Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual

Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual

Reprogramming of somatic cells with higher genome integrity, and use of non-integrating gene delivery methods and xeno-free cell culture conditions aid in the generation of iPSCs which are more suitable for disease modelling and clinical applications. We describe here an iPSC line generated using su...

Full description

Bibliographic Details
Main Authors: Kannan V. Manian, Sumitha P. Bharathan, Madhavi Maddali, Vivi M. Srivastava, Alok Srivastava, Shaji Ramachandran Velayudhan
Format: Article
Language:English
Published: Elsevier 2018-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506118300989
id doaj-afc7bb6678684a2bae7f0d5e7896a807
record_format Article
spelling doaj-afc7bb6678684a2bae7f0d5e7896a8072020-11-24T21:00:45ZengElsevierStem Cell Research1873-50612018-05-0129148151Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individualKannan V. Manian0Sumitha P. Bharathan1Madhavi Maddali2Vivi M. Srivastava3Alok Srivastava4Shaji Ramachandran Velayudhan5Centre for Stem Cell Research, Christian Medical College, Vellore, IndiaCentre for Stem Cell Research, Christian Medical College, Vellore, IndiaDepartment of Haematology, Christian Medical College, Vellore, IndiaDepartment of Clinical Cytogenetics, Christian Medical College, Vellore, IndiaCentre for Stem Cell Research, Christian Medical College, Vellore, India; Department of Haematology, Christian Medical College, Vellore, IndiaCentre for Stem Cell Research, Christian Medical College, Vellore, India; Department of Haematology, Christian Medical College, Vellore, India; Corresponding author at: Department of Haematology and Centre for Stem Cell Research, Christian Medical College, Vellore, India.Reprogramming of somatic cells with higher genome integrity, and use of non-integrating gene delivery methods and xeno-free cell culture conditions aid in the generation of iPSCs which are more suitable for disease modelling and clinical applications. We describe here an iPSC line generated using such conditions, which expressed all the pluripotency markers, retained normal karyotype and exhibited the potential for tri-lineage differentiation, both in-vitro and in-vivo. This is the first iPSC line available from a healthy Indian individual for researchers.http://www.sciencedirect.com/science/article/pii/S1873506118300989
collection DOAJ
language English
format Article
sources DOAJ
author Kannan V. Manian
Sumitha P. Bharathan
Madhavi Maddali
Vivi M. Srivastava
Alok Srivastava
Shaji Ramachandran Velayudhan
spellingShingle Kannan V. Manian
Sumitha P. Bharathan
Madhavi Maddali
Vivi M. Srivastava
Alok Srivastava
Shaji Ramachandran Velayudhan
Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual
Stem Cell Research
author_facet Kannan V. Manian
Sumitha P. Bharathan
Madhavi Maddali
Vivi M. Srivastava
Alok Srivastava
Shaji Ramachandran Velayudhan
author_sort Kannan V. Manian
title Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual
title_short Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual
title_full Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual
title_fullStr Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual
title_full_unstemmed Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual
title_sort generation of an integration-free ipsc line (cscri005-a) from erythroid progenitor cells of a healthy indian male individual
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2018-05-01
description Reprogramming of somatic cells with higher genome integrity, and use of non-integrating gene delivery methods and xeno-free cell culture conditions aid in the generation of iPSCs which are more suitable for disease modelling and clinical applications. We describe here an iPSC line generated using such conditions, which expressed all the pluripotency markers, retained normal karyotype and exhibited the potential for tri-lineage differentiation, both in-vitro and in-vivo. This is the first iPSC line available from a healthy Indian individual for researchers.
url http://www.sciencedirect.com/science/article/pii/S1873506118300989
work_keys_str_mv AT kannanvmanian generationofanintegrationfreeipsclinecscri005afromerythroidprogenitorcellsofahealthyindianmaleindividual
AT sumithapbharathan generationofanintegrationfreeipsclinecscri005afromerythroidprogenitorcellsofahealthyindianmaleindividual
AT madhavimaddali generationofanintegrationfreeipsclinecscri005afromerythroidprogenitorcellsofahealthyindianmaleindividual
AT vivimsrivastava generationofanintegrationfreeipsclinecscri005afromerythroidprogenitorcellsofahealthyindianmaleindividual
AT aloksrivastava generationofanintegrationfreeipsclinecscri005afromerythroidprogenitorcellsofahealthyindianmaleindividual
AT shajiramachandranvelayudhan generationofanintegrationfreeipsclinecscri005afromerythroidprogenitorcellsofahealthyindianmaleindividual
_version_ 1716778944498237440

Similar Items