Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals
Abstract The microbiome of flowers (anthosphere) is an understudied compartment of the plant microbiome. Within the flower, petals represent a heterogeneous environment for microbes in terms of resources and environmental stress. Yet, little is known of drivers of structure and function of the epiph...
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doaj-b01c4908e0f6462996719be975a0992d2021-06-28T00:16:10ZengWileyMicrobiologyOpen2045-88272021-02-01101n/an/a10.1002/mbo3.1158Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petalsRebecca A. Hayes0Maria Rebolleda‐Gómez1Kristen Butela2Leah F. Cabo3Nevin Cullen4Nancy Kaufmann5Steffani O'Neill6Tia‐Lynn Ashman7Department of Biological Sciences University of Pittsburgh Pittsburgh PA USADepartment of Biological Sciences University of Pittsburgh Pittsburgh PA USADepartment of Biological Sciences University of Pittsburgh Pittsburgh PA USADepartment of Biological Sciences University of Pittsburgh Pittsburgh PA USADepartment of Biological Sciences University of Pittsburgh Pittsburgh PA USADepartment of Biological Sciences University of Pittsburgh Pittsburgh PA USADepartment of Biological Sciences University of Pittsburgh Pittsburgh PA USADepartment of Biological Sciences University of Pittsburgh Pittsburgh PA USAAbstract The microbiome of flowers (anthosphere) is an understudied compartment of the plant microbiome. Within the flower, petals represent a heterogeneous environment for microbes in terms of resources and environmental stress. Yet, little is known of drivers of structure and function of the epiphytic microbial community at the within‐petal scale. We characterized the petal microbiome in two co‐flowering plants that differ in the pattern of ultraviolet (UV) absorption along their petals. Bacterial communities were similar between plant hosts, with only rare phylogenetically distant species contributing to differences. The epiphyte community was highly culturable (75% of families) lending confidence in the spatially explicit isolation and characterization of bacteria. In one host, petals were heterogeneous in UV absorption along their length, and in these, there was a negative relationship between growth rate and position on the petal, as well as lower UV tolerance in strains isolated from the UV‐absorbing base than from UV reflecting tip. A similar pattern was not seen in microbes isolated from a second host whose petals had uniform patterning along their length. Across strains, the variation in carbon usage and chemical tolerance followed common phylogenetic patterns. This work highlights the value of petals for spatially explicit explorations of bacteria of the anthosphere.https://doi.org/10.1002/mbo3.1158Anthospherecommunity ecologyHelianthus tuberosusplant microbiomespatial structureVerbesina alternifolia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rebecca A. Hayes Maria Rebolleda‐Gómez Kristen Butela Leah F. Cabo Nevin Cullen Nancy Kaufmann Steffani O'Neill Tia‐Lynn Ashman |
spellingShingle |
Rebecca A. Hayes Maria Rebolleda‐Gómez Kristen Butela Leah F. Cabo Nevin Cullen Nancy Kaufmann Steffani O'Neill Tia‐Lynn Ashman Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals MicrobiologyOpen Anthosphere community ecology Helianthus tuberosus plant microbiome spatial structure Verbesina alternifolia |
author_facet |
Rebecca A. Hayes Maria Rebolleda‐Gómez Kristen Butela Leah F. Cabo Nevin Cullen Nancy Kaufmann Steffani O'Neill Tia‐Lynn Ashman |
author_sort |
Rebecca A. Hayes |
title |
Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals |
title_short |
Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals |
title_full |
Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals |
title_fullStr |
Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals |
title_full_unstemmed |
Spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals |
title_sort |
spatially explicit depiction of a floral epiphytic bacterial community reveals role for environmental filtering within petals |
publisher |
Wiley |
series |
MicrobiologyOpen |
issn |
2045-8827 |
publishDate |
2021-02-01 |
description |
Abstract The microbiome of flowers (anthosphere) is an understudied compartment of the plant microbiome. Within the flower, petals represent a heterogeneous environment for microbes in terms of resources and environmental stress. Yet, little is known of drivers of structure and function of the epiphytic microbial community at the within‐petal scale. We characterized the petal microbiome in two co‐flowering plants that differ in the pattern of ultraviolet (UV) absorption along their petals. Bacterial communities were similar between plant hosts, with only rare phylogenetically distant species contributing to differences. The epiphyte community was highly culturable (75% of families) lending confidence in the spatially explicit isolation and characterization of bacteria. In one host, petals were heterogeneous in UV absorption along their length, and in these, there was a negative relationship between growth rate and position on the petal, as well as lower UV tolerance in strains isolated from the UV‐absorbing base than from UV reflecting tip. A similar pattern was not seen in microbes isolated from a second host whose petals had uniform patterning along their length. Across strains, the variation in carbon usage and chemical tolerance followed common phylogenetic patterns. This work highlights the value of petals for spatially explicit explorations of bacteria of the anthosphere. |
topic |
Anthosphere community ecology Helianthus tuberosus plant microbiome spatial structure Verbesina alternifolia |
url |
https://doi.org/10.1002/mbo3.1158 |
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