A subset of mouse colonic goblet cells expresses the bitter taste receptor Tas2r131.
The concept that gut nutrient sensing involves taste receptors has been fueled by recent reports associating the expression of taste receptors and taste-associated signaling molecules in the gut and in gut-derived cell lines with physiological responses induced by known taste stimuli. However, for b...
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doaj-aeed79a29adf46e6b094f67100d97ecf2020-11-25T00:47:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8282010.1371/journal.pone.0082820A subset of mouse colonic goblet cells expresses the bitter taste receptor Tas2r131.Simone PrandiMarta BromkeSandra HübnerAnja VoigtUlrich BoehmWolfgang MeyerhofMaik BehrensThe concept that gut nutrient sensing involves taste receptors has been fueled by recent reports associating the expression of taste receptors and taste-associated signaling molecules in the gut and in gut-derived cell lines with physiological responses induced by known taste stimuli. However, for bitter taste receptors (Tas2rs), direct evidence for their functional role in gut physiology is scarce and their cellular expression pattern remained unknown. We therefore investigated Tas2r expression in mice. RT-PCR experiments assessed the presence of mRNA for Tas2rs and taste signaling molecules in the gut. A gene-targeted mouse strain was established to visualize and identify cell types expressing the bitter receptor Tas2r131. Messenger RNA for various Tas2rs and taste signaling molecules were detected by RT-PCR in the gut. Using our knock-in mouse strain we demonstrate that a subset of colonic goblet cells express Tas2r131. Cells that express this receptor are absent in the upper gut and do not correspond to enteroendocrine and brush cells. Expression in colonic goblet cells is consistent with a role of Tas2rs in defense mechanisms against potentially harmful xenobiotics.http://europepmc.org/articles/PMC3867391?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simone Prandi Marta Bromke Sandra Hübner Anja Voigt Ulrich Boehm Wolfgang Meyerhof Maik Behrens |
spellingShingle |
Simone Prandi Marta Bromke Sandra Hübner Anja Voigt Ulrich Boehm Wolfgang Meyerhof Maik Behrens A subset of mouse colonic goblet cells expresses the bitter taste receptor Tas2r131. PLoS ONE |
author_facet |
Simone Prandi Marta Bromke Sandra Hübner Anja Voigt Ulrich Boehm Wolfgang Meyerhof Maik Behrens |
author_sort |
Simone Prandi |
title |
A subset of mouse colonic goblet cells expresses the bitter taste receptor Tas2r131. |
title_short |
A subset of mouse colonic goblet cells expresses the bitter taste receptor Tas2r131. |
title_full |
A subset of mouse colonic goblet cells expresses the bitter taste receptor Tas2r131. |
title_fullStr |
A subset of mouse colonic goblet cells expresses the bitter taste receptor Tas2r131. |
title_full_unstemmed |
A subset of mouse colonic goblet cells expresses the bitter taste receptor Tas2r131. |
title_sort |
subset of mouse colonic goblet cells expresses the bitter taste receptor tas2r131. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
The concept that gut nutrient sensing involves taste receptors has been fueled by recent reports associating the expression of taste receptors and taste-associated signaling molecules in the gut and in gut-derived cell lines with physiological responses induced by known taste stimuli. However, for bitter taste receptors (Tas2rs), direct evidence for their functional role in gut physiology is scarce and their cellular expression pattern remained unknown. We therefore investigated Tas2r expression in mice. RT-PCR experiments assessed the presence of mRNA for Tas2rs and taste signaling molecules in the gut. A gene-targeted mouse strain was established to visualize and identify cell types expressing the bitter receptor Tas2r131. Messenger RNA for various Tas2rs and taste signaling molecules were detected by RT-PCR in the gut. Using our knock-in mouse strain we demonstrate that a subset of colonic goblet cells express Tas2r131. Cells that express this receptor are absent in the upper gut and do not correspond to enteroendocrine and brush cells. Expression in colonic goblet cells is consistent with a role of Tas2rs in defense mechanisms against potentially harmful xenobiotics. |
url |
http://europepmc.org/articles/PMC3867391?pdf=render |
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