Efficiency of biparental crossing in sugarcane analyzed by SSR markers
Sugarcane has hermaphrodite flowers, however, selfing and cross pollination may occur, resulting in selfed or hybrid progeny. The aim of this study was to analyze the paternity of progenies from biparental crosses, in order to identify true hybrids or progenies originating from pollen of unknown...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Brazilian Society of Plant Breeding
2014-07-01
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Series: | Crop Breeding and Applied Biotechnology |
Subjects: | |
Online Access: | http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-e387-02e1.pdf |
Summary: | Sugarcane has hermaphrodite flowers, however, selfing and cross pollination may occur, resulting in selfed or hybrid progeny.
The aim of this study was to analyze the paternity of progenies from biparental crosses, in order to identify true hybrids or progenies
originating from pollen of unknown origin. Seventy-six progenies from four crosses were analyzed using three highly polymorphic
microsatellite markers (SSR). Progenies showed moderate genetic similarity and were grouped into four distinct groups, according
to the crosses. Transmission of alleles from parents to offspring was clearly observed, in which selfed individuals were not observed,
and only true hybrids or progeny resulting from fertilization with pollen uncommon to both parents were. Results showed that there
was contamination with pollen from unknown parents in sugarcane crosses, suggesting that errors in the pedigree may occur, and
adjustment in the crossing procedure would decrease progenies from pollen of unknown origin. |
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ISSN: | 1518-7853 1518-7853 |