Direct shoot regeneration of three Petunia cultivars
A tissue culture system for acquiring high efficiency regeneration of Petunia was optimized. Leaf explants of Alvan, Large Flower Alvan (LF Alvan) and Mahalat cultivars of Petunia hybrida were cultured separately on MS medium including various concentrations of TDZ and BA without auxin in order to...
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doaj-ae9bdcc78c3443fc987291799c9ca22e2020-11-25T01:30:40ZengFirenze University PressAdvances in Horticultural Science0394-61691592-15732019-10-0133310.13128/ahs-24026Direct shoot regeneration of three Petunia cultivarsA.N. Vakili0Hedayat Bagheri1P. Azadi2Department of Biotechnology, Bu Ali-Sina University, 6517838695, HamedamDepartment of Biotechnology, Bu Ali-Sina University, 6517838695, HamedamDepartment of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj A tissue culture system for acquiring high efficiency regeneration of Petunia was optimized. Leaf explants of Alvan, Large Flower Alvan (LF Alvan) and Mahalat cultivars of Petunia hybrida were cultured separately on MS medium including various concentrations of TDZ and BA without auxin in order to assess direct shoot regeneration. Alvan showed the highest frequency of shoot regeneration (100%) and the highest mean number of shoots per explant (25.33) on MS containing 2 mg/l TDZ. For LF Alvan cultivar the highest percentage of shoot organogenesis (100%) and the highest mean number of shoots per explant (18.20) were observed when MS medium containing 1 mg/l BA was used. With the Mahalat cultivar the maximum rate of direct regeneration was obtained on MS supplemented with 0.5 and 1 mg/l BA (80%). The mean number of shoots per explant (9.63) was obtained when 2 mg/l TDZ was used. Regenerated shoots were successfully elongated (2 to 3 cm in length) and transferred into half-strength MS as the rooting medium supplemented with 0.1 mg/l NAA. The shoots were successfully rooted, acclimatized and transferred to the greenhouse. https://oaj.fupress.net/index.php/ahs/article/view/7371BAPleaf diskorganogenesisPetunia hybridaTDZ |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
A.N. Vakili Hedayat Bagheri P. Azadi |
spellingShingle |
A.N. Vakili Hedayat Bagheri P. Azadi Direct shoot regeneration of three Petunia cultivars Advances in Horticultural Science BAP leaf disk organogenesis Petunia hybrida TDZ |
author_facet |
A.N. Vakili Hedayat Bagheri P. Azadi |
author_sort |
A.N. Vakili |
title |
Direct shoot regeneration of three Petunia cultivars |
title_short |
Direct shoot regeneration of three Petunia cultivars |
title_full |
Direct shoot regeneration of three Petunia cultivars |
title_fullStr |
Direct shoot regeneration of three Petunia cultivars |
title_full_unstemmed |
Direct shoot regeneration of three Petunia cultivars |
title_sort |
direct shoot regeneration of three petunia cultivars |
publisher |
Firenze University Press |
series |
Advances in Horticultural Science |
issn |
0394-6169 1592-1573 |
publishDate |
2019-10-01 |
description |
A tissue culture system for acquiring high efficiency regeneration of Petunia was optimized. Leaf explants of Alvan, Large Flower Alvan (LF Alvan) and Mahalat cultivars of Petunia hybrida were cultured separately on MS medium including various concentrations of TDZ and BA without auxin in order to assess direct shoot regeneration. Alvan showed the highest frequency of shoot regeneration (100%) and the highest mean number of shoots per explant (25.33) on MS containing 2 mg/l TDZ. For LF Alvan cultivar the highest percentage of shoot organogenesis (100%) and the highest mean number of shoots per explant (18.20) were observed when MS medium containing 1 mg/l BA was used. With the Mahalat cultivar the maximum rate of direct regeneration was obtained on MS supplemented with 0.5 and 1 mg/l BA (80%). The mean number of shoots per explant (9.63) was obtained when 2 mg/l TDZ was used. Regenerated shoots were successfully elongated (2 to 3 cm in length) and transferred into half-strength MS as the rooting medium supplemented with 0.1 mg/l NAA. The shoots were successfully rooted, acclimatized and transferred to the greenhouse.
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topic |
BAP leaf disk organogenesis Petunia hybrida TDZ |
url |
https://oaj.fupress.net/index.php/ahs/article/view/7371 |
work_keys_str_mv |
AT anvakili directshootregenerationofthreepetuniacultivars AT hedayatbagheri directshootregenerationofthreepetuniacultivars AT pazadi directshootregenerationofthreepetuniacultivars |
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1725090655652282368 |