A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (<i>Lupinus angustifolius</i> L.)
Narrow-leafed lupin (<i>Lupinus angustifolius</i> L.) has recently been supplied with advanced genomic resources and, as such, has become a well-known model for molecular evolutionary studies within the legume family—a group of plants able to fix nitrogen from the atmosphere. The phyloge...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-04-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/7/2580 |
id |
doaj-ae5ac4766e4744e6b464de6f3fcac67b |
---|---|
record_format |
Article |
spelling |
doaj-ae5ac4766e4744e6b464de6f3fcac67b2020-11-25T02:21:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01212580258010.3390/ijms21072580A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (<i>Lupinus angustifolius</i> L.)Katarzyna B. Czyż0Michał Książkiewicz1Grzegorz Koczyk2Anna Szczepaniak3Jan Podkowiński4Barbara Naganowska5Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandDepartment of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandDepartment of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandDepartment of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandDepartment of Genomics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznan, PolandDepartment of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandNarrow-leafed lupin (<i>Lupinus angustifolius</i> L.) has recently been supplied with advanced genomic resources and, as such, has become a well-known model for molecular evolutionary studies within the legume family—a group of plants able to fix nitrogen from the atmosphere. The phylogenetic position of lupins in Papilionoideae and their evolutionary distance to other higher plants facilitates the use of this model species to improve our knowledge on genes involved in nitrogen assimilation and primary metabolism, providing novel contributions to our understanding of the evolutionary history of legumes. In this study, we present a complex characterization of two narrow-leafed lupin gene families—glutamine synthetase (<i>GS</i>) and phosphoenolpyruvate carboxylase (<i>PEPC</i>). We combine a comparative analysis of gene structures and a synteny-based approach with phylogenetic reconstruction and reconciliation of the gene family and species history in order to examine events underlying the extant diversity of both families. Employing the available evidence, we show the impact of duplications on the initial complement of the analyzed gene families within the genistoid clade and posit that the function of duplicates has been largely retained. In terms of a broader perspective, our results concerning <i>GS</i> and <i>PEPC</i> gene families corroborate earlier findings pointing to key whole genome duplication/triplication event(s) affecting the genistoid lineage.https://www.mdpi.com/1422-0067/21/7/2580Fabaceae<i>Lupinus</i>glutamine synthetase (<i>GS</i>)phosphoenolpyruvate carboxylase (<i>PEPC</i>)phylogenyevolution |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katarzyna B. Czyż Michał Książkiewicz Grzegorz Koczyk Anna Szczepaniak Jan Podkowiński Barbara Naganowska |
spellingShingle |
Katarzyna B. Czyż Michał Książkiewicz Grzegorz Koczyk Anna Szczepaniak Jan Podkowiński Barbara Naganowska A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (<i>Lupinus angustifolius</i> L.) International Journal of Molecular Sciences Fabaceae <i>Lupinus</i> glutamine synthetase (<i>GS</i>) phosphoenolpyruvate carboxylase (<i>PEPC</i>) phylogeny evolution |
author_facet |
Katarzyna B. Czyż Michał Książkiewicz Grzegorz Koczyk Anna Szczepaniak Jan Podkowiński Barbara Naganowska |
author_sort |
Katarzyna B. Czyż |
title |
A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (<i>Lupinus angustifolius</i> L.) |
title_short |
A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (<i>Lupinus angustifolius</i> L.) |
title_full |
A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (<i>Lupinus angustifolius</i> L.) |
title_fullStr |
A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (<i>Lupinus angustifolius</i> L.) |
title_full_unstemmed |
A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (<i>Lupinus angustifolius</i> L.) |
title_sort |
tale of two families: whole genome and segmental duplications underlie glutamine synthetase and phosphoenolpyruvate carboxylase diversity in narrow-leafed lupin (<i>lupinus angustifolius</i> l.) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-04-01 |
description |
Narrow-leafed lupin (<i>Lupinus angustifolius</i> L.) has recently been supplied with advanced genomic resources and, as such, has become a well-known model for molecular evolutionary studies within the legume family—a group of plants able to fix nitrogen from the atmosphere. The phylogenetic position of lupins in Papilionoideae and their evolutionary distance to other higher plants facilitates the use of this model species to improve our knowledge on genes involved in nitrogen assimilation and primary metabolism, providing novel contributions to our understanding of the evolutionary history of legumes. In this study, we present a complex characterization of two narrow-leafed lupin gene families—glutamine synthetase (<i>GS</i>) and phosphoenolpyruvate carboxylase (<i>PEPC</i>). We combine a comparative analysis of gene structures and a synteny-based approach with phylogenetic reconstruction and reconciliation of the gene family and species history in order to examine events underlying the extant diversity of both families. Employing the available evidence, we show the impact of duplications on the initial complement of the analyzed gene families within the genistoid clade and posit that the function of duplicates has been largely retained. In terms of a broader perspective, our results concerning <i>GS</i> and <i>PEPC</i> gene families corroborate earlier findings pointing to key whole genome duplication/triplication event(s) affecting the genistoid lineage. |
topic |
Fabaceae <i>Lupinus</i> glutamine synthetase (<i>GS</i>) phosphoenolpyruvate carboxylase (<i>PEPC</i>) phylogeny evolution |
url |
https://www.mdpi.com/1422-0067/21/7/2580 |
work_keys_str_mv |
AT katarzynabczyz ataleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT michałksiazkiewicz ataleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT grzegorzkoczyk ataleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT annaszczepaniak ataleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT janpodkowinski ataleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT barbaranaganowska ataleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT katarzynabczyz taleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT michałksiazkiewicz taleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT grzegorzkoczyk taleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT annaszczepaniak taleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT janpodkowinski taleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil AT barbaranaganowska taleoftwofamilieswholegenomeandsegmentalduplicationsunderlieglutaminesynthetaseandphosphoenolpyruvatecarboxylasediversityinnarrowleafedlupinilupinusangustifoliusil |
_version_ |
1724864336560652288 |