Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression
Abstract Integrin β1 is known to be involved in differentiation, migration, proliferation, wound repair, tissue development, and organogenesis. In order to analyze the binding probability between integrin β1 ligand and cluster of differentiation 29 (CD29) receptors, atomic force microscopy (AFM) was...
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doaj-ae99e32678654dac92f76395257f6f532020-11-25T02:45:14ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2018-10-0113111110.1186/s11671-018-2722-zAtomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 ExpressionJie Yang0Ming-Tang He1Xun Huang2Qiu-Shi Wang3Jiang Pi4Hua-Jun Wang5Ali Hasan Rahhal6Si-Min Luo7Zhen-Gang Zha8Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversityLonggang Orthopedics Hospital of ShenzhenInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversityInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversityDepartment of Microbiology and Immunology, University of Illinois at ChicagoInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversityInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversityInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversityInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversityAbstract Integrin β1 is known to be involved in differentiation, migration, proliferation, wound repair, tissue development, and organogenesis. In order to analyze the binding probability between integrin β1 ligand and cluster of differentiation 29 (CD29) receptors, atomic force microscopy (AFM) was used to detect native integrin β1-coupled receptors on the surface of human adipose-derived stem cells (hADSc). The binding probability of integrin β1 ligand–receptor interaction was probed by integrin β1-functionalized tips on hADSc during early chondrogenic differentiation at the two-dimensional cell culture level. Cell morphology and ultrastructure of hADSc were measured by AFM, which demonstrated that long spindled cells became polygonal cells with decreased length/width ratios and increased roughness during chondrogenic induction. The binding of integrin β1 ligand and CD29 receptors was detected by β1-functionalized tips for living hADSc. A total of 1200 curves were recorded at 0, 6, and 12 days of chondrogenic induction. Average rupture forces were, respectively, 61.8 ± 22.2 pN, 60 ± 20.2 pN, and 67.2 ± 22.0 pN. Rupture events were 19.58 ± 1.74%, 28.03 ± 2.05%, and 33.4 ± 1.89%, respectively, which demonstrated that binding probability was increased between integrin β1 ligand and receptors on the surface of hADSc during chondrogenic induction. Integrin β1 and the β-catenin/SOX signaling pathway were correlated during chondrogenic differentiation. The results of this investigation imply that AFM offers kinetic and visual insight into the changes in integrin β1 ligand–CD29 receptor binding on hADSc during chondrogenesis. Changes in cellular morphology, membrane ultrastructure, and the probability of ligand–transmembrane receptor binding were demonstrated to be useful markers for evaluation of the chondrogenic differentiation process.http://link.springer.com/article/10.1186/s11671-018-2722-zAtomic force microscopyChondrogenic differentiationIntegrin β1Human adipose-derived stem cellsβ-catenin/SOX signaling pathway |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jie Yang Ming-Tang He Xun Huang Qiu-Shi Wang Jiang Pi Hua-Jun Wang Ali Hasan Rahhal Si-Min Luo Zhen-Gang Zha |
spellingShingle |
Jie Yang Ming-Tang He Xun Huang Qiu-Shi Wang Jiang Pi Hua-Jun Wang Ali Hasan Rahhal Si-Min Luo Zhen-Gang Zha Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression Nanoscale Research Letters Atomic force microscopy Chondrogenic differentiation Integrin β1 Human adipose-derived stem cells β-catenin/SOX signaling pathway |
author_facet |
Jie Yang Ming-Tang He Xun Huang Qiu-Shi Wang Jiang Pi Hua-Jun Wang Ali Hasan Rahhal Si-Min Luo Zhen-Gang Zha |
author_sort |
Jie Yang |
title |
Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression |
title_short |
Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression |
title_full |
Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression |
title_fullStr |
Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression |
title_full_unstemmed |
Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression |
title_sort |
atomic force microscopy-based nanoscopy of chondrogenically differentiating human adipose-derived stem cells: nanostructure and integrin β1 expression |
publisher |
SpringerOpen |
series |
Nanoscale Research Letters |
issn |
1931-7573 1556-276X |
publishDate |
2018-10-01 |
description |
Abstract Integrin β1 is known to be involved in differentiation, migration, proliferation, wound repair, tissue development, and organogenesis. In order to analyze the binding probability between integrin β1 ligand and cluster of differentiation 29 (CD29) receptors, atomic force microscopy (AFM) was used to detect native integrin β1-coupled receptors on the surface of human adipose-derived stem cells (hADSc). The binding probability of integrin β1 ligand–receptor interaction was probed by integrin β1-functionalized tips on hADSc during early chondrogenic differentiation at the two-dimensional cell culture level. Cell morphology and ultrastructure of hADSc were measured by AFM, which demonstrated that long spindled cells became polygonal cells with decreased length/width ratios and increased roughness during chondrogenic induction. The binding of integrin β1 ligand and CD29 receptors was detected by β1-functionalized tips for living hADSc. A total of 1200 curves were recorded at 0, 6, and 12 days of chondrogenic induction. Average rupture forces were, respectively, 61.8 ± 22.2 pN, 60 ± 20.2 pN, and 67.2 ± 22.0 pN. Rupture events were 19.58 ± 1.74%, 28.03 ± 2.05%, and 33.4 ± 1.89%, respectively, which demonstrated that binding probability was increased between integrin β1 ligand and receptors on the surface of hADSc during chondrogenic induction. Integrin β1 and the β-catenin/SOX signaling pathway were correlated during chondrogenic differentiation. The results of this investigation imply that AFM offers kinetic and visual insight into the changes in integrin β1 ligand–CD29 receptor binding on hADSc during chondrogenesis. Changes in cellular morphology, membrane ultrastructure, and the probability of ligand–transmembrane receptor binding were demonstrated to be useful markers for evaluation of the chondrogenic differentiation process. |
topic |
Atomic force microscopy Chondrogenic differentiation Integrin β1 Human adipose-derived stem cells β-catenin/SOX signaling pathway |
url |
http://link.springer.com/article/10.1186/s11671-018-2722-z |
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