Disparate Metabolomic Responses to Fructose Consumption between Different Mouse Strains and the Role of Gut Microbiota

Disparate Metabolomic Responses to Fructose Consumption between Different Mouse Strains and the Role of Gut Microbiota

High fructose consumption has been linked to metabolic syndrome, yet the fructose-induced phenotypes, gene expression, and gut microbiota alterations are distinct between mouse strains. In this study, we aim to investigate how fructose consumption shapes the metabolomic profiles of mice with differe...

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Main Authors: In Sook Ahn, Justin Yoon, Graciel Diamante, Peter Cohn, Cholsoon Jang, Xia Yang
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Metabolites
Subjects:
fructose
metabolomics
gut microbiota
genetic background
personalized disease risk
metabolic syndrome
Online Access:https://www.mdpi.com/2218-1989/11/6/342
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spelling doaj-affd54093f8e48538196835f706814742021-06-01T01:10:27ZengMDPI AGMetabolites2218-19892021-05-011134234210.3390/metabo11060342Disparate Metabolomic Responses to Fructose Consumption between Different Mouse Strains and the Role of Gut MicrobiotaIn Sook Ahn0Justin Yoon1Graciel Diamante2Peter Cohn3Cholsoon Jang4Xia Yang5Department of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USADepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USADepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USADepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USADepartment of Biological Chemistry, University of California, Irvine, CA 92697, USADepartment of Integrative Biology and Physiology, University of California, Los Angeles, CA 90095, USAHigh fructose consumption has been linked to metabolic syndrome, yet the fructose-induced phenotypes, gene expression, and gut microbiota alterations are distinct between mouse strains. In this study, we aim to investigate how fructose consumption shapes the metabolomic profiles of mice with different genetic background and microbiome. We used fructose-sensitive DBA/2J (DBA) and fructose-resistant C57BL/6J (B6) mice given 8% fructose or regular water for 12 weeks. Plasma and fecal metabolites were profiled using a liquid chromatography-tandem mass spectrometry based global metabolomic approach. We found that the baseline metabolomic profiles were different between DBA and B6 mice, particularly plasma metabolites involved in lipid metabolism and fecal metabolites related to dipeptide/amino acid metabolism. In response to fructose, DBA mice showed a distinct decrease of plasma branched chain fatty acids with concordantly increased branched chain amino acids, which were correlated with adiposity; B6 mice had significantly increased plasma cholesterol and total bile acids, accompanied by decreased fecal levels of farnesoid X receptor antagonist tauro-β-muricholate, which were correlated with fructose-responsive bacteria <i>Dehalobacterium</i>, <i>Magibacteriaceae</i>, and/or <i>Akkermansia</i>. Our results demonstrate that baseline metabolomic profiles differ and respond differentially to fructose between mice with different genetic background and gut microbiota, which may play a role in individualized risks to fructose-induced metabolic syndrome.https://www.mdpi.com/2218-1989/11/6/342fructosemetabolomicsgut microbiotagenetic backgroundpersonalized disease riskmetabolic syndrome
collection DOAJ
language English
format Article
sources DOAJ
author In Sook Ahn
Justin Yoon
Graciel Diamante
Peter Cohn
Cholsoon Jang
Xia Yang
spellingShingle In Sook Ahn
Justin Yoon
Graciel Diamante
Peter Cohn
Cholsoon Jang
Xia Yang
Disparate Metabolomic Responses to Fructose Consumption between Different Mouse Strains and the Role of Gut Microbiota
Metabolites
fructose
metabolomics
gut microbiota
genetic background
personalized disease risk
metabolic syndrome
author_facet In Sook Ahn
Justin Yoon
Graciel Diamante
Peter Cohn
Cholsoon Jang
Xia Yang
author_sort In Sook Ahn
title Disparate Metabolomic Responses to Fructose Consumption between Different Mouse Strains and the Role of Gut Microbiota
title_short Disparate Metabolomic Responses to Fructose Consumption between Different Mouse Strains and the Role of Gut Microbiota
title_full Disparate Metabolomic Responses to Fructose Consumption between Different Mouse Strains and the Role of Gut Microbiota
title_fullStr Disparate Metabolomic Responses to Fructose Consumption between Different Mouse Strains and the Role of Gut Microbiota
title_full_unstemmed Disparate Metabolomic Responses to Fructose Consumption between Different Mouse Strains and the Role of Gut Microbiota
title_sort disparate metabolomic responses to fructose consumption between different mouse strains and the role of gut microbiota
publisher MDPI AG
series Metabolites
issn 2218-1989
publishDate 2021-05-01
description High fructose consumption has been linked to metabolic syndrome, yet the fructose-induced phenotypes, gene expression, and gut microbiota alterations are distinct between mouse strains. In this study, we aim to investigate how fructose consumption shapes the metabolomic profiles of mice with different genetic background and microbiome. We used fructose-sensitive DBA/2J (DBA) and fructose-resistant C57BL/6J (B6) mice given 8% fructose or regular water for 12 weeks. Plasma and fecal metabolites were profiled using a liquid chromatography-tandem mass spectrometry based global metabolomic approach. We found that the baseline metabolomic profiles were different between DBA and B6 mice, particularly plasma metabolites involved in lipid metabolism and fecal metabolites related to dipeptide/amino acid metabolism. In response to fructose, DBA mice showed a distinct decrease of plasma branched chain fatty acids with concordantly increased branched chain amino acids, which were correlated with adiposity; B6 mice had significantly increased plasma cholesterol and total bile acids, accompanied by decreased fecal levels of farnesoid X receptor antagonist tauro-β-muricholate, which were correlated with fructose-responsive bacteria <i>Dehalobacterium</i>, <i>Magibacteriaceae</i>, and/or <i>Akkermansia</i>. Our results demonstrate that baseline metabolomic profiles differ and respond differentially to fructose between mice with different genetic background and gut microbiota, which may play a role in individualized risks to fructose-induced metabolic syndrome.
topic fructose
metabolomics
gut microbiota
genetic background
personalized disease risk
metabolic syndrome
url https://www.mdpi.com/2218-1989/11/6/342
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AT justinyoon disparatemetabolomicresponsestofructoseconsumptionbetweendifferentmousestrainsandtheroleofgutmicrobiota
AT gracieldiamante disparatemetabolomicresponsestofructoseconsumptionbetweendifferentmousestrainsandtheroleofgutmicrobiota
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AT xiayang disparatemetabolomicresponsestofructoseconsumptionbetweendifferentmousestrainsandtheroleofgutmicrobiota
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