A rapid chemical method for quantification of lipids separated by thin-layer chromatography

Lipids, separated by thin-layer chromatography, are oxidized in an acid dichromate solution. The reduction in absorbance at 350 mμ is proportional to the amount of lipid. One reagent suffices to quantify all lipid classes to a lower limit of 15 μg of lipid.

Bibliographic Details
Main Author: J.S. Amenta
Format: Article
Language:English
Published: Elsevier 1964-04-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520402512
Description
Summary:Lipids, separated by thin-layer chromatography, are oxidized in an acid dichromate solution. The reduction in absorbance at 350 mμ is proportional to the amount of lipid. One reagent suffices to quantify all lipid classes to a lower limit of 15 μg of lipid.
ISSN:0022-2275