Standard screening methods underreport AAV-mediated transduction and gene editing

Conventional methods to detect AAV vector transduction can miss transient or low levels of reporter expression. Here the authors use editing-reporter mice and discover numerous sites of AAV targeting along with better prediction of the gene editing footprint.

Bibliographic Details
Main Authors:	Jonathan F. Lang, Sushila A. Toulmin, Kasey L. Brida, Laurence C. Eisenlohr, Beverly L. Davidson
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-11321-7

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spelling	doaj-af6cb7bdc8fb4782a4723ed186a603972021-05-11T12:26:39ZengNature Publishing GroupNature Communications2041-17232019-07-0110111010.1038/s41467-019-11321-7Standard screening methods underreport AAV-mediated transduction and gene editingJonathan F. Lang0Sushila A. Toulmin1Kasey L. Brida2Laurence C. Eisenlohr3Beverly L. Davidson4The Children’s Hospital of PhiladelphiaThe Children’s Hospital of PhiladelphiaThe Children’s Hospital of PhiladelphiaThe Children’s Hospital of PhiladelphiaThe Children’s Hospital of PhiladelphiaConventional methods to detect AAV vector transduction can miss transient or low levels of reporter expression. Here the authors use editing-reporter mice and discover numerous sites of AAV targeting along with better prediction of the gene editing footprint.https://doi.org/10.1038/s41467-019-11321-7
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jonathan F. Lang Sushila A. Toulmin Kasey L. Brida Laurence C. Eisenlohr Beverly L. Davidson
spellingShingle	Jonathan F. Lang Sushila A. Toulmin Kasey L. Brida Laurence C. Eisenlohr Beverly L. Davidson Standard screening methods underreport AAV-mediated transduction and gene editing Nature Communications
author_facet	Jonathan F. Lang Sushila A. Toulmin Kasey L. Brida Laurence C. Eisenlohr Beverly L. Davidson
author_sort	Jonathan F. Lang
title	Standard screening methods underreport AAV-mediated transduction and gene editing
title_short	Standard screening methods underreport AAV-mediated transduction and gene editing
title_full	Standard screening methods underreport AAV-mediated transduction and gene editing
title_fullStr	Standard screening methods underreport AAV-mediated transduction and gene editing
title_full_unstemmed	Standard screening methods underreport AAV-mediated transduction and gene editing
title_sort	standard screening methods underreport aav-mediated transduction and gene editing
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-07-01
description	Conventional methods to detect AAV vector transduction can miss transient or low levels of reporter expression. Here the authors use editing-reporter mice and discover numerous sites of AAV targeting along with better prediction of the gene editing footprint.
url	https://doi.org/10.1038/s41467-019-11321-7
work_keys_str_mv	AT jonathanflang standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting AT sushilaatoulmin standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting AT kaseylbrida standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting AT laurenceceisenlohr standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting AT beverlyldavidson standardscreeningmethodsunderreportaavmediatedtransductionandgeneediting
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Standard screening methods underreport AAV-mediated transduction and gene editing

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