sciences, Chongqing Normal University, Chongqing, ChinaNo.9 Middle School of Zhengzhou, Zhengzhou, ChinaState Key Laboratory of Hybrid Rice, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Sciences, Wuhan University, Wuhan, ChinaState Key Laboratory of Hybrid Rice, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Sciences, Wuhan University, Wuhan, ChinaIn land plants, the pentatricopeptide repeat (PPR) proteins form a large family involved in post-transcriptional processing of RNA in mitochondria and chloroplasts, which is critical for plant development and evolutionary adaption. Although studies showed a number of PPR proteins generally influence the editing of organellar genes, few of them were characterized in detail in rice. Here, we report a PLS-E subclass PPR protein in rice, PPR756, loss of function of which led to the abolishment of RNA editing events among three mitochondrial genes including atp6, ccmC, and nad7. Their defective C-to-U transformation then resulted in improper amino acid retention which could cause abortive pollen development. Furthermore, PPR756 could bind to the three target genes directly and interact with three OsMORFs (multiple organellar RNA editing factors): OsMORF1, OsMORF8-1, and OsMORF8-2. The knock-out plants of PPR756 exhibited retarded growth and greener leaves during the early vegetative stages, along with sterile pollen and lower seed setting at the reproductive stage. These results established a role for PPR756 in rice development, participating in RNA editing of three various transcripts and cooperating with OsMORFs via an editosome manner in rice.https://www.frontiersin.org/article/10.3389/fpls.2020.00749/fullpentatricopeptide repeat (PPR) proteinsRNA editingmitochondriachloroplastssterilityOsMORFs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiannan Zhang Yanghong Xu Jishuai Huang Kai Zhang Kai Zhang Haijun Xiao Xiaojian Qin Linlin Zhu Yingguo Zhu Jun Hu |
spellingShingle |
Qiannan Zhang Yanghong Xu Jishuai Huang Kai Zhang Kai Zhang Haijun Xiao Xiaojian Qin Linlin Zhu Yingguo Zhu Jun Hu The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in Mitochondria Frontiers in Plant Science pentatricopeptide repeat (PPR) proteins RNA editing mitochondria chloroplasts sterility OsMORFs |
author_facet |
Qiannan Zhang Yanghong Xu Jishuai Huang Kai Zhang Kai Zhang Haijun Xiao Xiaojian Qin Linlin Zhu Yingguo Zhu Jun Hu |
author_sort |
Qiannan Zhang |
title |
The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in Mitochondria |
title_short |
The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in Mitochondria |
title_full |
The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in Mitochondria |
title_fullStr |
The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in Mitochondria |
title_full_unstemmed |
The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in Mitochondria |
title_sort |
rice pentatricopeptide repeat protein ppr756 is involved in pollen development by affecting multiple rna editing in mitochondria |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-06-01 |
description |
In land plants, the pentatricopeptide repeat (PPR) proteins form a large family involved in post-transcriptional processing of RNA in mitochondria and chloroplasts, which is critical for plant development and evolutionary adaption. Although studies showed a number of PPR proteins generally influence the editing of organellar genes, few of them were characterized in detail in rice. Here, we report a PLS-E subclass PPR protein in rice, PPR756, loss of function of which led to the abolishment of RNA editing events among three mitochondrial genes including atp6, ccmC, and nad7. Their defective C-to-U transformation then resulted in improper amino acid retention which could cause abortive pollen development. Furthermore, PPR756 could bind to the three target genes directly and interact with three OsMORFs (multiple organellar RNA editing factors): OsMORF1, OsMORF8-1, and OsMORF8-2. The knock-out plants of PPR756 exhibited retarded growth and greener leaves during the early vegetative stages, along with sterile pollen and lower seed setting at the reproductive stage. These results established a role for PPR756 in rice development, participating in RNA editing of three various transcripts and cooperating with OsMORFs via an editosome manner in rice. |
topic |
pentatricopeptide repeat (PPR) proteins RNA editing mitochondria chloroplasts sterility OsMORFs |
url |
https://www.frontiersin.org/article/10.3389/fpls.2020.00749/full |
work_keys_str_mv |
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