Frequency of serine protease autotransporters of Enterobacteriaceae (SPATE) encoding genes in diffusely adherent Escherichia coli (DAEC) isolates from children with and without diarrhea
The aim of the study was to determine the frequency of six genes encoding serine protease autotransporter proteins Enterobacteriaceae (SPATE) in diffusely adherent Escherichia coli (DAEC) isolates from children with (WD, n=63) and without diarrhea (WOD, n=41) from Lima, Peru. WOD were considered a c...
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doaj-af30d1b2adc549b6a27872cf6b62a8142021-05-11T21:46:26ZspaInstituto Nacional de SaludRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422021-03-01381124910.17843/rpmesp.2021.381.57502478Frequency of serine protease autotransporters of Enterobacteriaceae (SPATE) encoding genes in diffusely adherent Escherichia coli (DAEC) isolates from children with and without diarrheaKaren Ocampo0Maribel Riveros1Maria Pinedo-Bardales2Joaquim Ruiz3Theresa J. Ochoa4Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú; Universidad Nacional Federico Villarreal, Lima, Perú.Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú; Universidad Nacional Federico Villarreal, Lima, Perú.Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú; Facultad de Ciencias, Universidad Nacional de Piura, Piura, PerúISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, España; Laboratorio de Microbiología Molecular y Genómica Bacteriana, Universidad Científica del Sur, Lima, Perú.Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú; School of Public Health, University of Texas Health Science Center at Houston, Houston, Estados Unidos.The aim of the study was to determine the frequency of six genes encoding serine protease autotransporter proteins Enterobacteriaceae (SPATE) in diffusely adherent Escherichia coli (DAEC) isolates from children with (WD, n=63) and without diarrhea (WOD, n=41) from Lima, Peru. WOD were considered a control group. For the detection of the genes, 2 multiple PCRs were standardized: triple A (sigA, pet, espP) and triple B (sat, pic, espC). In both groups, the most frequent SPATE gene was Sat (39.7% of WD and 41.5% of WOD), followed by spP (20.6% and 9.7% in WD and WOD respectively). The other genes were detected in proportions lower than 10.0%, in the following order of frequency: pet, sigA, espC and pic, without significant differences between the groups. It was concluded that Sat is the most frequent SPATE in DAEC and that these strains may possess SPATE genes regardless of whether they are isolated in WD or WOD.https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/5750escherichia coli de adherencia difusafactores de virulenciadiarreaniño |
collection |
DOAJ |
language |
Spanish |
format |
Article |
sources |
DOAJ |
author |
Karen Ocampo Maribel Riveros Maria Pinedo-Bardales Joaquim Ruiz Theresa J. Ochoa |
spellingShingle |
Karen Ocampo Maribel Riveros Maria Pinedo-Bardales Joaquim Ruiz Theresa J. Ochoa Frequency of serine protease autotransporters of Enterobacteriaceae (SPATE) encoding genes in diffusely adherent Escherichia coli (DAEC) isolates from children with and without diarrhea Revista Peruana de Medicina Experimental y Salud Pública escherichia coli de adherencia difusa factores de virulencia diarrea niño |
author_facet |
Karen Ocampo Maribel Riveros Maria Pinedo-Bardales Joaquim Ruiz Theresa J. Ochoa |
author_sort |
Karen Ocampo |
title |
Frequency of serine protease autotransporters of Enterobacteriaceae (SPATE) encoding genes in diffusely adherent Escherichia coli (DAEC) isolates from children with and without diarrhea |
title_short |
Frequency of serine protease autotransporters of Enterobacteriaceae (SPATE) encoding genes in diffusely adherent Escherichia coli (DAEC) isolates from children with and without diarrhea |
title_full |
Frequency of serine protease autotransporters of Enterobacteriaceae (SPATE) encoding genes in diffusely adherent Escherichia coli (DAEC) isolates from children with and without diarrhea |
title_fullStr |
Frequency of serine protease autotransporters of Enterobacteriaceae (SPATE) encoding genes in diffusely adherent Escherichia coli (DAEC) isolates from children with and without diarrhea |
title_full_unstemmed |
Frequency of serine protease autotransporters of Enterobacteriaceae (SPATE) encoding genes in diffusely adherent Escherichia coli (DAEC) isolates from children with and without diarrhea |
title_sort |
frequency of serine protease autotransporters of enterobacteriaceae (spate) encoding genes in diffusely adherent escherichia coli (daec) isolates from children with and without diarrhea |
publisher |
Instituto Nacional de Salud |
series |
Revista Peruana de Medicina Experimental y Salud Pública |
issn |
1726-4634 1726-4642 |
publishDate |
2021-03-01 |
description |
The aim of the study was to determine the frequency of six genes encoding serine protease autotransporter proteins Enterobacteriaceae (SPATE) in diffusely adherent Escherichia coli (DAEC) isolates from children with (WD, n=63) and without diarrhea (WOD, n=41) from Lima, Peru. WOD were considered a control group. For the detection of the genes, 2 multiple PCRs were standardized: triple A (sigA, pet, espP) and triple B (sat, pic, espC). In both groups, the most frequent SPATE gene was Sat (39.7% of WD and 41.5% of WOD), followed by spP (20.6% and 9.7% in WD and WOD respectively). The other genes were detected in proportions lower than 10.0%, in the following order of frequency: pet, sigA, espC and pic, without significant differences between the groups. It was concluded that Sat is the most frequent SPATE in DAEC and that these strains may possess SPATE genes regardless of whether they are isolated in WD or WOD. |
topic |
escherichia coli de adherencia difusa factores de virulencia diarrea niño |
url |
https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/5750 |
work_keys_str_mv |
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