Exosomal TUBB3 mRNA expression of metastatic castration‐resistant prostate cancer patients: Association with patient outcome under abiraterone
Abstract Background To use ddPCR to quantify plasma exosomal class III β‐tubulin (βIII‐tubulin, TUBB3, encoded by the TUBB3 gene) mRNA expression in metastatic castration‐resistant prostate cancer (mCRPC) patients, and study the association of this expression with abiraterone efficacy. Methods Blood...
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Online Access: | https://doi.org/10.1002/cam4.4168 |
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doaj-afba6bcc8cfe48b4bec7cec175fac6b42021-09-17T05:24:00ZengWileyCancer Medicine2045-76342021-09-0110186282629010.1002/cam4.4168Exosomal TUBB3 mRNA expression of metastatic castration‐resistant prostate cancer patients: Association with patient outcome under abirateroneSha Zhu0Yuchao Ni1Guangxi Sun2Zilin Wang3Junru Chen4Xingming Zhang5Jinge Zhao6Xudong Zhu7Jindong Dai8Zhenhua Liu9Jiayu Liang10Haoran Zhang11Yaowen Zhang12Pengfei Shen13Hao Zeng14Department of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaDepartment of Urology Institute of Urology West China HospitalSichuan University Chengdu Sichuan ChinaAbstract Background To use ddPCR to quantify plasma exosomal class III β‐tubulin (βIII‐tubulin, TUBB3, encoded by the TUBB3 gene) mRNA expression in metastatic castration‐resistant prostate cancer (mCRPC) patients, and study the association of this expression with abiraterone efficacy. Methods Blood samples were prospectively collected from 52 mCRPC patients using abiraterone as first‐line therapy to measure plasma exosomal TUBB3 mRNA expression value before the initiation of abiraterone. Study endpoints were PSA response rate, PSA‐progression‐free survival (PSA‐PFS), and overall survival (OS, from CRPC to death). Results Patients with positive exosomal TUBB3 expression showed shorter PSA‐PFS (negative TUBB3 vs. positive TUBB3: 11.0 vs. 7.9 months; p = 0.014). Further analysis demonstrated that patients with strongly positive exosomal TUBB3 (>20 copies/20 µl) was associated with even shorter PSA‐PFS (negative TUBB3 vs. positive TUBB3 [<20 copies/20 µl] vs. strongly positive TUBB3 [>20 copies/20 µl]: 11.0 vs. 8.3 vs. 3.6 months, p = 0.005). In multivariate analyzes, TUBB3 (+) (HR: 2.114, p = 0.033) and ECOG score >2 (HR: 3.039, p = 0.006) were independent prognosticators of poor PSA‐PFS. PSA response and OS did not present significant differences. Conclusion The exosomal TUBB3 mRNA expression level is associated with poor PSA‐PFS of abiraterone in mCRPC patients. The detection of exosomal TUBB3 can be valuable in their management.https://doi.org/10.1002/cam4.4168abiraterone acetateexosomesmessengerprostatic neoplasmsRNATUBB3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sha Zhu Yuchao Ni Guangxi Sun Zilin Wang Junru Chen Xingming Zhang Jinge Zhao Xudong Zhu Jindong Dai Zhenhua Liu Jiayu Liang Haoran Zhang Yaowen Zhang Pengfei Shen Hao Zeng |
spellingShingle |
Sha Zhu Yuchao Ni Guangxi Sun Zilin Wang Junru Chen Xingming Zhang Jinge Zhao Xudong Zhu Jindong Dai Zhenhua Liu Jiayu Liang Haoran Zhang Yaowen Zhang Pengfei Shen Hao Zeng Exosomal TUBB3 mRNA expression of metastatic castration‐resistant prostate cancer patients: Association with patient outcome under abiraterone Cancer Medicine abiraterone acetate exosomes messenger prostatic neoplasms RNA TUBB3 |
author_facet |
Sha Zhu Yuchao Ni Guangxi Sun Zilin Wang Junru Chen Xingming Zhang Jinge Zhao Xudong Zhu Jindong Dai Zhenhua Liu Jiayu Liang Haoran Zhang Yaowen Zhang Pengfei Shen Hao Zeng |
author_sort |
Sha Zhu |
title |
Exosomal TUBB3 mRNA expression of metastatic castration‐resistant prostate cancer patients: Association with patient outcome under abiraterone |
title_short |
Exosomal TUBB3 mRNA expression of metastatic castration‐resistant prostate cancer patients: Association with patient outcome under abiraterone |
title_full |
Exosomal TUBB3 mRNA expression of metastatic castration‐resistant prostate cancer patients: Association with patient outcome under abiraterone |
title_fullStr |
Exosomal TUBB3 mRNA expression of metastatic castration‐resistant prostate cancer patients: Association with patient outcome under abiraterone |
title_full_unstemmed |
Exosomal TUBB3 mRNA expression of metastatic castration‐resistant prostate cancer patients: Association with patient outcome under abiraterone |
title_sort |
exosomal tubb3 mrna expression of metastatic castration‐resistant prostate cancer patients: association with patient outcome under abiraterone |
publisher |
Wiley |
series |
Cancer Medicine |
issn |
2045-7634 |
publishDate |
2021-09-01 |
description |
Abstract Background To use ddPCR to quantify plasma exosomal class III β‐tubulin (βIII‐tubulin, TUBB3, encoded by the TUBB3 gene) mRNA expression in metastatic castration‐resistant prostate cancer (mCRPC) patients, and study the association of this expression with abiraterone efficacy. Methods Blood samples were prospectively collected from 52 mCRPC patients using abiraterone as first‐line therapy to measure plasma exosomal TUBB3 mRNA expression value before the initiation of abiraterone. Study endpoints were PSA response rate, PSA‐progression‐free survival (PSA‐PFS), and overall survival (OS, from CRPC to death). Results Patients with positive exosomal TUBB3 expression showed shorter PSA‐PFS (negative TUBB3 vs. positive TUBB3: 11.0 vs. 7.9 months; p = 0.014). Further analysis demonstrated that patients with strongly positive exosomal TUBB3 (>20 copies/20 µl) was associated with even shorter PSA‐PFS (negative TUBB3 vs. positive TUBB3 [<20 copies/20 µl] vs. strongly positive TUBB3 [>20 copies/20 µl]: 11.0 vs. 8.3 vs. 3.6 months, p = 0.005). In multivariate analyzes, TUBB3 (+) (HR: 2.114, p = 0.033) and ECOG score >2 (HR: 3.039, p = 0.006) were independent prognosticators of poor PSA‐PFS. PSA response and OS did not present significant differences. Conclusion The exosomal TUBB3 mRNA expression level is associated with poor PSA‐PFS of abiraterone in mCRPC patients. The detection of exosomal TUBB3 can be valuable in their management. |
topic |
abiraterone acetate exosomes messenger prostatic neoplasms RNA TUBB3 |
url |
https://doi.org/10.1002/cam4.4168 |
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