| Summary: | The possibility of dihaploid development from tetrahaploid anthers in strawberry has been tested in culture. From the total number of 4635 anthers taken from 57 tetrahaploid plants (secondary cycle of anther culture) and 4042 anthers from 7 octoploid varieties - 119 plants were obtained. The success depended on the growing conditions of donor plants (field conditions appeared better) and plant genotypes (tetrahaploids previously regenerated in vitro were better donors). The majority of plants regenerated from tetrahaploid anther callus (54.6%) were mixoploids. The remaining plants were polyploids from 2n=4x=28 to 2n=8x=56. Only 3 mixoploid individuals had lower than 2n=4x=28 number of chromosomes. Plants regenerated from anther callus were capable of developing seeds and fruits. Seed germination was rather low and fruit yield varied. Still, it was possible to select a group of 12 plants, possibly of somaclonal origin, fit for further breeding.
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