A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicity
The self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of С60 fullerene, Cis and their...
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| Series: | Beilstein Journal of Nanotechnology |
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| Online Access: | https://doi.org/10.3762/bjnano.8.149 |
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doaj-affe3dc630254a148c0c2b0861994d2b2020-11-25T01:26:13ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862017-07-01811494150110.3762/bjnano.8.1492190-4286-8-149A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicitySvitlana Prylutska0Svitlana Politenkova1Kateryna Afanasieva2Volodymyr Korolovych3Kateryna Bogutska4Andriy Sivolob5Larysa Skivka6Maxim Evstigneev7Viktor Kostjukov8Yuriy Prylutskyy9Uwe Ritter10Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineSchool of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, USATaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineBelgorod State University, Pobedy Str. 85, 308015 Belgorod, RussiaDepartment of Physics, Sevastopol State University, Sevastopol 299053, CrimeaTaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineTechnical University of Ilmenau, Institute of Chemistry and Biotechnology, Weimarer Str., 25, 98693 Ilmenau, GermanyThe self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of С60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. The cytotoxicity of the mentioned compounds was estimated by Annexin V/PI double staining followed by flow cytometry. The results clearly demonstrate that water-soluble C60 fullerene nanoparticles (0.1 mg/mL) do not induce DNA strand breaks in normal and transformed cells. C60 fullerene in the mixture with Cis does not influence genotoxic Cis activity in vitro, affects the cell-death mode in treated resting human lymphocytes and reduces the fraction of necrotic cells.https://doi.org/10.3762/bjnano.8.149atomic force microscopyC60 fullerenecisplatincomet assaycomputer simulationdynamic light scatteringflow cytometryhuman lymphocytestoxicity in vitro |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Svitlana Prylutska Svitlana Politenkova Kateryna Afanasieva Volodymyr Korolovych Kateryna Bogutska Andriy Sivolob Larysa Skivka Maxim Evstigneev Viktor Kostjukov Yuriy Prylutskyy Uwe Ritter |
| spellingShingle |
Svitlana Prylutska Svitlana Politenkova Kateryna Afanasieva Volodymyr Korolovych Kateryna Bogutska Andriy Sivolob Larysa Skivka Maxim Evstigneev Viktor Kostjukov Yuriy Prylutskyy Uwe Ritter A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicity Beilstein Journal of Nanotechnology atomic force microscopy C60 fullerene cisplatin comet assay computer simulation dynamic light scattering flow cytometry human lymphocytes toxicity in vitro |
| author_facet |
Svitlana Prylutska Svitlana Politenkova Kateryna Afanasieva Volodymyr Korolovych Kateryna Bogutska Andriy Sivolob Larysa Skivka Maxim Evstigneev Viktor Kostjukov Yuriy Prylutskyy Uwe Ritter |
| author_sort |
Svitlana Prylutska |
| title |
A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicity |
| title_short |
A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicity |
| title_full |
A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicity |
| title_fullStr |
A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicity |
| title_full_unstemmed |
A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicity |
| title_sort |
nanocomplex of c60 fullerene with cisplatin: design, characterization and toxicity |
| publisher |
Beilstein-Institut |
| series |
Beilstein Journal of Nanotechnology |
| issn |
2190-4286 |
| publishDate |
2017-07-01 |
| description |
The self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of С60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. The cytotoxicity of the mentioned compounds was estimated by Annexin V/PI double staining followed by flow cytometry. The results clearly demonstrate that water-soluble C60 fullerene nanoparticles (0.1 mg/mL) do not induce DNA strand breaks in normal and transformed cells. C60 fullerene in the mixture with Cis does not influence genotoxic Cis activity in vitro, affects the cell-death mode in treated resting human lymphocytes and reduces the fraction of necrotic cells. |
| topic |
atomic force microscopy C60 fullerene cisplatin comet assay computer simulation dynamic light scattering flow cytometry human lymphocytes toxicity in vitro |
| url |
https://doi.org/10.3762/bjnano.8.149 |
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