Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Pseudomonas aeruginosa isolates in Russia: results of multicenter epidemiological study “MARATHON 2015–2016”
Objective. Objectives. To assess the rates of antibiotic resistance, production of acquired carbapenemases, genotypes and prevalence of «international high-risk clones» among nosocomial strains of Pseudomonas aeruginosa isolated in various regions of Russia within the «MARATHON 2015–2016» study....
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Format: | Article |
Language: | Russian |
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Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy
2019-10-01
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Series: | Клиническая микробиология и антимикробная химиотерапия |
Subjects: | |
Online Access: | https://cmac-journal.ru/publication/2019/2/cmac-2019-t21-n2-p160/cmac-2019-t21-n2-p160.pdf |
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Russian |
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author |
Edelstein M.V. Shek E.A. Sukhorukova M.V. Skleenova E.Yu. Ivanchik N.V. Shajdullina E.R. Mikotina A.V. Kuzmenkov A.Yu. Dekhnich A.V. Kozlov R.S. Semyonova N.V. Slepakova S.A. Shepotajlova N.V. Strebkova V.V. Rybina N.A. Yaranceva N.Z. Perevalova E.Yu. Rozanova S.M. Nagovicina S.G. Moldovanu M.G. Nasybullova Z.Z. Arkhipenko M.V. Shakhmuradyan R.M. Nizhegorodceva I.A. Varibrus E.V. Aleksandrova I.A. Lazareva A.V. Kryzhanovskaya O.A. Markelova N.N. Chernyavskaya Yu.L. Lebedeva E.V. Kirillova G.Sh. Bekker G.G. Popova L.D. Elokhina E.V. Smol’kova Yu.E. Zinov’ev D.Yu. Ityaeva L.N. Blinova G.Yu. Zubareva N.A. Vityazeva V.P. Plaksina M.G. Kucevalova O.Yu. Panova N.I. Suborova T.N. Polukhina O.V. Voroshilova T.M. Churikova E.M. Moskvitina E.N. Krechikova O.I. Petrova T.A. Mart’yanova N.M. Khokhlova K.O. Gudkova L.V. Bykonya S.A. Khokhlyavina R.M. Shpil’kina L.V. Burasova E.G. Khrebtovskaya V.A. Molchanova I.V. Zvonaryova O.V. Kornilova P.A. Kryanga V.G. Portnyagina U.S. Shamaeva S.Kh. Popov D.A. Vostrikova T.Yu. |
spellingShingle |
Edelstein M.V. Shek E.A. Sukhorukova M.V. Skleenova E.Yu. Ivanchik N.V. Shajdullina E.R. Mikotina A.V. Kuzmenkov A.Yu. Dekhnich A.V. Kozlov R.S. Semyonova N.V. Slepakova S.A. Shepotajlova N.V. Strebkova V.V. Rybina N.A. Yaranceva N.Z. Perevalova E.Yu. Rozanova S.M. Nagovicina S.G. Moldovanu M.G. Nasybullova Z.Z. Arkhipenko M.V. Shakhmuradyan R.M. Nizhegorodceva I.A. Varibrus E.V. Aleksandrova I.A. Lazareva A.V. Kryzhanovskaya O.A. Markelova N.N. Chernyavskaya Yu.L. Lebedeva E.V. Kirillova G.Sh. Bekker G.G. Popova L.D. Elokhina E.V. Smol’kova Yu.E. Zinov’ev D.Yu. Ityaeva L.N. Blinova G.Yu. Zubareva N.A. Vityazeva V.P. Plaksina M.G. Kucevalova O.Yu. Panova N.I. Suborova T.N. Polukhina O.V. Voroshilova T.M. Churikova E.M. Moskvitina E.N. Krechikova O.I. Petrova T.A. Mart’yanova N.M. Khokhlova K.O. Gudkova L.V. Bykonya S.A. Khokhlyavina R.M. Shpil’kina L.V. Burasova E.G. Khrebtovskaya V.A. Molchanova I.V. Zvonaryova O.V. Kornilova P.A. Kryanga V.G. Portnyagina U.S. Shamaeva S.Kh. Popov D.A. Vostrikova T.Yu. Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Pseudomonas aeruginosa isolates in Russia: results of multicenter epidemiological study “MARATHON 2015–2016” Клиническая микробиология и антимикробная химиотерапия nosocomial infections pseudomonas aeruginosa antimicrobial resistance carbapenemases |
author_facet |
Edelstein M.V. Shek E.A. Sukhorukova M.V. Skleenova E.Yu. Ivanchik N.V. Shajdullina E.R. Mikotina A.V. Kuzmenkov A.Yu. Dekhnich A.V. Kozlov R.S. Semyonova N.V. Slepakova S.A. Shepotajlova N.V. Strebkova V.V. Rybina N.A. Yaranceva N.Z. Perevalova E.Yu. Rozanova S.M. Nagovicina S.G. Moldovanu M.G. Nasybullova Z.Z. Arkhipenko M.V. Shakhmuradyan R.M. Nizhegorodceva I.A. Varibrus E.V. Aleksandrova I.A. Lazareva A.V. Kryzhanovskaya O.A. Markelova N.N. Chernyavskaya Yu.L. Lebedeva E.V. Kirillova G.Sh. Bekker G.G. Popova L.D. Elokhina E.V. Smol’kova Yu.E. Zinov’ev D.Yu. Ityaeva L.N. Blinova G.Yu. Zubareva N.A. Vityazeva V.P. Plaksina M.G. Kucevalova O.Yu. Panova N.I. Suborova T.N. Polukhina O.V. Voroshilova T.M. Churikova E.M. Moskvitina E.N. Krechikova O.I. Petrova T.A. Mart’yanova N.M. Khokhlova K.O. Gudkova L.V. Bykonya S.A. Khokhlyavina R.M. Shpil’kina L.V. Burasova E.G. Khrebtovskaya V.A. Molchanova I.V. Zvonaryova O.V. Kornilova P.A. Kryanga V.G. Portnyagina U.S. Shamaeva S.Kh. Popov D.A. Vostrikova T.Yu. |
author_sort |
Edelstein M.V. |
title |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Pseudomonas aeruginosa isolates in Russia: results of multicenter epidemiological study “MARATHON 2015–2016” |
title_short |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Pseudomonas aeruginosa isolates in Russia: results of multicenter epidemiological study “MARATHON 2015–2016” |
title_full |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Pseudomonas aeruginosa isolates in Russia: results of multicenter epidemiological study “MARATHON 2015–2016” |
title_fullStr |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Pseudomonas aeruginosa isolates in Russia: results of multicenter epidemiological study “MARATHON 2015–2016” |
title_full_unstemmed |
Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Pseudomonas aeruginosa isolates in Russia: results of multicenter epidemiological study “MARATHON 2015–2016” |
title_sort |
antimicrobial resistance, carbapenemase production, and genotypes of nosocomial pseudomonas aeruginosa isolates in russia: results of multicenter epidemiological study “marathon 2015–2016” |
publisher |
Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy |
series |
Клиническая микробиология и антимикробная химиотерапия |
issn |
1684-4386 2686-9586 |
publishDate |
2019-10-01 |
description |
Objective.
Objectives. To assess the rates of antibiotic resistance, production of acquired carbapenemases, genotypes and prevalence of «international high-risk clones» among nosocomial strains of Pseudomonas aeruginosa isolated in various regions of Russia within the «MARATHON 2015–2016» study.
Materials and Methods.
A total of 1006 non-duplicate nosocomial isolates of P. aeruginosa collected in 44 hospitals from 25 cities in Russia in 2015–2016 were studied. Species identification of isolates was performed by means of MALDI-TOF mass-spectrometry. Antimicrobial susceptibility was determined using broth microdilution method according to ISO 20776-1:2006 and interpreted using EUCAST MIC clinical breakpoints v.9.0. The presence of acquired carbapenemase genes of VIM, IMP, NDM, GES-2 and GES-5 groups was determined using real-time PCR. Genotyping of isolates was performed by analysis of selected single nucleotide polymorphisms in 7 chromosomal loci used for multi-locus sequence typing (MLST) of this species.
Results.
P. aeruginosa comprised of 17.4% of all bacterial pathogens isolated within the «MARATHON 2015–2016» study. The resistance rates to antibiotics were (in decreasing order of in vitro activity): 1.4% to colistin, 41.5% to aztreonam, 41.6% to ceftazidime/avibactam, 47.7% to amikacin, 51.5% to cefepime, 54.2% to tobramycin, 55.5% to meropenem, 56.3% to gentamicin, 56.8% to ceftazidime, 62.0% to piperacillin/tazobactam, 63.3% to ciprofloxacin, 65.2% to piperacillin, 67.5% to imipenem, and 97,6% to ticarcillin/clavulanic acid. Acquired carbapenemase genes were detected in 35% of the isolates including those for metallo-β-lactamases of VIM- (30.5%) and IMP-type (0.3%) and for GES-5-like serine carbapenemases. MBL producers were highly resistant to all antibiotics except for aztreonam (49%) and polymyxins (0%); GES-5-producers were resistant to most antibiotics except for aztreonam (4.8%), ceftazidime/avibactam (11.9%) and polymyxins (0%). The majority of carbapenemase producing strains belonged to the international “high-risk” CC235 (76.3%) and CC654 (21%) clones. All CC235 isolates revealed the presence of specific variant of the gene encoding secreted phospholipase A2, ExoU, which is a major virulence factor of P. aeruginosa.
Conclusions.
The results of this study indicate an increase in resistance rates to most antibiotics, including carbapenems, and in prevalence of carbapenemase production in nosocomial strains of P. aeruginosa. This can be largely attributed to the expansion of “high-risk” clones. |
topic |
nosocomial infections pseudomonas aeruginosa antimicrobial resistance carbapenemases |
url |
https://cmac-journal.ru/publication/2019/2/cmac-2019-t21-n2-p160/cmac-2019-t21-n2-p160.pdf |
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doaj-af693dae08084fab94ac81d3aac349c82020-11-25T01:46:31ZrusInterregional Association for Clinical Microbiology and Antimicrobial ChemotherapyКлиническая микробиология и антимикробная химиотерапия1684-43862686-95862019-10-0121216017010.36488/cmac.2019.2.160-170cmac-2019-t21-n2-p160Antimicrobial resistance, carbapenemase production, and genotypes of nosocomial Pseudomonas aeruginosa isolates in Russia: results of multicenter epidemiological study “MARATHON 2015–2016”Edelstein M.V.0Shek E.A.1Sukhorukova M.V.2Skleenova E.Yu.3Ivanchik N.V.4Shajdullina E.R.5Mikotina A.V.6Kuzmenkov A.Yu.7Dekhnich A.V.8Kozlov R.S.9Semyonova N.V.10Slepakova S.A.11Shepotajlova N.V.12Strebkova V.V.13Rybina N.A.14Yaranceva N.Z.15Perevalova E.Yu.16Rozanova S.M.17Nagovicina S.G.18Moldovanu M.G.19Nasybullova Z.Z.20Arkhipenko M.V.21Shakhmuradyan R.M.22Nizhegorodceva I.A.23Varibrus E.V.24Aleksandrova I.A.25Lazareva A.V.26Kryzhanovskaya O.A.27Markelova N.N.28Chernyavskaya Yu.L.29Lebedeva E.V.30Kirillova G.Sh.31Bekker G.G.32Popova L.D.33Elokhina E.V.34Smol’kova Yu.E.35Zinov’ev D.Yu.36Ityaeva L.N.37Blinova G.Yu.38Zubareva N.A.39Vityazeva V.P.40Plaksina M.G.41Kucevalova O.Yu.42Panova N.I.43Suborova T.N.44Polukhina O.V.45Voroshilova T.M.46Churikova E.M.47Moskvitina E.N.48Krechikova O.I.49Petrova T.A.50Mart’yanova N.M.51Khokhlova K.O.52Gudkova L.V.53Bykonya S.A.54Khokhlyavina R.M.55Shpil’kina L.V.56Burasova E.G.57Khrebtovskaya V.A.58Molchanova I.V.59Zvonaryova O.V.60Kornilova P.A.61Kryanga V.G.62Portnyagina U.S.63Shamaeva S.Kh.64Popov D.A.65Vostrikova T.Yu.66Institute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaArkhangelsk Regional Clinical Hospital, Arkhangelsk, RussiaAmur Regional Children Clinical Hospital, Blagoveshchensk, RussiaAmur Regional Children Clinical Hospital, Blagoveshchensk, RussiaVoronezh City Clinical Emergency Care HospitalVoronezh City Clinical Emergency Care HospitalSverdlovsk Regional Clinical Psychiatric Hospital, Yekaterinburg, RussiaClinical Diagnostic Center, Yekaterinburg, RussiaClinical Diagnostic Center, Yekaterinburg, RussiaCity Clinical HospitalCity Clinical HospitalRepublican Clinical Hospital, Kazan, RussiaResearch Institute – Regional Clinical HospitalResearch Institute – Regional Clinical HospitalRegional Clinical HospitalRegional Clinical HospitalNational Medical Research Center of Neurosurgery named after N.N. Burdenko, Moscow, RussiaNational Medical Research Center for Children’s Health, Moscow, RussiaNational Medical Research Center for Children’s Health, Moscow, RussiaRussian Scientific Center of Roentgenoradiology, Moscow, RussiaMurmansk Regional Clinical Hospital named after P.A. Bayandin, Murmansk, RussiaMurmansk Regional Clinical Hospital named after P.A. Bayandin, Murmansk, RussiaEmergency Care Hospital, Naberezhnye Chelny, RussiaClinical Hospital at the Novosibirsk-Main Station, Novosibirsk, RussiaRegional Clinical Hospital, Omsk, RussiaRegional Clinical Hospital, Omsk, RussiaClinical HospitalClinical HospitalRegional Oncology Dispensary, Penza, RussiaRegional Oncology Dispensary, Penza, RussiaPerm State Medical University named after E.A. Wagner, Perm, RussiaChildren Republican Hospital, Petrozavodsk, RussiaChildren Republican Hospital, Petrozavodsk, RussiaRostov Research Institute of Oncology, Rostov-on-Don, RussiaRostov Research Institute of Oncology, Rostov-on-Don, RussiaS.M. Kirov Military Medical Academy, Saint-Petersburg, RussiaSaint-Petersburg, RussiaAll-Russian Center of Emergency and Radiation Medicine named after A.M. Nikiforov, Saint-Petersburg, RussiaAll-Russian Center of Emergency and Radiation Medicine named after A.M. Nikiforov, Saint-Petersburg, RussiaSiberian Federal Scientific Clinical Center, Seversk, RussiaInstitute of Antimicrobial Chemotherapy, Smolensk, RussiaClinical HospitalTol’yatti City Clinical HospitalTol’yatti City Clinical HospitalTomsk Regional Clinical Hospital, Tomsk, RussiaTomsk Regional Clinical Hospital, Tomsk, RussiaRegional Clinical HospitalRegional Clinical HospitalRepublican Clinical Hospital named after N.A. Semashko, Ulan-Ude, RussiaRepublican Clinical Hospital named after N.A. Semashko, Ulan-Ude, RussiaChelyabinsk Regional Clinical Hospital, Chelyabinsk, RussiaChelyabinsk Regional Clinical Hospital, Chelyabinsk, RussiaRepublican HospitalRepublican HospitalRepublican HospitalRepublican HospitalA.N. Bakulev National Medical Research Center of Cardiovascular Surgery, Moscow, RussiaA.N. Bakulev National Medical Research Center of Cardiovascular Surgery, Moscow, RussiaObjective. Objectives. To assess the rates of antibiotic resistance, production of acquired carbapenemases, genotypes and prevalence of «international high-risk clones» among nosocomial strains of Pseudomonas aeruginosa isolated in various regions of Russia within the «MARATHON 2015–2016» study. Materials and Methods. A total of 1006 non-duplicate nosocomial isolates of P. aeruginosa collected in 44 hospitals from 25 cities in Russia in 2015–2016 were studied. Species identification of isolates was performed by means of MALDI-TOF mass-spectrometry. Antimicrobial susceptibility was determined using broth microdilution method according to ISO 20776-1:2006 and interpreted using EUCAST MIC clinical breakpoints v.9.0. The presence of acquired carbapenemase genes of VIM, IMP, NDM, GES-2 and GES-5 groups was determined using real-time PCR. Genotyping of isolates was performed by analysis of selected single nucleotide polymorphisms in 7 chromosomal loci used for multi-locus sequence typing (MLST) of this species. Results. P. aeruginosa comprised of 17.4% of all bacterial pathogens isolated within the «MARATHON 2015–2016» study. The resistance rates to antibiotics were (in decreasing order of in vitro activity): 1.4% to colistin, 41.5% to aztreonam, 41.6% to ceftazidime/avibactam, 47.7% to amikacin, 51.5% to cefepime, 54.2% to tobramycin, 55.5% to meropenem, 56.3% to gentamicin, 56.8% to ceftazidime, 62.0% to piperacillin/tazobactam, 63.3% to ciprofloxacin, 65.2% to piperacillin, 67.5% to imipenem, and 97,6% to ticarcillin/clavulanic acid. Acquired carbapenemase genes were detected in 35% of the isolates including those for metallo-β-lactamases of VIM- (30.5%) and IMP-type (0.3%) and for GES-5-like serine carbapenemases. MBL producers were highly resistant to all antibiotics except for aztreonam (49%) and polymyxins (0%); GES-5-producers were resistant to most antibiotics except for aztreonam (4.8%), ceftazidime/avibactam (11.9%) and polymyxins (0%). The majority of carbapenemase producing strains belonged to the international “high-risk” CC235 (76.3%) and CC654 (21%) clones. All CC235 isolates revealed the presence of specific variant of the gene encoding secreted phospholipase A2, ExoU, which is a major virulence factor of P. aeruginosa. Conclusions. The results of this study indicate an increase in resistance rates to most antibiotics, including carbapenems, and in prevalence of carbapenemase production in nosocomial strains of P. aeruginosa. This can be largely attributed to the expansion of “high-risk” clones.https://cmac-journal.ru/publication/2019/2/cmac-2019-t21-n2-p160/cmac-2019-t21-n2-p160.pdfnosocomial infectionspseudomonas aeruginosaantimicrobial resistancecarbapenemases |