Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells
Staphylococcus epidermidis biofilm formation on indwelling medical devices is frequently associated with the development of chronic infections. Nevertheless, it has been suggested that cells released from these biofilms may induce severe acute infections with bacteraemia as one of its major associat...
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doaj-aecd2f73c95047c0ab1c6b522dcd82ab2020-11-24T23:58:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-09-01710.3389/fmicb.2016.01530209296Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cellsÂngela França0Ângela França1Begoña Pérez-Cabezas2Alexandra Correia3Gerald B. Pier4Nuno Cerca5Manuel Vilanova6Manuel Vilanova7Universidade do MinhoUniversidade do PortoUniversidade do PortoUniversidade do PortoBrigham and Women's Hospital/Harvard Medical SchoolUniversidade do MinhoUniversidade do PortoUniversidade do PortoStaphylococcus epidermidis biofilm formation on indwelling medical devices is frequently associated with the development of chronic infections. Nevertheless, it has been suggested that cells released from these biofilms may induce severe acute infections with bacteraemia as one of its major associated clinical manifestations. However, how biofilm-released cells interact with the host remains unclear. Here, using a murine model of hematogenously disseminated infection, we characterized the interaction of cells released from S. epidermidis biofilms with the immune system. Gene expression analysis of mouse splenocytes suggested that biofilm-released cells might be particularly effective at activating inflammatory and antigen presenting cells and inducing cellular apoptosis. Furthermore, biofilm-released cells induced a higher production of pro-inflammatory cytokines, in contrast to mice infected with planktonic cells, even though these had a similar bacterial load in livers and spleens. Overall, these results not only provide insights into the understanding of the role of biofilm-released cells in S. epidermidis biofilm-related infections and pathogenesis, but may also help explain the relapsing character of these infections.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01530/fullS. epidermidis biofilmsTissue colonizationBiofilm-released cellsmice spleen microarraySerum pro-inflammatory cytokines |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ângela França Ângela França Begoña Pérez-Cabezas Alexandra Correia Gerald B. Pier Nuno Cerca Manuel Vilanova Manuel Vilanova |
spellingShingle |
Ângela França Ângela França Begoña Pérez-Cabezas Alexandra Correia Gerald B. Pier Nuno Cerca Manuel Vilanova Manuel Vilanova Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells Frontiers in Microbiology S. epidermidis biofilms Tissue colonization Biofilm-released cells mice spleen microarray Serum pro-inflammatory cytokines |
author_facet |
Ângela França Ângela França Begoña Pérez-Cabezas Alexandra Correia Gerald B. Pier Nuno Cerca Manuel Vilanova Manuel Vilanova |
author_sort |
Ângela França |
title |
Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells |
title_short |
Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells |
title_full |
Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells |
title_fullStr |
Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells |
title_full_unstemmed |
Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells |
title_sort |
staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2016-09-01 |
description |
Staphylococcus epidermidis biofilm formation on indwelling medical devices is frequently associated with the development of chronic infections. Nevertheless, it has been suggested that cells released from these biofilms may induce severe acute infections with bacteraemia as one of its major associated clinical manifestations. However, how biofilm-released cells interact with the host remains unclear. Here, using a murine model of hematogenously disseminated infection, we characterized the interaction of cells released from S. epidermidis biofilms with the immune system. Gene expression analysis of mouse splenocytes suggested that biofilm-released cells might be particularly effective at activating inflammatory and antigen presenting cells and inducing cellular apoptosis. Furthermore, biofilm-released cells induced a higher production of pro-inflammatory cytokines, in contrast to mice infected with planktonic cells, even though these had a similar bacterial load in livers and spleens. Overall, these results not only provide insights into the understanding of the role of biofilm-released cells in S. epidermidis biofilm-related infections and pathogenesis, but may also help explain the relapsing character of these infections. |
topic |
S. epidermidis biofilms Tissue colonization Biofilm-released cells mice spleen microarray Serum pro-inflammatory cytokines |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01530/full |
work_keys_str_mv |
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