Genetic divergence in Eucalyptus spp. clones by molecular RAPD markers

• Main Authors: Bruno Ettore Pavan, Rinaldo César de Paula, Janete Apparecida Desidério Sena, César Augusto Valencise Bonine, Edimar Aparecido Scarpinatti
• Format: Article
• Language: English
• Published: Brazilian Society of Plant Breeding 2006-01-01
• Series: Crop Breeding and Applied Biotechnology
• Subjects: Dissimilarity; clustering analysis; eucalypt; RAPD.
• Online Access: http://www.sbmp.org.br/cbab/siscbab/uploads/bd6ba09c-52db-224d.pdf

The genetic divergence in 20 Eucalyptus spp. clones was evaluated by multivariate techniques based on 167RAPD markers, of which 155 were polymorphic and 12 monomorphic. The measures of genetic distances were obtained by thearithmetic complement of the coefficients of Jaccard and of Sorenso-Nei and Li and evaluated by the hierarchical methods ofSingle Linkage clustering and Unweighted Pair Group Method with Arithmetic Mean (UPGMA). Independent of the dissimilaritycoefficient, the greatest divergence was found between clones 7 and 17 and the smallest between the clones 11 and 14. Cloneclustering was little influenced by the applied procedure so that, adopting the same percentage of divergence, the UPGMAidentified two groups less for the coefficient of Sorenso-Nei and Li. The clones evidenced considerable genetic divergence,which is partly associated to the origin of the study material. The clusters formed by the UPGMA clustering algorithmassociated to the arithmetic complement of Jaccard were most consistent.