A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals

A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals

Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensab...

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Main Authors: Miki Okumura, Akiko Takenaka-Uema, Shin Murakami, Taisuke Horimoto
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:Veterinary Sciences
Subjects:
influenza virus
hemagglutinin
subtype
enzyme-linked immunosorbent assay
boar
raccoon
Online Access:https://www.mdpi.com/2306-7381/6/3/64
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spelling doaj-afc04a71aaf24809b15dbe828885d2622021-04-02T09:04:42ZengMDPI AGVeterinary Sciences2306-73812019-07-01636410.3390/vetsci6030064vetsci6030064A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in AnimalsMiki Okumura0Akiko Takenaka-Uema1Shin Murakami2Taisuke Horimoto3Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanMammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of an enzyme-linked immunosorbent assay as a rapid diagnosis method, using a panel of HA subtype antigens. By analyzing reference immune sera, we found that this novel assay could detect HA subtype-specific antibodies without considerable inter-subtypic cross-reactivities, contributing to diagnosis of influenza virus infection.https://www.mdpi.com/2306-7381/6/3/64influenza virushemagglutininsubtypeenzyme-linked immunosorbent assayboarraccoon
collection DOAJ
language English
format Article
sources DOAJ
author Miki Okumura
Akiko Takenaka-Uema
Shin Murakami
Taisuke Horimoto
spellingShingle Miki Okumura
Akiko Takenaka-Uema
Shin Murakami
Taisuke Horimoto
A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
Veterinary Sciences
influenza virus
hemagglutinin
subtype
enzyme-linked immunosorbent assay
boar
raccoon
author_facet Miki Okumura
Akiko Takenaka-Uema
Shin Murakami
Taisuke Horimoto
author_sort Miki Okumura
title A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_short A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_full A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_fullStr A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_full_unstemmed A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
title_sort multi-hemagglutinin-based enzyme-linked immunosorbent assay to serologically detect influenza a virus infection in animals
publisher MDPI AG
series Veterinary Sciences
issn 2306-7381
publishDate 2019-07-01
description Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of an enzyme-linked immunosorbent assay as a rapid diagnosis method, using a panel of HA subtype antigens. By analyzing reference immune sera, we found that this novel assay could detect HA subtype-specific antibodies without considerable inter-subtypic cross-reactivities, contributing to diagnosis of influenza virus infection.
topic influenza virus
hemagglutinin
subtype
enzyme-linked immunosorbent assay
boar
raccoon
url https://www.mdpi.com/2306-7381/6/3/64
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AT akikotakenakauema amultihemagglutininbasedenzymelinkedimmunosorbentassaytoserologicallydetectinfluenzaavirusinfectioninanimals
AT shinmurakami amultihemagglutininbasedenzymelinkedimmunosorbentassaytoserologicallydetectinfluenzaavirusinfectioninanimals
AT taisukehorimoto amultihemagglutininbasedenzymelinkedimmunosorbentassaytoserologicallydetectinfluenzaavirusinfectioninanimals
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