Tannic Acid Improves Renal Function Recovery after Renal Warm Ischemia–Reperfusion in a Rat Model
Background and purpose: Ischemia−reperfusion injury is encountered in numerous processes such as cardiovascular diseases or kidney transplantation; however, the latter involves cold ischemia, different from the warm ischemia found in vascular surgery by arterial clamping. The nature and th...
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doaj-af972329d54148098ab26fadbe7dd8682020-11-25T01:37:45ZengMDPI AGBiomolecules2218-273X2020-03-0110343910.3390/biom10030439biom10030439Tannic Acid Improves Renal Function Recovery after Renal Warm Ischemia–Reperfusion in a Rat ModelLouise Alechinsky0Frederic Favreau1Petra Cechova2Sofiane Inal3Pierre-Antoine Faye4Cecile Ory5Raphaël Thuillier6Benoit Barrou7Patrick Trouillas8Jerome Guillard9Thierry Hauet10INSERM, U1082 IRTOMIT, 86021 Poitiers, FranceUniversité de Limoges, Faculté de Médecine, EA 6309 “Maintenance Myélinique et Neuropathies Périphériques”, 87025 Limoges, FranceUniversity Palacký of Olomouc, RCPTM, Dept Physical Chemistry, Faculty of Science, 771 46 Olomouc, Czech RepublicINSERM, U1082 IRTOMIT, 86021 Poitiers, FranceUniversité de Limoges, Faculté de Médecine, EA 6309 “Maintenance Myélinique et Neuropathies Périphériques”, 87025 Limoges, FranceINSERM, U1082 IRTOMIT, 86021 Poitiers, FranceINSERM, U1082 IRTOMIT, 86021 Poitiers, FranceINSERM, U1082 IRTOMIT, 86021 Poitiers, FranceInserm, UMR 1248, Fac. Pharmacy, Univ. Limoges, 87025 Limoges, FranceUniversité de Poitiers, UMR CNRS 7285 IC2MP, Team 5 Organic Chemistry, 86073 Poitiers, FranceINSERM, U1082 IRTOMIT, 86021 Poitiers, FranceBackground and purpose: Ischemia−reperfusion injury is encountered in numerous processes such as cardiovascular diseases or kidney transplantation; however, the latter involves cold ischemia, different from the warm ischemia found in vascular surgery by arterial clamping. The nature and the intensity of the processes induced by ischemia types are different, hence the therapeutic strategy should be adapted. Herein, we investigated the protective role of tannic acid, a natural polyphenol in a rat model reproducing both renal warm ischemia and kidney allotransplantation. The follow-up was done after 1 week. Experimental approach: To characterize the effect of tannic acid, an in vitro model of endothelial cells subjected to hypoxia−reoxygenation was used. Key results: Tannic acid statistically improved recovery after warm ischemia but not after cold ischemia. In kidneys biopsies, 3 h after warm ischemia−reperfusion, oxidative stress development was limited by tannic acid and the production of reactive oxygen species was inhibited, potentially through Nuclear Factor erythroid-2-Related factor 2 (NRF2) activation. In vitro, tannic acid and its derivatives limited cytotoxicity and the generation of reactive oxygen species. Molecular dynamics simulations showed that tannic acid efficiently interacts with biological membranes, allowing efficient lipid oxidation inhibition. Tannic acid also promoted endothelial cell migration and proliferation during hypoxia. Conclusions: Tannic acid was able to improve renal recovery after renal warm ischemia with an antioxidant effect putatively extended by the production of its derivatives in the body and promoted cell regeneration during hypoxia. This suggests that the mechanisms induced by warm and cold ischemia are different and require specific therapeutic strategies.https://www.mdpi.com/2218-273X/10/3/439warm ischemiacold ischemiatannic acidrenal function recoveryoxidative stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Louise Alechinsky Frederic Favreau Petra Cechova Sofiane Inal Pierre-Antoine Faye Cecile Ory Raphaël Thuillier Benoit Barrou Patrick Trouillas Jerome Guillard Thierry Hauet |
spellingShingle |
Louise Alechinsky Frederic Favreau Petra Cechova Sofiane Inal Pierre-Antoine Faye Cecile Ory Raphaël Thuillier Benoit Barrou Patrick Trouillas Jerome Guillard Thierry Hauet Tannic Acid Improves Renal Function Recovery after Renal Warm Ischemia–Reperfusion in a Rat Model Biomolecules warm ischemia cold ischemia tannic acid renal function recovery oxidative stress |
author_facet |
Louise Alechinsky Frederic Favreau Petra Cechova Sofiane Inal Pierre-Antoine Faye Cecile Ory Raphaël Thuillier Benoit Barrou Patrick Trouillas Jerome Guillard Thierry Hauet |
author_sort |
Louise Alechinsky |
title |
Tannic Acid Improves Renal Function Recovery after Renal Warm Ischemia–Reperfusion in a Rat Model |
title_short |
Tannic Acid Improves Renal Function Recovery after Renal Warm Ischemia–Reperfusion in a Rat Model |
title_full |
Tannic Acid Improves Renal Function Recovery after Renal Warm Ischemia–Reperfusion in a Rat Model |
title_fullStr |
Tannic Acid Improves Renal Function Recovery after Renal Warm Ischemia–Reperfusion in a Rat Model |
title_full_unstemmed |
Tannic Acid Improves Renal Function Recovery after Renal Warm Ischemia–Reperfusion in a Rat Model |
title_sort |
tannic acid improves renal function recovery after renal warm ischemia–reperfusion in a rat model |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2020-03-01 |
description |
Background and purpose: Ischemia−reperfusion injury is encountered in numerous processes such as cardiovascular diseases or kidney transplantation; however, the latter involves cold ischemia, different from the warm ischemia found in vascular surgery by arterial clamping. The nature and the intensity of the processes induced by ischemia types are different, hence the therapeutic strategy should be adapted. Herein, we investigated the protective role of tannic acid, a natural polyphenol in a rat model reproducing both renal warm ischemia and kidney allotransplantation. The follow-up was done after 1 week. Experimental approach: To characterize the effect of tannic acid, an in vitro model of endothelial cells subjected to hypoxia−reoxygenation was used. Key results: Tannic acid statistically improved recovery after warm ischemia but not after cold ischemia. In kidneys biopsies, 3 h after warm ischemia−reperfusion, oxidative stress development was limited by tannic acid and the production of reactive oxygen species was inhibited, potentially through Nuclear Factor erythroid-2-Related factor 2 (NRF2) activation. In vitro, tannic acid and its derivatives limited cytotoxicity and the generation of reactive oxygen species. Molecular dynamics simulations showed that tannic acid efficiently interacts with biological membranes, allowing efficient lipid oxidation inhibition. Tannic acid also promoted endothelial cell migration and proliferation during hypoxia. Conclusions: Tannic acid was able to improve renal recovery after renal warm ischemia with an antioxidant effect putatively extended by the production of its derivatives in the body and promoted cell regeneration during hypoxia. This suggests that the mechanisms induced by warm and cold ischemia are different and require specific therapeutic strategies. |
topic |
warm ischemia cold ischemia tannic acid renal function recovery oxidative stress |
url |
https://www.mdpi.com/2218-273X/10/3/439 |
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