Purificação e caracterização de uma gentioexaose obtida de botriosferana por hidrólise ácida parcial Purification and characterization of a gentiohexaose obtained from botryosphaeran by partial acid hydrolysis

• Main Authors: Iara Ribeiro Silva, Nilson Kobori Monteiro, Paula Felippe Martinez, Nataly Lino Izeli, Ana Flora Dalberto Vasconcelos, Marilsa de Stefani Cardoso, Maria de Lourdes Corradi da Silva, Aneli de Melo Barbosa, Robert F. H. Dekker, Gil Valdo José da Silva, Luís Alberto Beraldo de Moraes
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2008-01-01
• Series: Química Nova
• Subjects: oligosaccharide; partial acid hydrolysis; β-glucan
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000500013

<abstract language="eng">A hexa-oligosaccharide was obtained by partial acid hydrolysis from botryosphaeran, an exopolysaccharide (EPS) &#946;(1&#8594;3; 1&#8594;6)-D-glucan type, produced by the ascomyceteous fungus Botryosphaeria rhodina. The oligosaccharide was purified by gel filtration and charcoal-Celite column chromatography and the analysis was followed by HPAEC/PAD. The structure was determined by NMR spectroscopy and mass spectrometry, which showed that the oligosaccharide consists of six &#946;-D-glucopiranosyl units O-6 substituted (gentiohexaose).