Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation
Despite its advantages for pig breeding, embryo transfer (ET) has a major handicap: high embryo mortality during the pre- and implantation period, probably caused by divergent phenomena of tolerance between the immunologically unrelated (i.e., allogeneic) embryos and the recipient sow. Thus, to reac...
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Format: | Article |
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Frontiers Media S.A.
2020-11-01
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Series: | Frontiers in Veterinary Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2020.611598/full |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Josep M. Cambra Josep M. Cambra Josep M. Cambra Amaia Jauregi-Miguel Amaia Jauregi-Miguel Manuel Alvarez-Rodriguez Inmaculada Parrilla Inmaculada Parrilla Maria A. Gil Maria A. Gil Emilio A. Martinez Emilio A. Martinez Cristina Cuello Cristina Cuello Heriberto Rodriguez-Martinez Cristina A. Martinez |
spellingShingle |
Josep M. Cambra Josep M. Cambra Josep M. Cambra Amaia Jauregi-Miguel Amaia Jauregi-Miguel Manuel Alvarez-Rodriguez Inmaculada Parrilla Inmaculada Parrilla Maria A. Gil Maria A. Gil Emilio A. Martinez Emilio A. Martinez Cristina Cuello Cristina Cuello Heriberto Rodriguez-Martinez Cristina A. Martinez Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation Frontiers in Veterinary Science LIF LIF receptor embryo transfer endometrium immunotolerance allogeneic pregnancy |
author_facet |
Josep M. Cambra Josep M. Cambra Josep M. Cambra Amaia Jauregi-Miguel Amaia Jauregi-Miguel Manuel Alvarez-Rodriguez Inmaculada Parrilla Inmaculada Parrilla Maria A. Gil Maria A. Gil Emilio A. Martinez Emilio A. Martinez Cristina Cuello Cristina Cuello Heriberto Rodriguez-Martinez Cristina A. Martinez |
author_sort |
Josep M. Cambra |
title |
Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation |
title_short |
Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation |
title_full |
Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation |
title_fullStr |
Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation |
title_full_unstemmed |
Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation |
title_sort |
allogeneic embryos disregulate leukemia inhibitory factor (lif) and its receptor in the porcine endometrium during implantation |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2020-11-01 |
description |
Despite its advantages for pig breeding, embryo transfer (ET) has a major handicap: high embryo mortality during the pre- and implantation period, probably caused by divergent phenomena of tolerance between the immunologically unrelated (i.e., allogeneic) embryos and the recipient sow. Thus, to reach a similar maternal tolerance as in conventional breeding by artificial insemination (AI) would be the key to ET-success. For this reason, we studied the expression of the leukemia inhibitory factor (LIF) cytokine and its receptor in the pig endometrium during the implantation period (days 18 and 24) in sows subjected to ET (AL group) vs. post-cervical-AI controls (Hemi-AL group). Quantification of expression was performed at both mRNA (rt-qPCR) and protein (WB) levels. The expression of endometrial LIF on day 24 was considerably lower in ET than in AI pregnancies. Correlations between endometrial mRNA levels of LIF and LIF-R showed that, contrary to early AI-pregnancies, ET-pregnancies lack an inverse relation between cytokine and receptor levels. In conclusion, ET-pregnancies lack sufficient endometrial levels of LIF to develop adequate immunotolerance mechanisms to prevent the rejection of allogeneic ET-embryos. |
topic |
LIF LIF receptor embryo transfer endometrium immunotolerance allogeneic pregnancy |
url |
https://www.frontiersin.org/articles/10.3389/fvets.2020.611598/full |
work_keys_str_mv |
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doaj-afaf88c49f7445d6b8528ae7e1d2c4c02020-11-25T04:12:20ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-11-01710.3389/fvets.2020.611598611598Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During ImplantationJosep M. Cambra0Josep M. Cambra1Josep M. Cambra2Amaia Jauregi-Miguel3Amaia Jauregi-Miguel4Manuel Alvarez-Rodriguez5Inmaculada Parrilla6Inmaculada Parrilla7Maria A. Gil8Maria A. Gil9Emilio A. Martinez10Emilio A. Martinez11Cristina Cuello12Cristina Cuello13Heriberto Rodriguez-Martinez14Cristina A. Martinez15Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia, SpainInstitute for Biomedical Research of Murcia (IMIB-Arrixaca), Campus de Ciencias de la Salud, Murcia, SpainDepartment of Biomedical & Clinical Sciences (BKV), BKH/Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Linköping University, Linköping, SwedenDepartment of Biomedical & Clinical Sciences (BKV), BKH/Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Linköping University, Linköping, SwedenWallenberg Centre for Molecular Medicine, Linköping University, Linköping, SwedenDepartment of Biomedical & Clinical Sciences (BKV), BKH/Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Linköping University, Linköping, SwedenDepartment of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia, SpainInstitute for Biomedical Research of Murcia (IMIB-Arrixaca), Campus de Ciencias de la Salud, Murcia, SpainDepartment of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia, SpainInstitute for Biomedical Research of Murcia (IMIB-Arrixaca), Campus de Ciencias de la Salud, Murcia, SpainDepartment of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia, SpainInstitute for Biomedical Research of Murcia (IMIB-Arrixaca), Campus de Ciencias de la Salud, Murcia, SpainDepartment of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia, SpainInstitute for Biomedical Research of Murcia (IMIB-Arrixaca), Campus de Ciencias de la Salud, Murcia, SpainDepartment of Biomedical & Clinical Sciences (BKV), BKH/Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Linköping University, Linköping, SwedenDepartment of Biomedical & Clinical Sciences (BKV), BKH/Obstetrics & Gynaecology, Faculty of Medicine and Health Sciences, Linköping University, Linköping, SwedenDespite its advantages for pig breeding, embryo transfer (ET) has a major handicap: high embryo mortality during the pre- and implantation period, probably caused by divergent phenomena of tolerance between the immunologically unrelated (i.e., allogeneic) embryos and the recipient sow. Thus, to reach a similar maternal tolerance as in conventional breeding by artificial insemination (AI) would be the key to ET-success. For this reason, we studied the expression of the leukemia inhibitory factor (LIF) cytokine and its receptor in the pig endometrium during the implantation period (days 18 and 24) in sows subjected to ET (AL group) vs. post-cervical-AI controls (Hemi-AL group). Quantification of expression was performed at both mRNA (rt-qPCR) and protein (WB) levels. The expression of endometrial LIF on day 24 was considerably lower in ET than in AI pregnancies. Correlations between endometrial mRNA levels of LIF and LIF-R showed that, contrary to early AI-pregnancies, ET-pregnancies lack an inverse relation between cytokine and receptor levels. In conclusion, ET-pregnancies lack sufficient endometrial levels of LIF to develop adequate immunotolerance mechanisms to prevent the rejection of allogeneic ET-embryos.https://www.frontiersin.org/articles/10.3389/fvets.2020.611598/fullLIFLIF receptorembryo transferendometriumimmunotoleranceallogeneic pregnancy |