Structure of bacterial chromosome: An analysis of DNA-protein interactions in vivo
According to recent reports, bacterial chromosomes exhibit a hierarchical organization. The number of proteins that bind DNA are responsible for local and global organization of the DNA ensuring proper chromosome compaction. Advanced molecular biology techniques combined with high-throughput DNA seq...
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Index Copernicus International S.A.
2017-12-01
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doaj-b03eb269242c42cd9748b798c2c3ff652020-11-24T23:40:58ZengIndex Copernicus International S.A.Postępy Higieny i Medycyny Doświadczalnej0032-54491732-26932017-12-01711005101410.5604/01.3001.0010.669601.3001.0010.6696Structure of bacterial chromosome: An analysis of DNA-protein interactions in vivoJoanna Hołówka0Małgorzata Płachetka1Instytut Immunologii i Terapii Doświadczalnej im. Ludwika Hirszfelda Polskiej Akademii Nauk we WrocławiuUniwersytet Wrocławski, Wydział BiotechnologiiAccording to recent reports, bacterial chromosomes exhibit a hierarchical organization. The number of proteins that bind DNA are responsible for local and global organization of the DNA ensuring proper chromosome compaction. Advanced molecular biology techniques combined with high-throughput DNA sequencing methods allow a precise analysis of bacterial chromosome structures on a local and global scale. Methods such as in vivo footprinting and ChIP-seq allow to map binding sites of analyzed proteins in certain chromosomal regions or along the whole chromosome while analysis of the spatial interactions on global scale could be performed by 3C techniques. Additional insight into complex structures created by chromosome-organizing proteins is provided by high-resolution fluorescence microscopy techniques. http://phmd.pl/gicid/01.3001.0010.6696bacterial chromosomenucleoid associated proteins (NAP)in vivo footprintingChIP-seq3C4C |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joanna Hołówka Małgorzata Płachetka |
spellingShingle |
Joanna Hołówka Małgorzata Płachetka Structure of bacterial chromosome: An analysis of DNA-protein interactions in vivo Postępy Higieny i Medycyny Doświadczalnej bacterial chromosome nucleoid associated proteins (NAP) in vivo footprinting ChIP-seq 3C 4C |
author_facet |
Joanna Hołówka Małgorzata Płachetka |
author_sort |
Joanna Hołówka |
title |
Structure of bacterial chromosome: An analysis of DNA-protein interactions in vivo |
title_short |
Structure of bacterial chromosome: An analysis of DNA-protein interactions in vivo |
title_full |
Structure of bacterial chromosome: An analysis of DNA-protein interactions in vivo |
title_fullStr |
Structure of bacterial chromosome: An analysis of DNA-protein interactions in vivo |
title_full_unstemmed |
Structure of bacterial chromosome: An analysis of DNA-protein interactions in vivo |
title_sort |
structure of bacterial chromosome: an analysis of dna-protein interactions in vivo |
publisher |
Index Copernicus International S.A. |
series |
Postępy Higieny i Medycyny Doświadczalnej |
issn |
0032-5449 1732-2693 |
publishDate |
2017-12-01 |
description |
According to recent reports, bacterial chromosomes exhibit a hierarchical organization. The number of proteins that bind DNA are responsible for local and global organization of the DNA ensuring proper chromosome compaction. Advanced molecular biology techniques combined with high-throughput DNA sequencing methods allow a precise analysis of bacterial chromosome structures on a local and global scale. Methods such as in vivo footprinting and ChIP-seq allow to map binding sites of analyzed proteins in certain chromosomal regions or along the whole chromosome while analysis of the spatial interactions on global scale could be performed by 3C techniques. Additional insight into complex structures created by chromosome-organizing proteins is provided by high-resolution fluorescence microscopy techniques.
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topic |
bacterial chromosome nucleoid associated proteins (NAP) in vivo footprinting ChIP-seq 3C 4C |
url |
http://phmd.pl/gicid/01.3001.0010.6696 |
work_keys_str_mv |
AT joannahołowka structureofbacterialchromosomeananalysisofdnaproteininteractionsinvivo AT małgorzatapłachetka structureofbacterialchromosomeananalysisofdnaproteininteractionsinvivo |
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1725508545764392960 |