Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging
3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D im...
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doaj-aea93464a7464b7cb3c4931cd9e4be902020-11-25T03:20:38ZengMDPI AGMicromachines2072-666X2020-06-011158258210.3390/mi11060582Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic ImagingDongwoo Lee0Jihye Kim1Eunjoo Song2Ji-Young Jeong3Eun-chae Jeon4Pilhan Kim5Wonhee Lee6Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, KoreaGraduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, KoreaGraduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, KoreaDepartment of Nano Manufacturing Technology, Korea Institute of Machinery & Materials (KIMM),Daejeon 34103, KoreaSchool of Materials Science and Engineering, University of Ulsan, Ulsan 44776, KoreaGraduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, KoreaGraduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D imaging tool that is compatible with most conventional microscopes by integrating micromirrors and microchannel structures on coverslips to provide bidirectional imaging. Microfabricated micromirrors had a precisely 45° reflection angle and optically clean reflective surfaces with high reflectance over 95%. The micromirrors were embedded on coverslips that could be assembled as a microchannel structure. We demonstrated that this simple disposable device allows a conventional microscope to perform bidirectional imaging with simple control of a focal plane. Images of microbeads and cells under bright-field and fluorescent microscopy show that the device can provide a quick analysis of 3D information, such as 3D positions and subcellular structures.https://www.mdpi.com/2072-666X/11/6/582micromirrormicrofabrication3D imagingOptical MEMS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dongwoo Lee Jihye Kim Eunjoo Song Ji-Young Jeong Eun-chae Jeon Pilhan Kim Wonhee Lee |
spellingShingle |
Dongwoo Lee Jihye Kim Eunjoo Song Ji-Young Jeong Eun-chae Jeon Pilhan Kim Wonhee Lee Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging Micromachines micromirror microfabrication 3D imaging Optical MEMS |
author_facet |
Dongwoo Lee Jihye Kim Eunjoo Song Ji-Young Jeong Eun-chae Jeon Pilhan Kim Wonhee Lee |
author_sort |
Dongwoo Lee |
title |
Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging |
title_short |
Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging |
title_full |
Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging |
title_fullStr |
Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging |
title_full_unstemmed |
Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging |
title_sort |
micromirror-embedded coverslip assembly for bidirectional microscopic imaging |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2020-06-01 |
description |
3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D imaging tool that is compatible with most conventional microscopes by integrating micromirrors and microchannel structures on coverslips to provide bidirectional imaging. Microfabricated micromirrors had a precisely 45° reflection angle and optically clean reflective surfaces with high reflectance over 95%. The micromirrors were embedded on coverslips that could be assembled as a microchannel structure. We demonstrated that this simple disposable device allows a conventional microscope to perform bidirectional imaging with simple control of a focal plane. Images of microbeads and cells under bright-field and fluorescent microscopy show that the device can provide a quick analysis of 3D information, such as 3D positions and subcellular structures. |
topic |
micromirror microfabrication 3D imaging Optical MEMS |
url |
https://www.mdpi.com/2072-666X/11/6/582 |
work_keys_str_mv |
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