Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method
Xue-Feng Zhao1,2, Xiao-Dong Li3, Yun-Qing Kang4, Quan Yuan1,21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People's Republic of China; 2West China School of Stomatology, Sichuan University, Chengdu, People's Republic of China; 3Affiliated Hospital of St...
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2011-07-01
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doaj-b03bc77830ee4f42b17d444d237cb97a2020-11-24T22:49:05ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132011-07-012011default13851390Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free methodZhao XFLi XDKang YQYuan QXue-Feng Zhao1,2, Xiao-Dong Li3, Yun-Qing Kang4, Quan Yuan1,21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People's Republic of China; 2West China School of Stomatology, Sichuan University, Chengdu, People's Republic of China; 3Affiliated Hospital of Stomatology and College of Stomatology, Chongqing Medical University, Chongqing, People's Republic of China; 4College of Materials Science and Engineering, Sichuan University, Chengdu, People's Republic of ChinaAbstract: A porous poly(L-lactic acid)/ß-tricalcium phosphate (PLLA/ß-TCP) composite scaffold was fabricated using a novel technique comprising powder mixing, compression molding, low-temperature treatment, and particulate leaching without any organic solvent. The effect of this scaffold on osteoblast proliferation and differentiation was evaluated in vitro. The fabricated scaffold had a homogeneously interconnected porous structure with a porosity of 70% and compressive strength of 1.35 MPa. The methylthiazol tetrazolium values and alkaline phosphatase (ALP) activity of osteoblasts seeded on the solvent-free scaffold were significant higher than those of the control. Using real-time PCR, gene expressions of ALP, osteocalcin, and type 1 collagen were shown to be upregulated. As the method does not use any organic solvent, it eliminates problems associated with organic solvent residue and therefore improves the cell compatibility. It has a promising potential for the preparation of porous scaffold for bone tissue engineering.Keywords: biocompatibility, biomaterials, composites, poly(L-lactic acid), ß-tricalcium phosphatehttp://www.dovepress.com/improved-biocompatibility-of-novel-polyl-lactic-acidszlig-tricalcium-p-a7794 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhao XF Li XD Kang YQ Yuan Q |
spellingShingle |
Zhao XF Li XD Kang YQ Yuan Q Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method International Journal of Nanomedicine |
author_facet |
Zhao XF Li XD Kang YQ Yuan Q |
author_sort |
Zhao XF |
title |
Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method |
title_short |
Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method |
title_full |
Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method |
title_fullStr |
Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method |
title_full_unstemmed |
Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method |
title_sort |
improved biocompatibility of novel poly(l-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method |
publisher |
Dove Medical Press |
series |
International Journal of Nanomedicine |
issn |
1176-9114 1178-2013 |
publishDate |
2011-07-01 |
description |
Xue-Feng Zhao1,2, Xiao-Dong Li3, Yun-Qing Kang4, Quan Yuan1,21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People's Republic of China; 2West China School of Stomatology, Sichuan University, Chengdu, People's Republic of China; 3Affiliated Hospital of Stomatology and College of Stomatology, Chongqing Medical University, Chongqing, People's Republic of China; 4College of Materials Science and Engineering, Sichuan University, Chengdu, People's Republic of ChinaAbstract: A porous poly(L-lactic acid)/ß-tricalcium phosphate (PLLA/ß-TCP) composite scaffold was fabricated using a novel technique comprising powder mixing, compression molding, low-temperature treatment, and particulate leaching without any organic solvent. The effect of this scaffold on osteoblast proliferation and differentiation was evaluated in vitro. The fabricated scaffold had a homogeneously interconnected porous structure with a porosity of 70% and compressive strength of 1.35 MPa. The methylthiazol tetrazolium values and alkaline phosphatase (ALP) activity of osteoblasts seeded on the solvent-free scaffold were significant higher than those of the control. Using real-time PCR, gene expressions of ALP, osteocalcin, and type 1 collagen were shown to be upregulated. As the method does not use any organic solvent, it eliminates problems associated with organic solvent residue and therefore improves the cell compatibility. It has a promising potential for the preparation of porous scaffold for bone tissue engineering.Keywords: biocompatibility, biomaterials, composites, poly(L-lactic acid), ß-tricalcium phosphate |
url |
http://www.dovepress.com/improved-biocompatibility-of-novel-polyl-lactic-acidszlig-tricalcium-p-a7794 |
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