Treatment with Bifidobacteria can suppress Aβ accumulation and neuroinflammation in APP/PS1 mice
Background Alzheimer’s disease (AD), being a complex disorder, is affected either by genetic or environmental factors or both. It is observed that there is an excessive accumulation of amyloid β (Aβ) in the extracellular space of the brain. AD is the first neurodegenerative disease in the elderly, a...
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doaj-af64fcef1f384ee59b3056713c7021bb2020-11-25T03:41:14ZengPeerJ Inc.PeerJ2167-83592020-10-018e1026210.7717/peerj.10262Treatment with Bifidobacteria can suppress Aβ accumulation and neuroinflammation in APP/PS1 miceQiong Wu0Qifa Li1Xuan Zhang2Michael Ntim3Xuefei Wu4Ming Li5Li Wang6Jie Zhao7Shao Li8Liaoning Provincial Key Laboratory of Cerebral Diseases in Department of Physiology, Dalian Medical University, Dalian, ChinaFunctional Laboratory, Dalian Medical University, Dalian, ChinaNational-Local Joint Engineering Research Center for Drug-Research and Development (R & D) of Neurodegenerative Diseases, Dalian Medical University, Dalian, ChinaLiaoning Provincial Key Laboratory of Cerebral Diseases in Department of Physiology, Dalian Medical University, Dalian, ChinaLiaoning Provincial Key Laboratory of Cerebral Diseases in Department of Physiology, Dalian Medical University, Dalian, ChinaDepartment of Microecology, Dalian Medical University, Dalian, ChinaCollege of Pharmacy, Dalian Medical University, Dalian, ChinaNational-Local Joint Engineering Research Center for Drug-Research and Development (R & D) of Neurodegenerative Diseases, Dalian Medical University, Dalian, ChinaLiaoning Provincial Key Laboratory of Cerebral Diseases in Department of Physiology, Dalian Medical University, Dalian, ChinaBackground Alzheimer’s disease (AD), being a complex disorder, is affected either by genetic or environmental factors or both. It is observed that there is an excessive accumulation of amyloid β (Aβ) in the extracellular space of the brain. AD is the first neurodegenerative disease in the elderly, and so far there is no effective treatment. In recent years, many studies have reported that Alzheimer’s disease has a relationship with gut microflora, indicating that regulating gut microbiota could offer therapeutic intervention for AD. This study explored the effect Bifidobacteria has in averting AD. Methods WT and APP/PS1 mice were used for the experiments. The mice were randomly assigned to four groups: WT group, WT + Bi group, AD group (APP/PS1 mouse) and AD + Bi group (Bifidobacteria-treated APP/PS1 mouse). Treatment with Bifidobacteria lasted for 6 months and mice were prepared for immunohistochemistry, immunofluorescence, Thioflavin S staining, Western blotting, PCR and Elisa quantitative assay. Results The results show that after 6 months of treatment with Bifidobacteria signiis to be lesficantly reduces Aβ deposition in cortex and hippocampus of AD mice. The level of insoluble Aβ in the hippocampus and cortex of AD+Bi mice was decreased compared with AD mice. Meanwhile, a significant decrease in the level of soluble Aβ in the cortex of AD+Bi mice but not in the hippocampus was observed. The activation of microglia and the release of inflammatory factors were also determined in this study. From the results, Bifidobacteria inhibited microglial activation and reduced IL-1β, TNF-α, IL-4, IL-6 and INF-γ release. Altogether, these results implied that Bifidobacteria can alleviate the pathological changes of AD through various effects.https://peerj.com/articles/10262.pdfBifidobacteriaADAβGut microflora |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiong Wu Qifa Li Xuan Zhang Michael Ntim Xuefei Wu Ming Li Li Wang Jie Zhao Shao Li |
spellingShingle |
Qiong Wu Qifa Li Xuan Zhang Michael Ntim Xuefei Wu Ming Li Li Wang Jie Zhao Shao Li Treatment with Bifidobacteria can suppress Aβ accumulation and neuroinflammation in APP/PS1 mice PeerJ Bifidobacteria AD Aβ Gut microflora |
author_facet |
Qiong Wu Qifa Li Xuan Zhang Michael Ntim Xuefei Wu Ming Li Li Wang Jie Zhao Shao Li |
author_sort |
Qiong Wu |
title |
Treatment with Bifidobacteria can suppress Aβ accumulation and neuroinflammation in APP/PS1 mice |
title_short |
Treatment with Bifidobacteria can suppress Aβ accumulation and neuroinflammation in APP/PS1 mice |
title_full |
Treatment with Bifidobacteria can suppress Aβ accumulation and neuroinflammation in APP/PS1 mice |
title_fullStr |
Treatment with Bifidobacteria can suppress Aβ accumulation and neuroinflammation in APP/PS1 mice |
title_full_unstemmed |
Treatment with Bifidobacteria can suppress Aβ accumulation and neuroinflammation in APP/PS1 mice |
title_sort |
treatment with bifidobacteria can suppress aβ accumulation and neuroinflammation in app/ps1 mice |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2020-10-01 |
description |
Background Alzheimer’s disease (AD), being a complex disorder, is affected either by genetic or environmental factors or both. It is observed that there is an excessive accumulation of amyloid β (Aβ) in the extracellular space of the brain. AD is the first neurodegenerative disease in the elderly, and so far there is no effective treatment. In recent years, many studies have reported that Alzheimer’s disease has a relationship with gut microflora, indicating that regulating gut microbiota could offer therapeutic intervention for AD. This study explored the effect Bifidobacteria has in averting AD. Methods WT and APP/PS1 mice were used for the experiments. The mice were randomly assigned to four groups: WT group, WT + Bi group, AD group (APP/PS1 mouse) and AD + Bi group (Bifidobacteria-treated APP/PS1 mouse). Treatment with Bifidobacteria lasted for 6 months and mice were prepared for immunohistochemistry, immunofluorescence, Thioflavin S staining, Western blotting, PCR and Elisa quantitative assay. Results The results show that after 6 months of treatment with Bifidobacteria signiis to be lesficantly reduces Aβ deposition in cortex and hippocampus of AD mice. The level of insoluble Aβ in the hippocampus and cortex of AD+Bi mice was decreased compared with AD mice. Meanwhile, a significant decrease in the level of soluble Aβ in the cortex of AD+Bi mice but not in the hippocampus was observed. The activation of microglia and the release of inflammatory factors were also determined in this study. From the results, Bifidobacteria inhibited microglial activation and reduced IL-1β, TNF-α, IL-4, IL-6 and INF-γ release. Altogether, these results implied that Bifidobacteria can alleviate the pathological changes of AD through various effects. |
topic |
Bifidobacteria AD Aβ Gut microflora |
url |
https://peerj.com/articles/10262.pdf |
work_keys_str_mv |
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