Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum
Abstract Background Tannases are tannin-degrading enzymes that have been described in fungi and bacteria as an adaptative mechanism to overcome the stress conditions associated with the presence of these phenolic compounds. Results We have identified and expressed in E. coli a tannase from the oral...
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doaj-afee518bd93e4bb29900470a39f9c9ce2020-11-24T23:48:33ZengBMCMicrobial Cell Factories1475-28592018-02-0117111010.1186/s12934-018-0880-4Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphumJulen Tomás-Cortázar0Laura Plaza-Vinuesa1Blanca de las Rivas2José Luis Lavín3Diego Barriales4Leticia Abecia5José Miguel Mancheño6Ana M. Aransay7Rosario Muñoz8Juan Anguita9Héctor Rodríguez10Macrophage and Tick Vaccine Laboratory, CIC bioGUNELaboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN)-Consejo Superior de Investigaciones Científicas (CSIC)Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN)-Consejo Superior de Investigaciones Científicas (CSIC)Genome Analysis Platform, CIC bioGUNEMacrophage and Tick Vaccine Laboratory, CIC bioGUNEMacrophage and Tick Vaccine Laboratory, CIC bioGUNEDepartamento de Cristalografía y Biología Estructural, Instituto de Química-Física “Rocasolano” (IQFR-CSIC)Genome Analysis Platform, CIC bioGUNELaboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN)-Consejo Superior de Investigaciones Científicas (CSIC)Macrophage and Tick Vaccine Laboratory, CIC bioGUNEMacrophage and Tick Vaccine Laboratory, CIC bioGUNEAbstract Background Tannases are tannin-degrading enzymes that have been described in fungi and bacteria as an adaptative mechanism to overcome the stress conditions associated with the presence of these phenolic compounds. Results We have identified and expressed in E. coli a tannase from the oral microbiota member Fusobacterium nucleatum subs. polymorphum (TanBFnp). TanBFnp is the first tannase identified in an oral pathogen. Sequence analyses revealed that it is closely related to other bacterial tannases. The enzyme exhibits biochemical properties that make it an interesting target for industrial use. TanBFnp has one of the highest specific activities of all bacterial tannases described to date and shows optimal biochemical properties such as a high thermal stability: the enzyme keeps 100% of its activity after prolonged incubations at different temperatures up to 45 °C. TanBFnp also shows a wide temperature range of activity, maintaining above 80% of its maximum activity between 22 and 55 °C. The use of a panel of 27 esters of phenolic acids demonstrated activity of TanBFnp only against esters of gallic and protocatechuic acid, including tannic acid, gallocatechin gallate and epigallocatechin gallate. Overall, TanBFnp possesses biochemical properties that make the enzyme potentially useful in biotechnological applications. Conclusions We have identified and characterized a metabolic enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum. The biochemical properties of TanBFnp suggest that it has a major role in the breakdown of complex food tannins during oral processing. Our results also provide some clues regarding its possible participation on bacterial survival in the oral cavity. Furthermore, the characteristics of this enzyme make it of potential interest for industrial use.http://link.springer.com/article/10.1186/s12934-018-0880-4Phenolics biotransformationTannaseIndustrialFusobacteriumOral pathogen |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julen Tomás-Cortázar Laura Plaza-Vinuesa Blanca de las Rivas José Luis Lavín Diego Barriales Leticia Abecia José Miguel Mancheño Ana M. Aransay Rosario Muñoz Juan Anguita Héctor Rodríguez |
spellingShingle |
Julen Tomás-Cortázar Laura Plaza-Vinuesa Blanca de las Rivas José Luis Lavín Diego Barriales Leticia Abecia José Miguel Mancheño Ana M. Aransay Rosario Muñoz Juan Anguita Héctor Rodríguez Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum Microbial Cell Factories Phenolics biotransformation Tannase Industrial Fusobacterium Oral pathogen |
author_facet |
Julen Tomás-Cortázar Laura Plaza-Vinuesa Blanca de las Rivas José Luis Lavín Diego Barriales Leticia Abecia José Miguel Mancheño Ana M. Aransay Rosario Muñoz Juan Anguita Héctor Rodríguez |
author_sort |
Julen Tomás-Cortázar |
title |
Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum |
title_short |
Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum |
title_full |
Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum |
title_fullStr |
Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum |
title_full_unstemmed |
Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum |
title_sort |
identification of a highly active tannase enzyme from the oral pathogen fusobacterium nucleatum subsp. polymorphum |
publisher |
BMC |
series |
Microbial Cell Factories |
issn |
1475-2859 |
publishDate |
2018-02-01 |
description |
Abstract Background Tannases are tannin-degrading enzymes that have been described in fungi and bacteria as an adaptative mechanism to overcome the stress conditions associated with the presence of these phenolic compounds. Results We have identified and expressed in E. coli a tannase from the oral microbiota member Fusobacterium nucleatum subs. polymorphum (TanBFnp). TanBFnp is the first tannase identified in an oral pathogen. Sequence analyses revealed that it is closely related to other bacterial tannases. The enzyme exhibits biochemical properties that make it an interesting target for industrial use. TanBFnp has one of the highest specific activities of all bacterial tannases described to date and shows optimal biochemical properties such as a high thermal stability: the enzyme keeps 100% of its activity after prolonged incubations at different temperatures up to 45 °C. TanBFnp also shows a wide temperature range of activity, maintaining above 80% of its maximum activity between 22 and 55 °C. The use of a panel of 27 esters of phenolic acids demonstrated activity of TanBFnp only against esters of gallic and protocatechuic acid, including tannic acid, gallocatechin gallate and epigallocatechin gallate. Overall, TanBFnp possesses biochemical properties that make the enzyme potentially useful in biotechnological applications. Conclusions We have identified and characterized a metabolic enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum. The biochemical properties of TanBFnp suggest that it has a major role in the breakdown of complex food tannins during oral processing. Our results also provide some clues regarding its possible participation on bacterial survival in the oral cavity. Furthermore, the characteristics of this enzyme make it of potential interest for industrial use. |
topic |
Phenolics biotransformation Tannase Industrial Fusobacterium Oral pathogen |
url |
http://link.springer.com/article/10.1186/s12934-018-0880-4 |
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