MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer
MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. Each step of their production and maturation has to be strictly regulated, as any disruption of control mechanisms may lead to cancer. Thus, we have measured the expression of 19 genes involved in miRN...
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doaj-b01ea035d464473fb22dd2c0bfbb334e2020-11-25T02:43:19ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-09-012018446010.3390/ijms20184460ijms20184460MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal CancerPetra Vychytilova-Faltejskova0Alena Svobodova Kovarikova1Tomas Grolich2Vladimir Prochazka3Katerina Slaba4Tana Machackova5Jana Halamkova6Marek Svoboda7Zdenek Kala8Igor Kiss9Ondrej Slaby10Central European Institute of Technology, Masaryk University, Kamenice 753/5, 62500 Brno, Czech RepublicCentral European Institute of Technology, Masaryk University, Kamenice 753/5, 62500 Brno, Czech RepublicDepartment of Surgery, University Hospital Brno, Jihlavska 340/20, 62500 Brno, Czech RepublicDepartment of Surgery, University Hospital Brno, Jihlavska 340/20, 62500 Brno, Czech RepublicFaculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech RepublicCentral European Institute of Technology, Masaryk University, Kamenice 753/5, 62500 Brno, Czech RepublicDepartment of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Faculty of Medicine, Masaryk University, 62500 Brno, Czech RepublicCentral European Institute of Technology, Masaryk University, Kamenice 753/5, 62500 Brno, Czech RepublicDepartment of Surgery, University Hospital Brno, Jihlavska 340/20, 62500 Brno, Czech RepublicFaculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech RepublicCentral European Institute of Technology, Masaryk University, Kamenice 753/5, 62500 Brno, Czech RepublicMicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. Each step of their production and maturation has to be strictly regulated, as any disruption of control mechanisms may lead to cancer. Thus, we have measured the expression of 19 genes involved in miRNAs biogenesis pathway in tumor tissues of 239 colorectal cancer (CRC) patients, 17 CRC patients with liver metastases and 239 adjacent tissues using real-time PCR. Subsequently, the expression of analyzed genes was correlated with the clinical-pathological features as well as with the survival of patients. In total, significant over-expression of all analyzed genes was observed in tumor tissues as well as in liver metastases except for LIN28A/B. Furthermore, it was shown that the deregulated levels of some of the analyzed genes significantly correlate with tumor stage, grade, location, size and lymph node positivity. Finally, high levels of DROSHA and TARBP2 were associated with shorter disease-free survival, while the over-expression of XPO5, TNRC6A and DDX17 was detected in tissues of patients with shorter overall survival and poor prognosis. Our data indicate that changed levels of miRNA biogenesis genes may contribute to origin as well as progression of CRC; thus, these molecules could serve as potential therapeutic targets.https://www.mdpi.com/1422-0067/20/18/4460microRNAbiogenesiscolorectal cancerdisease-free survivaloverall survivalRT-qPCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Petra Vychytilova-Faltejskova Alena Svobodova Kovarikova Tomas Grolich Vladimir Prochazka Katerina Slaba Tana Machackova Jana Halamkova Marek Svoboda Zdenek Kala Igor Kiss Ondrej Slaby |
spellingShingle |
Petra Vychytilova-Faltejskova Alena Svobodova Kovarikova Tomas Grolich Vladimir Prochazka Katerina Slaba Tana Machackova Jana Halamkova Marek Svoboda Zdenek Kala Igor Kiss Ondrej Slaby MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer International Journal of Molecular Sciences microRNA biogenesis colorectal cancer disease-free survival overall survival RT-qPCR |
author_facet |
Petra Vychytilova-Faltejskova Alena Svobodova Kovarikova Tomas Grolich Vladimir Prochazka Katerina Slaba Tana Machackova Jana Halamkova Marek Svoboda Zdenek Kala Igor Kiss Ondrej Slaby |
author_sort |
Petra Vychytilova-Faltejskova |
title |
MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer |
title_short |
MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer |
title_full |
MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer |
title_fullStr |
MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer |
title_full_unstemmed |
MicroRNA Biogenesis Pathway Genes Are Deregulated in Colorectal Cancer |
title_sort |
microrna biogenesis pathway genes are deregulated in colorectal cancer |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-09-01 |
description |
MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. Each step of their production and maturation has to be strictly regulated, as any disruption of control mechanisms may lead to cancer. Thus, we have measured the expression of 19 genes involved in miRNAs biogenesis pathway in tumor tissues of 239 colorectal cancer (CRC) patients, 17 CRC patients with liver metastases and 239 adjacent tissues using real-time PCR. Subsequently, the expression of analyzed genes was correlated with the clinical-pathological features as well as with the survival of patients. In total, significant over-expression of all analyzed genes was observed in tumor tissues as well as in liver metastases except for LIN28A/B. Furthermore, it was shown that the deregulated levels of some of the analyzed genes significantly correlate with tumor stage, grade, location, size and lymph node positivity. Finally, high levels of DROSHA and TARBP2 were associated with shorter disease-free survival, while the over-expression of XPO5, TNRC6A and DDX17 was detected in tissues of patients with shorter overall survival and poor prognosis. Our data indicate that changed levels of miRNA biogenesis genes may contribute to origin as well as progression of CRC; thus, these molecules could serve as potential therapeutic targets. |
topic |
microRNA biogenesis colorectal cancer disease-free survival overall survival RT-qPCR |
url |
https://www.mdpi.com/1422-0067/20/18/4460 |
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