A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals
Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensab...
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doaj-afc04a71aaf24809b15dbe828885d2622021-04-02T09:04:42ZengMDPI AGVeterinary Sciences2306-73812019-07-01636410.3390/vetsci6030064vetsci6030064A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in AnimalsMiki Okumura0Akiko Takenaka-Uema1Shin Murakami2Taisuke Horimoto3Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanMammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of an enzyme-linked immunosorbent assay as a rapid diagnosis method, using a panel of HA subtype antigens. By analyzing reference immune sera, we found that this novel assay could detect HA subtype-specific antibodies without considerable inter-subtypic cross-reactivities, contributing to diagnosis of influenza virus infection.https://www.mdpi.com/2306-7381/6/3/64influenza virushemagglutininsubtypeenzyme-linked immunosorbent assayboarraccoon |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miki Okumura Akiko Takenaka-Uema Shin Murakami Taisuke Horimoto |
spellingShingle |
Miki Okumura Akiko Takenaka-Uema Shin Murakami Taisuke Horimoto A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals Veterinary Sciences influenza virus hemagglutinin subtype enzyme-linked immunosorbent assay boar raccoon |
author_facet |
Miki Okumura Akiko Takenaka-Uema Shin Murakami Taisuke Horimoto |
author_sort |
Miki Okumura |
title |
A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals |
title_short |
A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals |
title_full |
A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals |
title_fullStr |
A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals |
title_full_unstemmed |
A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals |
title_sort |
multi-hemagglutinin-based enzyme-linked immunosorbent assay to serologically detect influenza a virus infection in animals |
publisher |
MDPI AG |
series |
Veterinary Sciences |
issn |
2306-7381 |
publishDate |
2019-07-01 |
description |
Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of an enzyme-linked immunosorbent assay as a rapid diagnosis method, using a panel of HA subtype antigens. By analyzing reference immune sera, we found that this novel assay could detect HA subtype-specific antibodies without considerable inter-subtypic cross-reactivities, contributing to diagnosis of influenza virus infection. |
topic |
influenza virus hemagglutinin subtype enzyme-linked immunosorbent assay boar raccoon |
url |
https://www.mdpi.com/2306-7381/6/3/64 |
work_keys_str_mv |
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