JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production

c-Jun N-terminal kinases (JNKs) regulate cell proliferation and differentiation via phosphorylating such transcription factors as c-Jun. The function of JNKs in retinogenesis remains to be elucidated. Here, we report that knocking out <i>Jnk1</i>, but not <i>Jnk2</i>, increas...

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Main Authors:	Mingyu Pan, Haiyang Hu, Rui Wang, Yi Zhou, Lele Zhang, Chen Wang, Quanyi Wang
Format:	Article
Language:	English
Published:	MDPI AG 2019-08-01
Series:	Cells
Subjects:	JNK1 c-Jun Notch1 opsin photoreceptor retina vision
Online Access:	https://www.mdpi.com/2073-4409/8/9/970

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record_format	Article
spelling	doaj-af9acaaf670d4115b7ab83919bc461d32020-11-25T01:02:57ZengMDPI AGCells2073-44092019-08-018997010.3390/cells8090970cells8090970JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor ProductionMingyu Pan0Haiyang Hu1Rui Wang2Yi Zhou3Lele Zhang4Chen Wang5Quanyi Wang6State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, 639 Longmian Avenue, Jiangning District, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, 639 Longmian Avenue, Jiangning District, Nanjing 211198, ChinaState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, ChinaState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, ChinaState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, ChinaState Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, 639 Longmian Avenue, Jiangning District, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, 639 Longmian Avenue, Jiangning District, Nanjing 211198, Chinac-Jun N-terminal kinases (JNKs) regulate cell proliferation and differentiation via phosphorylating such transcription factors as c-Jun. The function of JNKs in retinogenesis remains to be elucidated. Here, we report that knocking out <i>Jnk1</i>, but not <i>Jnk2</i>, increased the number of photoreceptors, thus enhancing the electroretinogram (ERG) responses. Intriguingly, Notch1, a well-established negative regulator of photoreceptor genesis, was significantly attenuated in <i>Jnk1</i> knockout (KO) mice compared to wild-type mice. Mechanistically, light specifically activated JNK1 to phosphorylate c-Jun, which in turn induced Notch1 transcription. The identified JNK1−c-Jun−Notch1 axis strongly inhibited photoreceptor-related transcriptional factor expression and ultimately impaired photoreceptor opsin expression. Our study uncovered an essential function of JNK1 in retinogenesis, revealing JNK1 as a potential candidate for targeting ophthalmic diseases.https://www.mdpi.com/2073-4409/8/9/970JNK1c-JunNotch1opsinphotoreceptorretinavision
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Mingyu Pan Haiyang Hu Rui Wang Yi Zhou Lele Zhang Chen Wang Quanyi Wang
spellingShingle	Mingyu Pan Haiyang Hu Rui Wang Yi Zhou Lele Zhang Chen Wang Quanyi Wang JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production Cells JNK1 c-Jun Notch1 opsin photoreceptor retina vision
author_facet	Mingyu Pan Haiyang Hu Rui Wang Yi Zhou Lele Zhang Chen Wang Quanyi Wang
author_sort	Mingyu Pan
title	JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production
title_short	JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production
title_full	JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production
title_fullStr	JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production
title_full_unstemmed	JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production
title_sort	jnk1 induces notch1 expression to regulate genes governing photoreceptor production
publisher	MDPI AG
series	Cells
issn	2073-4409
publishDate	2019-08-01
description	c-Jun N-terminal kinases (JNKs) regulate cell proliferation and differentiation via phosphorylating such transcription factors as c-Jun. The function of JNKs in retinogenesis remains to be elucidated. Here, we report that knocking out <i>Jnk1</i>, but not <i>Jnk2</i>, increased the number of photoreceptors, thus enhancing the electroretinogram (ERG) responses. Intriguingly, Notch1, a well-established negative regulator of photoreceptor genesis, was significantly attenuated in <i>Jnk1</i> knockout (KO) mice compared to wild-type mice. Mechanistically, light specifically activated JNK1 to phosphorylate c-Jun, which in turn induced Notch1 transcription. The identified JNK1−c-Jun−Notch1 axis strongly inhibited photoreceptor-related transcriptional factor expression and ultimately impaired photoreceptor opsin expression. Our study uncovered an essential function of JNK1 in retinogenesis, revealing JNK1 as a potential candidate for targeting ophthalmic diseases.
topic	JNK1 c-Jun Notch1 opsin photoreceptor retina vision
url	https://www.mdpi.com/2073-4409/8/9/970
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JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production

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