DHCR7 rs12785878 T>C Polymorphism Is Associated With an Increased Risk of Early Onset of Alzheimer's Disease in Chinese Population
Background: Vitamin D insufficiency has been considered a risk factor for Alzheimer's disease (AD) in several studies. Recently, four single-nucleotide polymorphisms (SNPs) to be genome-wide significant for 25-hydroxyvitamin D [25(OH)D] were identified to have an association with the risk of AD...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-02-01
|
Series: | Frontiers in Genetics |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.583695/full |
id |
doaj-af0fc9409f684ac7966d78a032ca8d0e |
---|---|
record_format |
Article |
spelling |
doaj-af0fc9409f684ac7966d78a032ca8d0e2021-02-22T14:53:38ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-02-011210.3389/fgene.2021.583695583695DHCR7 rs12785878 T>C Polymorphism Is Associated With an Increased Risk of Early Onset of Alzheimer's Disease in Chinese PopulationXixi Liu0Pengfei Wu1Lu Shen2Lu Shen3Lu Shen4Lu Shen5Bin Jiao6Bin Jiao7Xinxin Liao8Haochen Wang9Jiangnan Peng10Zhangyuan Lin11Zhangyuan Lin12Department of Neurology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, ChinaDepartment of Neurology, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Diseases, Changsha, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, ChinaDepartment of Neurology, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, ChinaDepartment of Geriatrics Neurology, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopedics, Xiangya Hospital, Central South University, Changsha, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, ChinaDepartment of Orthopedics, Xiangya Hospital, Central South University, Changsha, ChinaBackground: Vitamin D insufficiency has been considered a risk factor for Alzheimer's disease (AD) in several studies. Recently, four single-nucleotide polymorphisms (SNPs) to be genome-wide significant for 25-hydroxyvitamin D [25(OH)D] were identified to have an association with the risk of AD. These include GC rs2282679 A>C, CYP2R1 rs10741657 T>C, DHCR7 rs12785878 T>C, and CYP24A1 rs6013897 T>A. However, the association between these polymorphisms and AD susceptibility in the Chinese population remains unclear.Methods: A case-control cohort study was conducted in 676 AD patients (mean age at onset was 69.52 ± 10.90 years, male: 39.2%) and 551 healthy controls (mean age was 67.73 ± 6.02 years, male: 44.8%). Genotyping was determined by PCR and SNaPshot sequencing. To determine whether the four SNPs account for risks in AD in Chinese population, multivariate logistic regression models were performed. Stratified analysis was performed based on gender and age of onset of AD, separately. Statistical significance was set at 0.0125 (0.05/4) based on Bonferroni correction.Findings:DHCR7 rs12785878 T>C was found to be significantly associated with an increased risk of early-onset Alzheimer's disease (EOAD) (n = 300, risk allele C, adjusted OR = 1.542, adjusted 95% CI = 1.176–2.023, p = 0.002). There was no statistical significance of the other three SNPs between the two groups.Interpretation: Our results suggested that DHCR7 rs12785878 T>C might be associated with an increased risk of EOAD in the Chinese population, while other polymorphisms related to vitamin D insufficiency might not be. However, due to the limited data in this study, replication studies in a larger sample are required.https://www.frontiersin.org/articles/10.3389/fgene.2021.583695/fullvitamin D insufficiencyAlzheimer's diseasesingle neucleotide polymorphismgenetic association analysis25(OH)D |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xixi Liu Pengfei Wu Lu Shen Lu Shen Lu Shen Lu Shen Bin Jiao Bin Jiao Xinxin Liao Haochen Wang Jiangnan Peng Zhangyuan Lin Zhangyuan Lin |
spellingShingle |
Xixi Liu Pengfei Wu Lu Shen Lu Shen Lu Shen Lu Shen Bin Jiao Bin Jiao Xinxin Liao Haochen Wang Jiangnan Peng Zhangyuan Lin Zhangyuan Lin DHCR7 rs12785878 T>C Polymorphism Is Associated With an Increased Risk of Early Onset of Alzheimer's Disease in Chinese Population Frontiers in Genetics vitamin D insufficiency Alzheimer's disease single neucleotide polymorphism genetic association analysis 25(OH)D |
author_facet |
Xixi Liu Pengfei Wu Lu Shen Lu Shen Lu Shen Lu Shen Bin Jiao Bin Jiao Xinxin Liao Haochen Wang Jiangnan Peng Zhangyuan Lin Zhangyuan Lin |
author_sort |
Xixi Liu |
title |
DHCR7 rs12785878 T>C Polymorphism Is Associated With an Increased Risk of Early Onset of Alzheimer's Disease in Chinese Population |
title_short |
DHCR7 rs12785878 T>C Polymorphism Is Associated With an Increased Risk of Early Onset of Alzheimer's Disease in Chinese Population |
title_full |
DHCR7 rs12785878 T>C Polymorphism Is Associated With an Increased Risk of Early Onset of Alzheimer's Disease in Chinese Population |
title_fullStr |
DHCR7 rs12785878 T>C Polymorphism Is Associated With an Increased Risk of Early Onset of Alzheimer's Disease in Chinese Population |
title_full_unstemmed |
DHCR7 rs12785878 T>C Polymorphism Is Associated With an Increased Risk of Early Onset of Alzheimer's Disease in Chinese Population |
title_sort |
dhcr7 rs12785878 t>c polymorphism is associated with an increased risk of early onset of alzheimer's disease in chinese population |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2021-02-01 |
description |
Background: Vitamin D insufficiency has been considered a risk factor for Alzheimer's disease (AD) in several studies. Recently, four single-nucleotide polymorphisms (SNPs) to be genome-wide significant for 25-hydroxyvitamin D [25(OH)D] were identified to have an association with the risk of AD. These include GC rs2282679 A>C, CYP2R1 rs10741657 T>C, DHCR7 rs12785878 T>C, and CYP24A1 rs6013897 T>A. However, the association between these polymorphisms and AD susceptibility in the Chinese population remains unclear.Methods: A case-control cohort study was conducted in 676 AD patients (mean age at onset was 69.52 ± 10.90 years, male: 39.2%) and 551 healthy controls (mean age was 67.73 ± 6.02 years, male: 44.8%). Genotyping was determined by PCR and SNaPshot sequencing. To determine whether the four SNPs account for risks in AD in Chinese population, multivariate logistic regression models were performed. Stratified analysis was performed based on gender and age of onset of AD, separately. Statistical significance was set at 0.0125 (0.05/4) based on Bonferroni correction.Findings:DHCR7 rs12785878 T>C was found to be significantly associated with an increased risk of early-onset Alzheimer's disease (EOAD) (n = 300, risk allele C, adjusted OR = 1.542, adjusted 95% CI = 1.176–2.023, p = 0.002). There was no statistical significance of the other three SNPs between the two groups.Interpretation: Our results suggested that DHCR7 rs12785878 T>C might be associated with an increased risk of EOAD in the Chinese population, while other polymorphisms related to vitamin D insufficiency might not be. However, due to the limited data in this study, replication studies in a larger sample are required. |
topic |
vitamin D insufficiency Alzheimer's disease single neucleotide polymorphism genetic association analysis 25(OH)D |
url |
https://www.frontiersin.org/articles/10.3389/fgene.2021.583695/full |
work_keys_str_mv |
AT xixiliu dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT pengfeiwu dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT lushen dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT lushen dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT lushen dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT lushen dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT binjiao dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT binjiao dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT xinxinliao dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT haochenwang dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT jiangnanpeng dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT zhangyuanlin dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation AT zhangyuanlin dhcr7rs12785878tcpolymorphismisassociatedwithanincreasedriskofearlyonsetofalzheimersdiseaseinchinesepopulation |
_version_ |
1724256682265018368 |