Engineering of family-5 glycoside hydrolase (Cel5A) from an uncultured bacterium for efficient hydrolysis of cellulosic substrates.
Cel5A, an endoglucanase, was derived from the metagenomic library of vermicompost. The deduced amino acid sequence of Cel5A shows high sequence homology with family-5 glycoside hydrolases, which contain a single catalytic domain but no distinct cellulose-binding domain. Random mutagenesis and cellul...
Main Authors: | Amar A Telke, Ningning Zhuang, Sunil S Ghatge, Sook-Hee Lee, Asad Ali Shah, Haji Khan, Youngsoon Um, Hyun-Dong Shin, Young Ryun Chung, Kon Ho Lee, Seon-Won Kim |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
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Series: | PLoS ONE |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23785445/?tool=EBI |
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