A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices
This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabricati...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-09-01
|
Series: | Micromachines |
Subjects: | |
Online Access: | https://www.mdpi.com/2072-666X/10/9/624 |
id |
doaj-ae42e55afd7142cdae713a0d56f835bb |
---|---|
record_format |
Article |
spelling |
doaj-ae42e55afd7142cdae713a0d56f835bb2020-11-25T02:08:00ZengMDPI AGMicromachines2072-666X2019-09-0110962410.3390/mi10090624mi10090624A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care DevicesTrieu Nguyen0Aaydha Chidambara Vinayaka1Dang Duong Bang2Anders Wolff3Department of Biotechnology and Biomedicine, Technical University of Denmark, DK 2800 Kgs. Lyngby, DenmarkLaboratory of Applied Micro and Nanotechnology (LAMINATE), Division of Microbiology and Production, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK 2800 Lyngby, DenmarkLaboratory of Applied Micro and Nanotechnology (LAMINATE), Division of Microbiology and Production, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK 2800 Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, DK 2800 Kgs. Lyngby, DenmarkThis protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of <i>Salmonella</i> (<i>Salmonella</i> Typhimurium and <i>Salmonella</i> Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR).https://www.mdpi.com/2072-666X/10/9/624polymermicrofluidic chipspoint of carethree-dimensional microstructuresprotocol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Trieu Nguyen Aaydha Chidambara Vinayaka Dang Duong Bang Anders Wolff |
spellingShingle |
Trieu Nguyen Aaydha Chidambara Vinayaka Dang Duong Bang Anders Wolff A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices Micromachines polymer microfluidic chips point of care three-dimensional microstructures protocol |
author_facet |
Trieu Nguyen Aaydha Chidambara Vinayaka Dang Duong Bang Anders Wolff |
author_sort |
Trieu Nguyen |
title |
A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices |
title_short |
A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices |
title_full |
A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices |
title_fullStr |
A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices |
title_full_unstemmed |
A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices |
title_sort |
complete protocol for rapid and low-cost fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2019-09-01 |
description |
This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of <i>Salmonella</i> (<i>Salmonella</i> Typhimurium and <i>Salmonella</i> Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR). |
topic |
polymer microfluidic chips point of care three-dimensional microstructures protocol |
url |
https://www.mdpi.com/2072-666X/10/9/624 |
work_keys_str_mv |
AT trieunguyen acompleteprotocolforrapidandlowcostfabricationofpolymermicrofluidicchipscontainingthreedimensionalmicrostructuresusedinpointofcaredevices AT aaydhachidambaravinayaka acompleteprotocolforrapidandlowcostfabricationofpolymermicrofluidicchipscontainingthreedimensionalmicrostructuresusedinpointofcaredevices AT dangduongbang acompleteprotocolforrapidandlowcostfabricationofpolymermicrofluidicchipscontainingthreedimensionalmicrostructuresusedinpointofcaredevices AT anderswolff acompleteprotocolforrapidandlowcostfabricationofpolymermicrofluidicchipscontainingthreedimensionalmicrostructuresusedinpointofcaredevices AT trieunguyen completeprotocolforrapidandlowcostfabricationofpolymermicrofluidicchipscontainingthreedimensionalmicrostructuresusedinpointofcaredevices AT aaydhachidambaravinayaka completeprotocolforrapidandlowcostfabricationofpolymermicrofluidicchipscontainingthreedimensionalmicrostructuresusedinpointofcaredevices AT dangduongbang completeprotocolforrapidandlowcostfabricationofpolymermicrofluidicchipscontainingthreedimensionalmicrostructuresusedinpointofcaredevices AT anderswolff completeprotocolforrapidandlowcostfabricationofpolymermicrofluidicchipscontainingthreedimensionalmicrostructuresusedinpointofcaredevices |
_version_ |
1724928166048301056 |