First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)

We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikt...

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Main Authors:	M R Trapani, M G Parisi, M Toubiana, L Coquet, T Jouenne, P Roch, M Cammarata
Format:	Article
Language:	English
Published:	University of Modena and Reggio Emilia 2014-06-01
Series:	Invertebrate Survival Journal
Subjects:	antimicrobial peptide Anemonia sulcata ATX II neurotoxin Micrococcus lysodeikticus
Online Access:	https://isj02.unimore.it/index.php/ISJ/article/view/319

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spelling	doaj-afe770a084314e38a7a2260c8d04a75a2020-12-02T18:43:53ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2014-06-01111First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)M R Trapani0M G Parisi1M Toubiana2L Coquet3T Jouenne4P Roch5M Cammarata6Marine Immunobiology Laboratory, Department of Biological, Chemical, Pharmaceutical Science and Technology, University of Palermo, Palermo, ItalyMarine Immunobiology Laboratory, Department of Biological, Chemical, Pharmaceutical Science and Technology, University of Palermo, Palermo, ItalyEcologie des Systèmes Marins Côtiers, CNRS-Université Montpellier 2, place E. Bataillon, Montpellier, FrancePlateforme de Protéomique PISSARO, CNRS-Université de Rouen, Place E. Blondel, Mont-Saint-Aignan, FrancePlateforme de Protéomique PISSARO, CNRS-Université de Rouen, Place E. Blondel, Mont-Saint-Aignan, FranceEcologie des Systèmes Marins Côtiers, CNRS-Université Montpellier 2, place E. Bataillon, Montpellier, FranceMarine Immunobiology Laboratory, Department of Biological, Chemical, Pharmaceutical Science and Technology, University of Palermo, Palermo, Italy We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule from tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and as antibacterial. https://isj02.unimore.it/index.php/ISJ/article/view/319antimicrobial peptideAnemonia sulcataATX IIneurotoxinMicrococcus lysodeikticus
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	M R Trapani M G Parisi M Toubiana L Coquet T Jouenne P Roch M Cammarata
spellingShingle	M R Trapani M G Parisi M Toubiana L Coquet T Jouenne P Roch M Cammarata First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria) Invertebrate Survival Journal antimicrobial peptide Anemonia sulcata ATX II neurotoxin Micrococcus lysodeikticus
author_facet	M R Trapani M G Parisi M Toubiana L Coquet T Jouenne P Roch M Cammarata
author_sort	M R Trapani
title	First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)
title_short	First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)
title_full	First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)
title_fullStr	First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)
title_full_unstemmed	First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)
title_sort	first evidence of antimicrobial activity of neurotoxin 2 from anemonia sulcata (cnidaria)
publisher	University of Modena and Reggio Emilia
series	Invertebrate Survival Journal
issn	1824-307X
publishDate	2014-06-01
description	We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule from tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and as antibacterial.
topic	antimicrobial peptide Anemonia sulcata ATX II neurotoxin Micrococcus lysodeikticus
url	https://isj02.unimore.it/index.php/ISJ/article/view/319
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First evidence of antimicrobial activity of neurotoxin 2 from Anemonia sulcata (Cnidaria)

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