Thymol has beneficial effects on the experimental model of ulcerative colitis
<strong>Objective:</strong> Thymol, a natural aromatic monoterpene phenol derived from thymus, possesses anti-inflammatory benefits. Here, we evaluated the potential of thymol therapy in improving an animal model of ulcerative colitis.<br /> <strong>Materials and Methods:<...
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Mashhad University of Medical Sciences
2019-10-01
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doaj-b00de81bdce6490db31019e6b50f8e4f2020-11-25T01:51:04ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492019-10-019653855010.22038/ajp.2019.1338313383Thymol has beneficial effects on the experimental model of ulcerative colitisPouriya Tahmasebi0Seyyed Meysam Abtahi froushani1Nahideh Afzale Ahangaran2Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.<strong>Objective:</strong> Thymol, a natural aromatic monoterpene phenol derived from thymus, possesses anti-inflammatory benefits. Here, we evaluated the potential of thymol therapy in improving an animal model of ulcerative colitis.<br /> <strong>Materials and Methods:</strong> Luminal instillation of acetic acid was used to induce colitis in male Wistar rats. Treatment groups daily received prednisolone (2 mg/kg, orally) or thymol (100 mg/kg, orally) for 10 consecutive days. Then, the rats were euthanized and tissue specimens were collected for evaluation of cyclooxygenase-2 (COX-2) expression by immunohistochemistry. Furthermore, the levels of total protein, nitric oxide, myeloperoxidase, malondialdehyde, IL-1, IL-6, and TNF-α were monitored in colonic homogenates. Eventually, the relative mRNA expression of <em>IκBα </em>and <em>NF-κBp65</em> was investigated using reverse-transcriptase PCR (RT-PCR) in colonic homogenates.<br /> <strong>Results:</strong> Both medications could reduce the mortality rate and the clinical scores of ulcerative colitis. The COX-2 expression was significantly reduced in the colons of thymol-treated animals compared to the prednisolone group. Also, the myeloperoxidase activity, nitric oxide level and malondialdehyde intensity were decreased in the colons of thymol-treated animals to a greater extent compared to the prednisolone group. Moreover, the total protein content of guts showed significant increases in the guts of thymol-treated animals in comparison to the prednisolone group. Nonetheless, thymol significantly reduced the levels of IL-6, and IL-1 compared to prednisolone. Both medications caused a significant decrease in the mRNA level of <em>NF-κBp65</em>, though the mRNA level of <em>IκBα</em> did not show significant changes between the groups.<br /> <strong>Conclusion:</strong> Thymol may be a promising agent to ameliorate ulcerative colitis.http://ajp.mums.ac.ir/article_13383_40841d5a95b496dfed87ec2636439839.pdfThymolAcetic-acidUlcerative colitisWistar rat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pouriya Tahmasebi Seyyed Meysam Abtahi froushani Nahideh Afzale Ahangaran |
spellingShingle |
Pouriya Tahmasebi Seyyed Meysam Abtahi froushani Nahideh Afzale Ahangaran Thymol has beneficial effects on the experimental model of ulcerative colitis Avicenna Journal of Phytomedicine Thymol Acetic-acid Ulcerative colitis Wistar rat |
author_facet |
Pouriya Tahmasebi Seyyed Meysam Abtahi froushani Nahideh Afzale Ahangaran |
author_sort |
Pouriya Tahmasebi |
title |
Thymol has beneficial effects on the experimental model of ulcerative colitis |
title_short |
Thymol has beneficial effects on the experimental model of ulcerative colitis |
title_full |
Thymol has beneficial effects on the experimental model of ulcerative colitis |
title_fullStr |
Thymol has beneficial effects on the experimental model of ulcerative colitis |
title_full_unstemmed |
Thymol has beneficial effects on the experimental model of ulcerative colitis |
title_sort |
thymol has beneficial effects on the experimental model of ulcerative colitis |
publisher |
Mashhad University of Medical Sciences |
series |
Avicenna Journal of Phytomedicine |
issn |
2228-7930 2228-7949 |
publishDate |
2019-10-01 |
description |
<strong>Objective:</strong> Thymol, a natural aromatic monoterpene phenol derived from thymus, possesses anti-inflammatory benefits. Here, we evaluated the potential of thymol therapy in improving an animal model of ulcerative colitis.<br /> <strong>Materials and Methods:</strong> Luminal instillation of acetic acid was used to induce colitis in male Wistar rats. Treatment groups daily received prednisolone (2 mg/kg, orally) or thymol (100 mg/kg, orally) for 10 consecutive days. Then, the rats were euthanized and tissue specimens were collected for evaluation of cyclooxygenase-2 (COX-2) expression by immunohistochemistry. Furthermore, the levels of total protein, nitric oxide, myeloperoxidase, malondialdehyde, IL-1, IL-6, and TNF-α were monitored in colonic homogenates. Eventually, the relative mRNA expression of <em>IκBα </em>and <em>NF-κBp65</em> was investigated using reverse-transcriptase PCR (RT-PCR) in colonic homogenates.<br /> <strong>Results:</strong> Both medications could reduce the mortality rate and the clinical scores of ulcerative colitis. The COX-2 expression was significantly reduced in the colons of thymol-treated animals compared to the prednisolone group. Also, the myeloperoxidase activity, nitric oxide level and malondialdehyde intensity were decreased in the colons of thymol-treated animals to a greater extent compared to the prednisolone group. Moreover, the total protein content of guts showed significant increases in the guts of thymol-treated animals in comparison to the prednisolone group. Nonetheless, thymol significantly reduced the levels of IL-6, and IL-1 compared to prednisolone. Both medications caused a significant decrease in the mRNA level of <em>NF-κBp65</em>, though the mRNA level of <em>IκBα</em> did not show significant changes between the groups.<br /> <strong>Conclusion:</strong> Thymol may be a promising agent to ameliorate ulcerative colitis. |
topic |
Thymol Acetic-acid Ulcerative colitis Wistar rat |
url |
http://ajp.mums.ac.ir/article_13383_40841d5a95b496dfed87ec2636439839.pdf |
work_keys_str_mv |
AT pouriyatahmasebi thymolhasbeneficialeffectsontheexperimentalmodelofulcerativecolitis AT seyyedmeysamabtahifroushani thymolhasbeneficialeffectsontheexperimentalmodelofulcerativecolitis AT nahidehafzaleahangaran thymolhasbeneficialeffectsontheexperimentalmodelofulcerativecolitis |
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