Standard screening methods underreport AAV-mediated transduction and gene editing

Standard screening methods underreport AAV-mediated transduction and gene editing

Conventional methods to detect AAV vector transduction can miss transient or low levels of reporter expression. Here the authors use editing-reporter mice and discover numerous sites of AAV targeting along with better prediction of the gene editing footprint.

Bibliographic Details
Main Authors: Jonathan F. Lang, Sushila A. Toulmin, Kasey L. Brida, Laurence C. Eisenlohr, Beverly L. Davidson
Format: Article
Language:English
Published: Nature Publishing Group 2019-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-11321-7
Description
Summary:Conventional methods to detect AAV vector transduction can miss transient or low levels of reporter expression. Here the authors use editing-reporter mice and discover numerous sites of AAV targeting along with better prediction of the gene editing footprint.
ISSN:2041-1723

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