The Synechocystis sp. PCC 6803 Genome Encodes Up to Four 2-Phosphoglycolate Phosphatases
Photorespiratory phosphoglycolate (2PG) metabolism is essential for cyanobacteria, algae, and plants. The first enzyme of the pathway, 2PG phosphatase (PGPase), is known from plants and algae but was scarcely investigated in cyanobacteria. In silico analysis revealed four candidate genes (slr0458, s...
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doaj-afe3e0fb3d92445ea2e95c45779a91c52020-11-24T21:45:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-11-01910.3389/fpls.2018.01718426806The Synechocystis sp. PCC 6803 Genome Encodes Up to Four 2-Phosphoglycolate PhosphatasesSnigdha Rai0Snigdha Rai1Stefan Lucius2Ramona Kern3Hermann Bauwe4Aaron Kaplan5Joachim Kopka6Martin Hagemann7Martin Hagemann8Department of Plant Physiology, University of Rostock, Rostock, GermanyCentre of Advanced Study in Botany, Banaras Hindu University, Varanasi, IndiaDepartment of Plant Physiology, University of Rostock, Rostock, GermanyDepartment of Plant Physiology, University of Rostock, Rostock, GermanyDepartment of Plant Physiology, University of Rostock, Rostock, GermanyDepartment of Plant and Environmental Sciences, The Hebrew University of Jerusalem, Jerusalem, IsraelApplied Metabolome Analysis, Department of Molecular Physiology, Max Planck Institute of Molecular Plant Physiology, Potsdam, GermanyDepartment of Plant Physiology, University of Rostock, Rostock, GermanyDepartment Life, Light and Matter, University of Rostock, Rostock, GermanyPhotorespiratory phosphoglycolate (2PG) metabolism is essential for cyanobacteria, algae, and plants. The first enzyme of the pathway, 2PG phosphatase (PGPase), is known from plants and algae but was scarcely investigated in cyanobacteria. In silico analysis revealed four candidate genes (slr0458, slr0586, sll1349, and slr1762) in the genome of the model cyanobacterium Synechocystis sp. PCC 6803 that all belong to the 2-haloacid dehalogenase (HAD) superfamily and could possibly encode PGPase proteins. However, in contrast to known algal and plant PGPases, the putative cyanobacterial PGPases belong to another HAD subfamily implying that PGPases in eukaryotic phototrophs did not originate from cyanobacterial PGPases. To verify their function, these four genes were inactivated both individually and in combination. A mild high-CO2-requiring (HCR) growth phenotype typical for photorespiratory mutants was observed only in Δsll1349. Combinatorial inactivation enhanced the HCR phenotype in specific double and triple mutants. Heterologous expression of the putative cyanobacterial PGPases in E. coli led to higher PGPase activities in crude cell extracts, but only the purified Slr0458 protein showed PGPase activity. Hence, we propose that a consortium of up to four photorespiratory PGPases may initiate photorespiratory 2PG metabolism in Synechocystis. We suggest that redundancy of this essential enzyme activity could be related to the highly adaptive lifestyle of cyanobacteria such as Synechocystis sp. PCC 6803, which allows them to grow under very diverse conditions.https://www.frontiersin.org/article/10.3389/fpls.2018.01718/fullcyanobacteria2-haloacid dehalogenasemutantphosphoglycolate phosphatasephotorespirationSynechocystis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Snigdha Rai Snigdha Rai Stefan Lucius Ramona Kern Hermann Bauwe Aaron Kaplan Joachim Kopka Martin Hagemann Martin Hagemann |
spellingShingle |
Snigdha Rai Snigdha Rai Stefan Lucius Ramona Kern Hermann Bauwe Aaron Kaplan Joachim Kopka Martin Hagemann Martin Hagemann The Synechocystis sp. PCC 6803 Genome Encodes Up to Four 2-Phosphoglycolate Phosphatases Frontiers in Plant Science cyanobacteria 2-haloacid dehalogenase mutant phosphoglycolate phosphatase photorespiration Synechocystis |
author_facet |
Snigdha Rai Snigdha Rai Stefan Lucius Ramona Kern Hermann Bauwe Aaron Kaplan Joachim Kopka Martin Hagemann Martin Hagemann |
author_sort |
Snigdha Rai |
title |
The Synechocystis sp. PCC 6803 Genome Encodes Up to Four 2-Phosphoglycolate Phosphatases |
title_short |
The Synechocystis sp. PCC 6803 Genome Encodes Up to Four 2-Phosphoglycolate Phosphatases |
title_full |
The Synechocystis sp. PCC 6803 Genome Encodes Up to Four 2-Phosphoglycolate Phosphatases |
title_fullStr |
The Synechocystis sp. PCC 6803 Genome Encodes Up to Four 2-Phosphoglycolate Phosphatases |
title_full_unstemmed |
The Synechocystis sp. PCC 6803 Genome Encodes Up to Four 2-Phosphoglycolate Phosphatases |
title_sort |
synechocystis sp. pcc 6803 genome encodes up to four 2-phosphoglycolate phosphatases |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2018-11-01 |
description |
Photorespiratory phosphoglycolate (2PG) metabolism is essential for cyanobacteria, algae, and plants. The first enzyme of the pathway, 2PG phosphatase (PGPase), is known from plants and algae but was scarcely investigated in cyanobacteria. In silico analysis revealed four candidate genes (slr0458, slr0586, sll1349, and slr1762) in the genome of the model cyanobacterium Synechocystis sp. PCC 6803 that all belong to the 2-haloacid dehalogenase (HAD) superfamily and could possibly encode PGPase proteins. However, in contrast to known algal and plant PGPases, the putative cyanobacterial PGPases belong to another HAD subfamily implying that PGPases in eukaryotic phototrophs did not originate from cyanobacterial PGPases. To verify their function, these four genes were inactivated both individually and in combination. A mild high-CO2-requiring (HCR) growth phenotype typical for photorespiratory mutants was observed only in Δsll1349. Combinatorial inactivation enhanced the HCR phenotype in specific double and triple mutants. Heterologous expression of the putative cyanobacterial PGPases in E. coli led to higher PGPase activities in crude cell extracts, but only the purified Slr0458 protein showed PGPase activity. Hence, we propose that a consortium of up to four photorespiratory PGPases may initiate photorespiratory 2PG metabolism in Synechocystis. We suggest that redundancy of this essential enzyme activity could be related to the highly adaptive lifestyle of cyanobacteria such as Synechocystis sp. PCC 6803, which allows them to grow under very diverse conditions. |
topic |
cyanobacteria 2-haloacid dehalogenase mutant phosphoglycolate phosphatase photorespiration Synechocystis |
url |
https://www.frontiersin.org/article/10.3389/fpls.2018.01718/full |
work_keys_str_mv |
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