Obtaining of a rapid diagnostic test for Cholera, based on latex particles coupled with a monoclonal antibody against Vibrio cholera O1 lipopolysaccharide
Cholera is an acute contagious intestinal disease caused by ingestion of food or water contaminated with O1 and O139 serotypes of the bacterium Vibrio cholerae. Cholera is characterized by abundant secretory diarrhea leading to dehydration. Death occurs within hours without treatment, so early diagn...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Finlay Ediciones
2015-08-01
|
Series: | VacciMonitor |
Subjects: | |
Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2015000100003&lng=es&nrm=iso |
id |
doaj-af0b8e5b532f450bbb241d3e7f4bc787 |
---|---|
record_format |
Article |
spelling |
doaj-af0b8e5b532f450bbb241d3e7f4bc7872020-11-25T01:43:05ZengFinlay EdicionesVacciMonitor1025-02982015-08-012411020Obtaining of a rapid diagnostic test for Cholera, based on latex particles coupled with a monoclonal antibody against Vibrio cholera O1 lipopolysaccharideFátima Reyes-López0Frank Camacho-Casanova1Giovanni Duranti2Marta María Morales-Leslie3Regla Zenaida Mora-Guerra4Sonsire Fernández-Castillo5Elisabeth González-Aznar 6Claudio Piermatte 7Daniel Tomás Cardoso-González8Luis Guillermo García-Imías9Instituto Finlay. La Habana, CubaInstituto Finlay. La Habana, CubaCPM scientific , ItalyCentro Provincial de Higiene y Epidemiologia de La Habana, La Habana, CubaCentro Provincial de Higiene y Epidemiologia de La Habana, La Habana, CubaInstituto Finlay. La Habana, CubaInstituto Finlay. La Habana, CubaCPM scientific , ItalyInstituto Finlay. La Habana, CubaInstituto Finlay. La Habana, CubaCholera is an acute contagious intestinal disease caused by ingestion of food or water contaminated with O1 and O139 serotypes of the bacterium Vibrio cholerae. Cholera is characterized by abundant secretory diarrhea leading to dehydration. Death occurs within hours without treatment, so early diagnosis is very important, especially at the beginning of the disease, because it is difficult to differentiate from other acute diarrheal diseases. The diagnostic golden test is the stool culture; however, it does not guarantee a rapid detection of the disease. Rapid tests have been recently developed; they are based on test strips and agglutination with latex particles, which are very effective, but difficult to acquire for their high prices. The objective of this research was to obtain a quick assay based on latex particles coupled with a monoclonal antibody (mAb) against V. cholerae O1 lipopolysaccharide obtained in Finlay Institute. Latex particles of 0.8 µm were used in a 10% suspension, and they were coupled to the mAb (0.25 mg/ml) for 2 hours at 37°C. The sensitivity, specificity and performance were evaluated in 84 stool samples from patients with presumptive diagnosis of cholera. The diagnostic test obtained showed no cross-reactivity against no-O1 strains and other enteropathogens. Latex diagnostic test showed values of sensitivity, specificity and efficacy of 97.87; 97.29 and 97.6% respectively, very similar to the commercial diagnostic test CTK- Biotech. The latex reagent obtained can be used in the rapid diagnosis of the disease. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2015000100003&lng=es&nrm=isomonoclonal antibodies aVibrio cholerae O1 adiagnostic alatex |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fátima Reyes-López Frank Camacho-Casanova Giovanni Duranti Marta María Morales-Leslie Regla Zenaida Mora-Guerra Sonsire Fernández-Castillo Elisabeth González-Aznar Claudio Piermatte Daniel Tomás Cardoso-González Luis Guillermo García-Imías |
spellingShingle |
Fátima Reyes-López Frank Camacho-Casanova Giovanni Duranti Marta María Morales-Leslie Regla Zenaida Mora-Guerra Sonsire Fernández-Castillo Elisabeth González-Aznar Claudio Piermatte Daniel Tomás Cardoso-González Luis Guillermo García-Imías Obtaining of a rapid diagnostic test for Cholera, based on latex particles coupled with a monoclonal antibody against Vibrio cholera O1 lipopolysaccharide VacciMonitor monoclonal antibodies a Vibrio cholerae O1 a diagnostic a latex |
author_facet |
Fátima Reyes-López Frank Camacho-Casanova Giovanni Duranti Marta María Morales-Leslie Regla Zenaida Mora-Guerra Sonsire Fernández-Castillo Elisabeth González-Aznar Claudio Piermatte Daniel Tomás Cardoso-González Luis Guillermo García-Imías |
author_sort |
Fátima Reyes-López |
title |
Obtaining of a rapid diagnostic test for Cholera, based on latex particles coupled with a monoclonal antibody against Vibrio cholera O1 lipopolysaccharide |
title_short |
Obtaining of a rapid diagnostic test for Cholera, based on latex particles coupled with a monoclonal antibody against Vibrio cholera O1 lipopolysaccharide |
title_full |
Obtaining of a rapid diagnostic test for Cholera, based on latex particles coupled with a monoclonal antibody against Vibrio cholera O1 lipopolysaccharide |
title_fullStr |
Obtaining of a rapid diagnostic test for Cholera, based on latex particles coupled with a monoclonal antibody against Vibrio cholera O1 lipopolysaccharide |
title_full_unstemmed |
Obtaining of a rapid diagnostic test for Cholera, based on latex particles coupled with a monoclonal antibody against Vibrio cholera O1 lipopolysaccharide |
title_sort |
obtaining of a rapid diagnostic test for cholera, based on latex particles coupled with a monoclonal antibody against vibrio cholera o1 lipopolysaccharide |
publisher |
Finlay Ediciones |
series |
VacciMonitor |
issn |
1025-0298 |
publishDate |
2015-08-01 |
description |
Cholera is an acute contagious intestinal disease caused by ingestion of food or water contaminated with O1 and O139 serotypes of the bacterium Vibrio cholerae. Cholera is characterized by abundant secretory diarrhea leading to dehydration. Death occurs within hours without treatment, so early diagnosis is very important, especially at the beginning of the disease, because it is difficult to differentiate from other acute diarrheal diseases. The diagnostic golden test is the stool culture; however, it does not guarantee a rapid detection of the disease. Rapid tests have been recently developed; they are based on test strips and agglutination with latex particles, which are very effective, but difficult to acquire for their high prices. The objective of this research was to obtain a quick assay based on latex particles coupled with a monoclonal antibody (mAb) against V. cholerae O1 lipopolysaccharide obtained in Finlay Institute. Latex particles of 0.8 µm were used in a 10% suspension, and they were coupled to the mAb (0.25 mg/ml) for 2 hours at 37°C. The sensitivity, specificity and performance were evaluated in 84 stool samples from patients with presumptive diagnosis of cholera. The diagnostic test obtained showed no cross-reactivity against no-O1 strains and other enteropathogens. Latex diagnostic test showed values of sensitivity, specificity and efficacy of 97.87; 97.29 and 97.6% respectively, very similar to the commercial diagnostic test CTK- Biotech. The latex reagent obtained can be used in the rapid diagnosis of the disease.
|
topic |
monoclonal antibodies a Vibrio cholerae O1 a diagnostic a latex |
url |
http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2015000100003&lng=es&nrm=iso |
work_keys_str_mv |
AT fatimareyeslopez obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide AT frankcamachocasanova obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide AT giovanniduranti obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide AT martamariamoralesleslie obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide AT reglazenaidamoraguerra obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide AT sonsirefernandezcastillo obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide AT elisabethgonzalezaznar obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide AT claudiopiermatte obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide AT danieltomascardosogonzalez obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide AT luisguillermogarciaimias obtainingofarapiddiagnostictestforcholerabasedonlatexparticlescoupledwithamonoclonalantibodyagainstvibriocholerao1lipopolysaccharide |
_version_ |
1725033470959288320 |