Bioactive Lipids of Marine Microalga <i>Chlorococcum</i> sp. SABC 012504 with Anti-Inflammatory and Anti-thrombotic Activities
Microalgae are at the start of the food chain, and many are known producers of a significant amount of lipids with essential fatty acids. However, the bioactivity of microalgal lipids for anti-inflammatory and antithrombotic activities have rarely been investigated. Therefore, for a sustainable sour...
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doaj-b007e12389824ccead8956f9ffbf96a92021-01-11T00:00:33ZengMDPI AGMarine Drugs1660-33972021-01-0119282810.3390/md19010028Bioactive Lipids of Marine Microalga <i>Chlorococcum</i> sp. SABC 012504 with Anti-Inflammatory and Anti-thrombotic ActivitiesKatie Shiels0Alexandros Tsoupras1Ronan Lordan2Constantina Nasopoulou3Ioannis Zabetakis4Patrick Murray5Sushanta Kumar Saha6Shannon Applied Biotechnology Centre, Limerick Institute of Technology, Moylish Park, V94 E8YF Limerick, IrelandDepartment of Biological Sciences, University of Limerick, V94 T9PX Limerick, IrelandInstitute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USADepartment of Food Science and Nutrition, School of the Environment, University of the Aegean, GR 81400 Myrina, Lemnos, GreeceDepartment of Biological Sciences, University of Limerick, V94 T9PX Limerick, IrelandShannon Applied Biotechnology Centre, Limerick Institute of Technology, Moylish Park, V94 E8YF Limerick, IrelandShannon Applied Biotechnology Centre, Limerick Institute of Technology, Moylish Park, V94 E8YF Limerick, IrelandMicroalgae are at the start of the food chain, and many are known producers of a significant amount of lipids with essential fatty acids. However, the bioactivity of microalgal lipids for anti-inflammatory and antithrombotic activities have rarely been investigated. Therefore, for a sustainable source of the above bioactive lipids, the present study was undertaken. The total lipids of microalga <i>Chlorococcum</i> sp., isolated from the Irish coast, were fractionated into neutral-, glyco-, and phospho-lipids, and were tested in vitro for their anti-inflammatory and antithrombotic activities. All tested lipid fractions showed strong anti-platelet-activating factor (PAF) and antithrombin activities in human platelets (half maximal inhibitory concentration (IC<sub>50</sub>) values ranging ~25–200 μg of lipid) with the highest activities in glyco- and phospho-lipid fractions. The structural analysis of the bioactive lipid fraction-2 revealed the presence of specific sulfoquinovosyl diacylglycerols (SQDG) bioactive molecules and the HexCer-t36:2 (t18:1/18:1 and 18:2/18:0) cerebrosides with a phytosphingosine (4-hydrosphinganine) base, while fraction-3 contained bioactive phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules. These novel bioactive lipids of <i>Chlorococcum</i> sp. with putative health benefits may indicate that marine microalgae can be a sustainable alternative source for bioactive lipids production for food supplements and nutraceutical applications. However, further studies are required towards the commercial technology pathways development and biosafety analysis for the use of the microalga.https://www.mdpi.com/1660-3397/19/1/28marine microalga<i>Chlorococcum</i> sp.bioactive lipidanti-inflammatoryantithromboticplatelets |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katie Shiels Alexandros Tsoupras Ronan Lordan Constantina Nasopoulou Ioannis Zabetakis Patrick Murray Sushanta Kumar Saha |
spellingShingle |
Katie Shiels Alexandros Tsoupras Ronan Lordan Constantina Nasopoulou Ioannis Zabetakis Patrick Murray Sushanta Kumar Saha Bioactive Lipids of Marine Microalga <i>Chlorococcum</i> sp. SABC 012504 with Anti-Inflammatory and Anti-thrombotic Activities Marine Drugs marine microalga <i>Chlorococcum</i> sp. bioactive lipid anti-inflammatory antithrombotic platelets |
author_facet |
Katie Shiels Alexandros Tsoupras Ronan Lordan Constantina Nasopoulou Ioannis Zabetakis Patrick Murray Sushanta Kumar Saha |
author_sort |
Katie Shiels |
title |
Bioactive Lipids of Marine Microalga <i>Chlorococcum</i> sp. SABC 012504 with Anti-Inflammatory and Anti-thrombotic Activities |
title_short |
Bioactive Lipids of Marine Microalga <i>Chlorococcum</i> sp. SABC 012504 with Anti-Inflammatory and Anti-thrombotic Activities |
title_full |
Bioactive Lipids of Marine Microalga <i>Chlorococcum</i> sp. SABC 012504 with Anti-Inflammatory and Anti-thrombotic Activities |
title_fullStr |
Bioactive Lipids of Marine Microalga <i>Chlorococcum</i> sp. SABC 012504 with Anti-Inflammatory and Anti-thrombotic Activities |
title_full_unstemmed |
Bioactive Lipids of Marine Microalga <i>Chlorococcum</i> sp. SABC 012504 with Anti-Inflammatory and Anti-thrombotic Activities |
title_sort |
bioactive lipids of marine microalga <i>chlorococcum</i> sp. sabc 012504 with anti-inflammatory and anti-thrombotic activities |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2021-01-01 |
description |
Microalgae are at the start of the food chain, and many are known producers of a significant amount of lipids with essential fatty acids. However, the bioactivity of microalgal lipids for anti-inflammatory and antithrombotic activities have rarely been investigated. Therefore, for a sustainable source of the above bioactive lipids, the present study was undertaken. The total lipids of microalga <i>Chlorococcum</i> sp., isolated from the Irish coast, were fractionated into neutral-, glyco-, and phospho-lipids, and were tested in vitro for their anti-inflammatory and antithrombotic activities. All tested lipid fractions showed strong anti-platelet-activating factor (PAF) and antithrombin activities in human platelets (half maximal inhibitory concentration (IC<sub>50</sub>) values ranging ~25–200 μg of lipid) with the highest activities in glyco- and phospho-lipid fractions. The structural analysis of the bioactive lipid fraction-2 revealed the presence of specific sulfoquinovosyl diacylglycerols (SQDG) bioactive molecules and the HexCer-t36:2 (t18:1/18:1 and 18:2/18:0) cerebrosides with a phytosphingosine (4-hydrosphinganine) base, while fraction-3 contained bioactive phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules. These novel bioactive lipids of <i>Chlorococcum</i> sp. with putative health benefits may indicate that marine microalgae can be a sustainable alternative source for bioactive lipids production for food supplements and nutraceutical applications. However, further studies are required towards the commercial technology pathways development and biosafety analysis for the use of the microalga. |
topic |
marine microalga <i>Chlorococcum</i> sp. bioactive lipid anti-inflammatory antithrombotic platelets |
url |
https://www.mdpi.com/1660-3397/19/1/28 |
work_keys_str_mv |
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