The physiological characteristics of the basal microvilli microvessels in pancreatic cancers
Abstract Pancreatic cancer (PC) is a highly lethal tumor with controversial high glucose uptake and hypomicrovascularity, and the hypomicrovasculature, which is considered to have poor perfusion, blocks the delivery of drugs to tumors. The preferential existence of a novel endothelial projection wit...
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2020-08-01
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Series: | Cancer Medicine |
Online Access: | https://doi.org/10.1002/cam4.3177 |
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doaj-ae644067eba64cfdafa36abd3d26105d2020-11-25T03:18:42ZengWileyCancer Medicine2045-76342020-08-019155535554510.1002/cam4.3177The physiological characteristics of the basal microvilli microvessels in pancreatic cancersLixiang Ma0Xu Han1Jichun Gu2Ji Li3Wenhui Lou4Chen Jin5Hexige Saiyin6Department of Anatomy, Histology & Embryology School of Medical Sciences Fudan University Shanghai ChinaGeneral Surgery Department Zhongshan Hospital Fudan University Shanghai ChinaDepartment of Pancreatic Surgery Huashan Hospital Fudan University Shanghai ChinaDepartment of Pancreatic Surgery Huashan Hospital Fudan University Shanghai ChinaGeneral Surgery Department Zhongshan Hospital Fudan University Shanghai ChinaDepartment of Pancreatic Surgery Huashan Hospital Fudan University Shanghai ChinaThe State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai ChinaAbstract Pancreatic cancer (PC) is a highly lethal tumor with controversial high glucose uptake and hypomicrovascularity, and the hypomicrovasculature, which is considered to have poor perfusion, blocks the delivery of drugs to tumors. The preferential existence of a novel endothelial projection with trafficking vesicles in PCs, referring to basal microvilli, was described previously. However, the perfusion and nutrients delivering status of the basal microvilli microvessels are unknown. Here, we used the perfusion of fluorescently labeled CD31 antibody, lectin, and 2‐NBDG to autochthonous PC‐bearing mice, immunostaining, probe‐based confocal laser endoscopy and three‐dimensional (3D) reconstruction to study the nutrient trafficking, and perfusion status of the basal microvilli microvasculature in PC. Our data showed that the coperfusion of lectin and CD31 is an efficient way to show the microcirculation in most healthy organs. However, coperfusion with lectin and CD31 is inefficient for showing the microcirculation in PCs compared with that in healthy organs and immunostaining. This method does not reflect the nutrient trafficking status in the microvessels, especially in basal microvilli microvessels of PCs. In basal microvilli microvessels that were poorly labeled by lectin, we observed large vesicle‐like structures with 2‐NBDG preferentially located at the base of the basal microvilli or in basal microvilli, and there were long filopodia on the luminal surface of the human PC microvasculature. Our observations suggest that the PC microvasculature, especially basal microvilli microvessels, is well perfused and might be highly efficient in the trafficking of glucose or other nutrients, indicating that macropinocytosis might participate in the nutrient trafficking.https://doi.org/10.1002/cam4.3177 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lixiang Ma Xu Han Jichun Gu Ji Li Wenhui Lou Chen Jin Hexige Saiyin |
spellingShingle |
Lixiang Ma Xu Han Jichun Gu Ji Li Wenhui Lou Chen Jin Hexige Saiyin The physiological characteristics of the basal microvilli microvessels in pancreatic cancers Cancer Medicine |
author_facet |
Lixiang Ma Xu Han Jichun Gu Ji Li Wenhui Lou Chen Jin Hexige Saiyin |
author_sort |
Lixiang Ma |
title |
The physiological characteristics of the basal microvilli microvessels in pancreatic cancers |
title_short |
The physiological characteristics of the basal microvilli microvessels in pancreatic cancers |
title_full |
The physiological characteristics of the basal microvilli microvessels in pancreatic cancers |
title_fullStr |
The physiological characteristics of the basal microvilli microvessels in pancreatic cancers |
title_full_unstemmed |
The physiological characteristics of the basal microvilli microvessels in pancreatic cancers |
title_sort |
physiological characteristics of the basal microvilli microvessels in pancreatic cancers |
publisher |
Wiley |
series |
Cancer Medicine |
issn |
2045-7634 |
publishDate |
2020-08-01 |
description |
Abstract Pancreatic cancer (PC) is a highly lethal tumor with controversial high glucose uptake and hypomicrovascularity, and the hypomicrovasculature, which is considered to have poor perfusion, blocks the delivery of drugs to tumors. The preferential existence of a novel endothelial projection with trafficking vesicles in PCs, referring to basal microvilli, was described previously. However, the perfusion and nutrients delivering status of the basal microvilli microvessels are unknown. Here, we used the perfusion of fluorescently labeled CD31 antibody, lectin, and 2‐NBDG to autochthonous PC‐bearing mice, immunostaining, probe‐based confocal laser endoscopy and three‐dimensional (3D) reconstruction to study the nutrient trafficking, and perfusion status of the basal microvilli microvasculature in PC. Our data showed that the coperfusion of lectin and CD31 is an efficient way to show the microcirculation in most healthy organs. However, coperfusion with lectin and CD31 is inefficient for showing the microcirculation in PCs compared with that in healthy organs and immunostaining. This method does not reflect the nutrient trafficking status in the microvessels, especially in basal microvilli microvessels of PCs. In basal microvilli microvessels that were poorly labeled by lectin, we observed large vesicle‐like structures with 2‐NBDG preferentially located at the base of the basal microvilli or in basal microvilli, and there were long filopodia on the luminal surface of the human PC microvasculature. Our observations suggest that the PC microvasculature, especially basal microvilli microvessels, is well perfused and might be highly efficient in the trafficking of glucose or other nutrients, indicating that macropinocytosis might participate in the nutrient trafficking. |
url |
https://doi.org/10.1002/cam4.3177 |
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