Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China
ABSTRACT: Avian infectious bronchitis (IB), caused by avian infectious bronchitis virus (IBV), is an acute and highly contagious disease that is extremely harmful to the poultry industry throughout the world. The cross-using of different attenuated live vaccine strains has led to the occurrence of d...
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Elsevier
2021-07-01
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Series: | Poultry Science |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121002443 |
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doaj-ae96764e89414236bb839d3f4c5e1116 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenjun Yan Rongbin Qiu Fuyan Wang Xue Fu Hao Li Pengfei Cui Yaru Zhai Chun Li Lan Zhang Kui Gu Lei Zuo Changwei Lei Hongning Wang Xin Yang |
spellingShingle |
Wenjun Yan Rongbin Qiu Fuyan Wang Xue Fu Hao Li Pengfei Cui Yaru Zhai Chun Li Lan Zhang Kui Gu Lei Zuo Changwei Lei Hongning Wang Xin Yang Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China Poultry Science infectious bronchitis virus recombination pathogenicity |
author_facet |
Wenjun Yan Rongbin Qiu Fuyan Wang Xue Fu Hao Li Pengfei Cui Yaru Zhai Chun Li Lan Zhang Kui Gu Lei Zuo Changwei Lei Hongning Wang Xin Yang |
author_sort |
Wenjun Yan |
title |
Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China |
title_short |
Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China |
title_full |
Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China |
title_fullStr |
Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China |
title_full_unstemmed |
Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China |
title_sort |
genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from gi-1, gi-13, gi-28, and gi-19 strains in southwestern china |
publisher |
Elsevier |
series |
Poultry Science |
issn |
0032-5791 |
publishDate |
2021-07-01 |
description |
ABSTRACT: Avian infectious bronchitis (IB), caused by avian infectious bronchitis virus (IBV), is an acute and highly contagious disease that is extremely harmful to the poultry industry throughout the world. The cross-using of different attenuated live vaccine strains has led to the occurrence of diverse IBV serotypes. In this study, we isolated an IBV strain from a chicken farm in southwest China and designated it CK/CH/SCMY/160315. Construction of a phylogenetic tree based on full S1 gene sequence analysis suggested that CK/CH/SCMY/160315 bears similarity to GI-28, and further comparison of S1 amino acid residues revealed that CK/CH/SCMY/160315 showed mutations and deletions in many key positions between LDT3-A and other GI-28 reference strains. Importantly, CK/CH/SCMY/160315 was identified as a novel recombinant virus derived from live attenuated vaccine strains H120 (GI-1), 4/91 (GI-13), LDT3-A (GI-28), and the field strain LJL/08-1 (GI-19), identifying at least 5 recombination sites in both structural and accessory genes. Pathogenicity analysis indicated that CK/CH/SCMY/160315 caused listlessness, sneezing, huddling, head shaking, and increased antibody levels in the inoculated chickens. To further describe pathogenicity of this novel strain, we assessed viral load in different tissues and conducted hematoxylin and eosin (HE) staining of the trachea, lungs and kidneys. Our results provide evidence for the continuing evolution of IBV field strains via genetic recombination and mutation, leading to outbreaks in the vaccinated chicken populations in China. |
topic |
infectious bronchitis virus recombination pathogenicity |
url |
http://www.sciencedirect.com/science/article/pii/S0032579121002443 |
work_keys_str_mv |
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doaj-ae96764e89414236bb839d3f4c5e11162021-07-01T04:31:15ZengElsevierPoultry Science0032-57912021-07-011007101210Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern ChinaWenjun Yan0Rongbin Qiu1Fuyan Wang2Xue Fu3Hao Li4Pengfei Cui5Yaru Zhai6Chun Li7Lan Zhang8Kui Gu9Lei Zuo10Changwei Lei11Hongning Wang12Xin Yang13Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSichuan Sundaily Farm Ecological Food Co., Ltd., Mianyang 621010, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaSichuan Animal Disease Control Center, Chengdu, 610041, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, China; Corresponding author:ABSTRACT: Avian infectious bronchitis (IB), caused by avian infectious bronchitis virus (IBV), is an acute and highly contagious disease that is extremely harmful to the poultry industry throughout the world. The cross-using of different attenuated live vaccine strains has led to the occurrence of diverse IBV serotypes. In this study, we isolated an IBV strain from a chicken farm in southwest China and designated it CK/CH/SCMY/160315. Construction of a phylogenetic tree based on full S1 gene sequence analysis suggested that CK/CH/SCMY/160315 bears similarity to GI-28, and further comparison of S1 amino acid residues revealed that CK/CH/SCMY/160315 showed mutations and deletions in many key positions between LDT3-A and other GI-28 reference strains. Importantly, CK/CH/SCMY/160315 was identified as a novel recombinant virus derived from live attenuated vaccine strains H120 (GI-1), 4/91 (GI-13), LDT3-A (GI-28), and the field strain LJL/08-1 (GI-19), identifying at least 5 recombination sites in both structural and accessory genes. Pathogenicity analysis indicated that CK/CH/SCMY/160315 caused listlessness, sneezing, huddling, head shaking, and increased antibody levels in the inoculated chickens. To further describe pathogenicity of this novel strain, we assessed viral load in different tissues and conducted hematoxylin and eosin (HE) staining of the trachea, lungs and kidneys. Our results provide evidence for the continuing evolution of IBV field strains via genetic recombination and mutation, leading to outbreaks in the vaccinated chicken populations in China.http://www.sciencedirect.com/science/article/pii/S0032579121002443infectious bronchitis virusrecombinationpathogenicity |