Phytochemical Investigation of New Algerian Lichen Species: <i>Physcia mediterranea</i> Nimis
The present study provides new data concerning the chemical characterisation of <i>Physcia mediterranea </i>Nimis, a rare Mediterranean species belonging to the family Physciaceae. The phytochemical screening was carried out using GC-MS, HPLC-ESI-MS-MS, and NMR techniques. Hot extraction...
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doaj-af854f7b05c746a5a9c5fa3a6cf6701c2021-02-21T00:03:17ZengMDPI AGMolecules1420-30492021-02-01261121112110.3390/molecules26041121Phytochemical Investigation of New Algerian Lichen Species: <i>Physcia mediterranea</i> NimisMarwa Kerboua0Monia Ali Ahmed1Nsevolo Samba2Radhia Aitfella-Lahlou3Lucia Silva4Juan F. Boyero5Cesar Raposo6Jesus Miguel Lopez Rodilla7Laboratory of Vegetal Biology and Environment, Biology Department, Badji Mokhtar University, Annaba 23000, AlgeriaLaboratory of Vegetal Biology and Environment, Biology Department, Badji Mokhtar University, Annaba 23000, AlgeriaChemistry Department, University of Beira Interior, 6201-001 Covilha, PortugalChemistry Department, University of Beira Interior, 6201-001 Covilha, PortugalChemistry Department, University of Beira Interior, 6201-001 Covilha, PortugalDepartment of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemistry, Chromatographic and Mass Analysis Service (NUCLEUS), University of Salamanca, 37008 Salamanca, SpainDepartment of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemistry, Chromatographic and Mass Analysis Service (NUCLEUS), University of Salamanca, 37008 Salamanca, SpainChemistry Department, University of Beira Interior, 6201-001 Covilha, PortugalThe present study provides new data concerning the chemical characterisation of <i>Physcia mediterranea </i>Nimis, a rare Mediterranean species belonging to the family Physciaceae. The phytochemical screening was carried out using GC-MS, HPLC-ESI-MS-MS, and NMR techniques. Hot extraction of n-hexane was carried out, followed by separation of the part insoluble in methanol: wax (WA-hex), from the part soluble in methanol (ME-hex). GC-MS analysis of the ME-hex part revealed the presence of methylbenzoic acids such as sparassol and atraric acid and a diterpene with a kaurene skeleton which has never been detected before in lichen species. Out of all the compounds identified by HPLC-ESI-MS-MS, sixteen compounds are common between WA-hex and ME-hex. Most are aliphatic fatty acids, phenolic compounds and depsides. The wax part is characterised by the presence of atranorin, a depside of high biological value. Proton 1H and carbon 13C NMR have confirmed its identification. Atranol, chloroatranol (depsides compound), Ffukinanolide (sesquiterpene lactones), leprolomin (diphenyl ether), muronic acid (triterpenes), and ursolic acid (triterpenes) have also been identified in ME-hex. The results suggested that <i>Physcia mediterranea</i> Nimis is a valuable source of bioactive compounds that could be useful for several applications as functional foods, cosmetics, and pharmaceuticals.https://www.mdpi.com/1420-3049/26/4/1121Algerian lichen<i>Physcia mediterranea</i> Nimisbioactive compoundsn-hexane extractwaxGC-MS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marwa Kerboua Monia Ali Ahmed Nsevolo Samba Radhia Aitfella-Lahlou Lucia Silva Juan F. Boyero Cesar Raposo Jesus Miguel Lopez Rodilla |
spellingShingle |
Marwa Kerboua Monia Ali Ahmed Nsevolo Samba Radhia Aitfella-Lahlou Lucia Silva Juan F. Boyero Cesar Raposo Jesus Miguel Lopez Rodilla Phytochemical Investigation of New Algerian Lichen Species: <i>Physcia mediterranea</i> Nimis Molecules Algerian lichen <i>Physcia mediterranea</i> Nimis bioactive compounds n-hexane extract wax GC-MS |
author_facet |
Marwa Kerboua Monia Ali Ahmed Nsevolo Samba Radhia Aitfella-Lahlou Lucia Silva Juan F. Boyero Cesar Raposo Jesus Miguel Lopez Rodilla |
author_sort |
Marwa Kerboua |
title |
Phytochemical Investigation of New Algerian Lichen Species: <i>Physcia mediterranea</i> Nimis |
title_short |
Phytochemical Investigation of New Algerian Lichen Species: <i>Physcia mediterranea</i> Nimis |
title_full |
Phytochemical Investigation of New Algerian Lichen Species: <i>Physcia mediterranea</i> Nimis |
title_fullStr |
Phytochemical Investigation of New Algerian Lichen Species: <i>Physcia mediterranea</i> Nimis |
title_full_unstemmed |
Phytochemical Investigation of New Algerian Lichen Species: <i>Physcia mediterranea</i> Nimis |
title_sort |
phytochemical investigation of new algerian lichen species: <i>physcia mediterranea</i> nimis |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-02-01 |
description |
The present study provides new data concerning the chemical characterisation of <i>Physcia mediterranea </i>Nimis, a rare Mediterranean species belonging to the family Physciaceae. The phytochemical screening was carried out using GC-MS, HPLC-ESI-MS-MS, and NMR techniques. Hot extraction of n-hexane was carried out, followed by separation of the part insoluble in methanol: wax (WA-hex), from the part soluble in methanol (ME-hex). GC-MS analysis of the ME-hex part revealed the presence of methylbenzoic acids such as sparassol and atraric acid and a diterpene with a kaurene skeleton which has never been detected before in lichen species. Out of all the compounds identified by HPLC-ESI-MS-MS, sixteen compounds are common between WA-hex and ME-hex. Most are aliphatic fatty acids, phenolic compounds and depsides. The wax part is characterised by the presence of atranorin, a depside of high biological value. Proton 1H and carbon 13C NMR have confirmed its identification. Atranol, chloroatranol (depsides compound), Ffukinanolide (sesquiterpene lactones), leprolomin (diphenyl ether), muronic acid (triterpenes), and ursolic acid (triterpenes) have also been identified in ME-hex. The results suggested that <i>Physcia mediterranea</i> Nimis is a valuable source of bioactive compounds that could be useful for several applications as functional foods, cosmetics, and pharmaceuticals. |
topic |
Algerian lichen <i>Physcia mediterranea</i> Nimis bioactive compounds n-hexane extract wax GC-MS |
url |
https://www.mdpi.com/1420-3049/26/4/1121 |
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