Opposite Roles of Tumor Cell Proliferation and Immune Cell Infiltration in Postoperative Liver Metastasis of PDAC
BackgroundRecurrence of liver metastasis after pancreatectomy is often a predictor of poor prognosis. Comprehensive genomic analysis may contribute to a better understanding of the molecular mechanisms of postoperative liver metastasis and provide new therapeutic targets.MethodsA total of 67 patient...
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doaj-afa22edf82f049a68deb1905f557b5782021-08-16T06:31:02ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-08-01910.3389/fcell.2021.714718714718Opposite Roles of Tumor Cell Proliferation and Immune Cell Infiltration in Postoperative Liver Metastasis of PDACGuangfu Wang0Guangfu Wang1Shangnan Dai2Shangnan Dai3Hao Gao4Hao Gao5Yong Gao6Yong Gao7Lingdi Yin8Lingdi Yin9Kai Zhang10Kai Zhang11Xumin Huang12Xumin Huang13Zipeng Lu14Zipeng Lu15Yi Miao16Yi Miao17Yi Miao18Pancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaPancreas Institute, Nanjing Medical University, Nanjing, ChinaPancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaPancreas Institute, Nanjing Medical University, Nanjing, ChinaPancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaPancreas Institute, Nanjing Medical University, Nanjing, ChinaPancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaPancreas Institute, Nanjing Medical University, Nanjing, ChinaPancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaPancreas Institute, Nanjing Medical University, Nanjing, ChinaPancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaPancreas Institute, Nanjing Medical University, Nanjing, ChinaPancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaPancreas Institute, Nanjing Medical University, Nanjing, ChinaPancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaPancreas Institute, Nanjing Medical University, Nanjing, ChinaPancreas Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaPancreas Institute, Nanjing Medical University, Nanjing, ChinaPancreas Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, ChinaBackgroundRecurrence of liver metastasis after pancreatectomy is often a predictor of poor prognosis. Comprehensive genomic analysis may contribute to a better understanding of the molecular mechanisms of postoperative liver metastasis and provide new therapeutic targets.MethodsA total of 67 patients from The Cancer Genome Atlas (TCGA) were included in this study. We analyzed differentially expressed genes (DEGs) by R package “DESeq2.” Weighted gene co-expression network analysis (WGCNA) was applied to investigate the key modules and hub genes. Immunohistochemistry was used to analyze tumor cell proliferation index and CD4+ T cells infiltration.ResultsFunctional analysis of DEGs between the liver metastatic and recurrence-free groups was mainly concentrated in the immune response. The liver metastasis group had lower immune and stroma scores and a higher TP53 mutation rate. WGCNA showed that the genes in key modules related to disease-free survival (DFS) and overall survival (OS) were mainly enriched in the cell proliferation process and tumor immune response. Immunohistochemical analysis showed that the pancreatic cancer cells of patients with early postoperative liver metastasis had higher proliferative activity, while the infiltration of CD4+ T cells in tumor specimens was less.ConclusionOur study suggested that increased immune cell infiltration (especially CD4+ T cells) and tumor cell proliferation may play an opposite role in liver metastasis recurrence after pancreatic cancer.https://www.frontiersin.org/articles/10.3389/fcell.2021.714718/fullpancreatic cancerliver metastasisweighted gene co-expression network analysesimmune cell infiltrationcell proliferation |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guangfu Wang Guangfu Wang Shangnan Dai Shangnan Dai Hao Gao Hao Gao Yong Gao Yong Gao Lingdi Yin Lingdi Yin Kai Zhang Kai Zhang Xumin Huang Xumin Huang Zipeng Lu Zipeng Lu Yi Miao Yi Miao Yi Miao |
spellingShingle |
Guangfu Wang Guangfu Wang Shangnan Dai Shangnan Dai Hao Gao Hao Gao Yong Gao Yong Gao Lingdi Yin Lingdi Yin Kai Zhang Kai Zhang Xumin Huang Xumin Huang Zipeng Lu Zipeng Lu Yi Miao Yi Miao Yi Miao Opposite Roles of Tumor Cell Proliferation and Immune Cell Infiltration in Postoperative Liver Metastasis of PDAC Frontiers in Cell and Developmental Biology pancreatic cancer liver metastasis weighted gene co-expression network analyses immune cell infiltration cell proliferation |
author_facet |
Guangfu Wang Guangfu Wang Shangnan Dai Shangnan Dai Hao Gao Hao Gao Yong Gao Yong Gao Lingdi Yin Lingdi Yin Kai Zhang Kai Zhang Xumin Huang Xumin Huang Zipeng Lu Zipeng Lu Yi Miao Yi Miao Yi Miao |
author_sort |
Guangfu Wang |
title |
Opposite Roles of Tumor Cell Proliferation and Immune Cell Infiltration in Postoperative Liver Metastasis of PDAC |
title_short |
Opposite Roles of Tumor Cell Proliferation and Immune Cell Infiltration in Postoperative Liver Metastasis of PDAC |
title_full |
Opposite Roles of Tumor Cell Proliferation and Immune Cell Infiltration in Postoperative Liver Metastasis of PDAC |
title_fullStr |
Opposite Roles of Tumor Cell Proliferation and Immune Cell Infiltration in Postoperative Liver Metastasis of PDAC |
title_full_unstemmed |
Opposite Roles of Tumor Cell Proliferation and Immune Cell Infiltration in Postoperative Liver Metastasis of PDAC |
title_sort |
opposite roles of tumor cell proliferation and immune cell infiltration in postoperative liver metastasis of pdac |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2021-08-01 |
description |
BackgroundRecurrence of liver metastasis after pancreatectomy is often a predictor of poor prognosis. Comprehensive genomic analysis may contribute to a better understanding of the molecular mechanisms of postoperative liver metastasis and provide new therapeutic targets.MethodsA total of 67 patients from The Cancer Genome Atlas (TCGA) were included in this study. We analyzed differentially expressed genes (DEGs) by R package “DESeq2.” Weighted gene co-expression network analysis (WGCNA) was applied to investigate the key modules and hub genes. Immunohistochemistry was used to analyze tumor cell proliferation index and CD4+ T cells infiltration.ResultsFunctional analysis of DEGs between the liver metastatic and recurrence-free groups was mainly concentrated in the immune response. The liver metastasis group had lower immune and stroma scores and a higher TP53 mutation rate. WGCNA showed that the genes in key modules related to disease-free survival (DFS) and overall survival (OS) were mainly enriched in the cell proliferation process and tumor immune response. Immunohistochemical analysis showed that the pancreatic cancer cells of patients with early postoperative liver metastasis had higher proliferative activity, while the infiltration of CD4+ T cells in tumor specimens was less.ConclusionOur study suggested that increased immune cell infiltration (especially CD4+ T cells) and tumor cell proliferation may play an opposite role in liver metastasis recurrence after pancreatic cancer. |
topic |
pancreatic cancer liver metastasis weighted gene co-expression network analyses immune cell infiltration cell proliferation |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2021.714718/full |
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