Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma
There is evidence that high circulating levels of IL-6 and IL-8 are markers of a poor prognosis in various types of cancer, including NB. The participation of these cytokines in the tumor microenvironment has been described to promote progression and metastasis. Our objective was to evaluate the pro...
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doaj-af21055503e941babadfc84e241341b22021-01-31T00:03:43ZengMDPI AGCancers2072-66942021-01-011352952910.3390/cancers13030529Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with NeuroblastomaSilvia Selene Moreno-Guerrero0Arturo Ramírez-Pacheco1Luz María Rocha-Ramírez2Gabriela Hernández-Pliego3Pilar Eguía-Aguilar4María Argelia Escobar-Sánchez5Alfonso Reyes-López6Luis Enrique Juárez-Villegas7Juan José Luis Sienra-Monge8Departamento de Hemato-Oncología, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación Cuauhtémoc, Ciudad de Mexico 06720, MexicoDepartamento de Hemato-Oncología, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación Cuauhtémoc, Ciudad de Mexico 06720, MexicoUnidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación, Cuauhtémoc, Ciudad de Mexico 06720, MexicoDepartamento de Hemato-Oncología, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación Cuauhtémoc, Ciudad de Mexico 06720, MexicoLaboratorio de Biología Molecular, Departamento de Patología Clínica y Experimental, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación Cuauhtémoc, Ciudad de Mexico 06720, MexicoDepartamento de Patología Clínica y Experimental, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación Cuauhtémoc, Ciudad de Mexico 06720, MexicoCentro de Estudios Económicos y Sociales en Salud, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación Cuauhtémoc, Ciudad de Mexico 06720, MexicoDepartamento de Hemato-Oncología, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación Cuauhtémoc, Ciudad de Mexico 06720, MexicoSubdirección de Pediatría Ambulatoria Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación Cuauhtémoc, Ciudad de Mexico 06720, MexicoThere is evidence that high circulating levels of IL-6 and IL-8 are markers of a poor prognosis in various types of cancer, including NB. The participation of these cytokines in the tumor microenvironment has been described to promote progression and metastasis. Our objective was to evaluate the prognostic role of genetic polymorphisms and serum levels of IL-6 and IL-8 in a cohort of Mexican pediatric patients with NB. The detection of the SNPs rs1800795 IL-6 and rs4073 and rs2227306 IL-8 was carried out by PCR-RFLP and the levels of cytokines were determined by the ELISA method. We found elevated circulating levels of IL-8 and IL-6 in NB patients compared to the control group. The genotype frequencies of the rs1800795 IL-6 and rs4073 IL-8 variants were different between the patients with NB and the control group. Likewise, the survival analysis showed that the GG genotypes of rs1800795 IL-6 (<i>p</i> = 0.014) and AA genotypes of rs4073 IL-8 (<i>p</i> = 0.002), as well as high levels of IL-6 (<i>p</i> = 0.009) and IL-8 (<i>p</i> = 0.046), were associated with lower overall survival. We confirmed the impact on an adverse prognosis in a multivariate model. This study suggests that the SNPs rs1800795 IL-6 and rs4073 IL-8 and their serum levels could be promising biomarkers of a poor prognosis, associated with overall survival, metastasis, and a high risk in Mexican children with NB.https://www.mdpi.com/2072-6694/13/3/529neuroblastomainterleukin-6interleukin-8single nucleotide polymorphismoverall survivalprognostic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Silvia Selene Moreno-Guerrero Arturo Ramírez-Pacheco Luz María Rocha-Ramírez Gabriela Hernández-Pliego Pilar Eguía-Aguilar María Argelia Escobar-Sánchez Alfonso Reyes-López Luis Enrique Juárez-Villegas Juan José Luis Sienra-Monge |
spellingShingle |
Silvia Selene Moreno-Guerrero Arturo Ramírez-Pacheco Luz María Rocha-Ramírez Gabriela Hernández-Pliego Pilar Eguía-Aguilar María Argelia Escobar-Sánchez Alfonso Reyes-López Luis Enrique Juárez-Villegas Juan José Luis Sienra-Monge Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma Cancers neuroblastoma interleukin-6 interleukin-8 single nucleotide polymorphism overall survival prognostic |
author_facet |
Silvia Selene Moreno-Guerrero Arturo Ramírez-Pacheco Luz María Rocha-Ramírez Gabriela Hernández-Pliego Pilar Eguía-Aguilar María Argelia Escobar-Sánchez Alfonso Reyes-López Luis Enrique Juárez-Villegas Juan José Luis Sienra-Monge |
author_sort |
Silvia Selene Moreno-Guerrero |
title |
Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma |
title_short |
Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma |
title_full |
Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma |
title_fullStr |
Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma |
title_full_unstemmed |
Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma |
title_sort |
association of genetic polymorphisms and serum levels of il-6 and il-8 with the prognosis in children with neuroblastoma |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2021-01-01 |
description |
There is evidence that high circulating levels of IL-6 and IL-8 are markers of a poor prognosis in various types of cancer, including NB. The participation of these cytokines in the tumor microenvironment has been described to promote progression and metastasis. Our objective was to evaluate the prognostic role of genetic polymorphisms and serum levels of IL-6 and IL-8 in a cohort of Mexican pediatric patients with NB. The detection of the SNPs rs1800795 IL-6 and rs4073 and rs2227306 IL-8 was carried out by PCR-RFLP and the levels of cytokines were determined by the ELISA method. We found elevated circulating levels of IL-8 and IL-6 in NB patients compared to the control group. The genotype frequencies of the rs1800795 IL-6 and rs4073 IL-8 variants were different between the patients with NB and the control group. Likewise, the survival analysis showed that the GG genotypes of rs1800795 IL-6 (<i>p</i> = 0.014) and AA genotypes of rs4073 IL-8 (<i>p</i> = 0.002), as well as high levels of IL-6 (<i>p</i> = 0.009) and IL-8 (<i>p</i> = 0.046), were associated with lower overall survival. We confirmed the impact on an adverse prognosis in a multivariate model. This study suggests that the SNPs rs1800795 IL-6 and rs4073 IL-8 and their serum levels could be promising biomarkers of a poor prognosis, associated with overall survival, metastasis, and a high risk in Mexican children with NB. |
topic |
neuroblastoma interleukin-6 interleukin-8 single nucleotide polymorphism overall survival prognostic |
url |
https://www.mdpi.com/2072-6694/13/3/529 |
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