Mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cells
<p>Abstract</p> <p>Background</p> <p>Mannose binding lectin (MBL) is an important host defence protein against opportunistic fungal pathogens. This carbohydrate-binding protein, an opsonin and lectin pathway activator, binds through multiple lectin domains to the repeat...
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doaj-af4ab5b934af4a0ead2324f96bd510722020-11-25T02:35:56ZengBMCBMC Microbiology1471-21802008-12-018122910.1186/1471-2180-8-229Mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cellsHerpers Bjorn LScharringa JelleHoepelman Andy IMvan Asbeck Eveline CVerhoef Jan<p>Abstract</p> <p>Background</p> <p>Mannose binding lectin (MBL) is an important host defence protein against opportunistic fungal pathogens. This carbohydrate-binding protein, an opsonin and lectin pathway activator, binds through multiple lectin domains to the repeating sugar arrays displayed on the surface of a wide range of clinically relevant microbial species. We investigated the contribution of MBL to antifungal innate immunity towards <it>C. parapsilosis in vitro</it>.</p> <p>Results</p> <p>High avidity binding was observed between MBL and <it>C. albicans </it>and <it>C. parapsilosis</it>. Addition of MBL to MBL deficient serum increased the deposition of C4 and C3b and enhanced the uptake of <it>C. albicans</it>, <it>C. parapsilosis </it>and acapsular <it>C. neoformans </it>by polymorphonuclear cells (PMNs). Compared to other microorganisms, such as <it>Escherichia coli</it>, <it>Staphylococcus aureus </it>and <it>Cryptococcus neoformans</it>, <it>C. parapsilosis </it>and <it>Candida albicans </it>were potent activators of the lectin pathway.</p> <p>Conclusion</p> <p>Our results suggest that MBL plays a crucial role in the innate immunity against infections caused by yeast by increasing uptake by PMN.</p> http://www.biomedcentral.com/1471-2180/8/229 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Herpers Bjorn L Scharringa Jelle Hoepelman Andy IM van Asbeck Eveline C Verhoef Jan |
spellingShingle |
Herpers Bjorn L Scharringa Jelle Hoepelman Andy IM van Asbeck Eveline C Verhoef Jan Mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cells BMC Microbiology |
author_facet |
Herpers Bjorn L Scharringa Jelle Hoepelman Andy IM van Asbeck Eveline C Verhoef Jan |
author_sort |
Herpers Bjorn L |
title |
Mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cells |
title_short |
Mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cells |
title_full |
Mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cells |
title_fullStr |
Mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cells |
title_full_unstemmed |
Mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cells |
title_sort |
mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cells |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2008-12-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Mannose binding lectin (MBL) is an important host defence protein against opportunistic fungal pathogens. This carbohydrate-binding protein, an opsonin and lectin pathway activator, binds through multiple lectin domains to the repeating sugar arrays displayed on the surface of a wide range of clinically relevant microbial species. We investigated the contribution of MBL to antifungal innate immunity towards <it>C. parapsilosis in vitro</it>.</p> <p>Results</p> <p>High avidity binding was observed between MBL and <it>C. albicans </it>and <it>C. parapsilosis</it>. Addition of MBL to MBL deficient serum increased the deposition of C4 and C3b and enhanced the uptake of <it>C. albicans</it>, <it>C. parapsilosis </it>and acapsular <it>C. neoformans </it>by polymorphonuclear cells (PMNs). Compared to other microorganisms, such as <it>Escherichia coli</it>, <it>Staphylococcus aureus </it>and <it>Cryptococcus neoformans</it>, <it>C. parapsilosis </it>and <it>Candida albicans </it>were potent activators of the lectin pathway.</p> <p>Conclusion</p> <p>Our results suggest that MBL plays a crucial role in the innate immunity against infections caused by yeast by increasing uptake by PMN.</p> |
url |
http://www.biomedcentral.com/1471-2180/8/229 |
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