The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis
Mycoplasma bovis is associated with several clinical syndromes of cattle. Currently, limited information is available on the sensitivity (Se) and specificity (Sp) of serological assays used for the detection of M. bovis-specific antibodies. Consequently, it is difficult to critically evaluate the ou...
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doaj-b04fc29852504489a69458ad5d2240572021-04-02T03:33:11ZengMDPI AGVeterinary Sciences2306-73812018-03-01512710.3390/vetsci5010027vetsci5010027The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovisMeghan L. Schibrowski0Tamsin S. Barnes1Nadeeka K. Wawegama2Megan E. Vance3Philip F. Markham4Peter D. Mansell5Marc S. Marenda6Anna Kanci7José Perez-Casal8Glenn F. Browning9Justine S. Gibson10Timothy J. Mahony11Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, AustraliaQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, AustraliaAsia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, AustraliaAsia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaAsia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaAsia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaAsia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaVaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac), 120 Veterinary Road, Saskatoon, SK S7N 5E3, CanadaAsia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, AustraliaMycoplasma bovis is associated with several clinical syndromes of cattle. Currently, limited information is available on the sensitivity (Se) and specificity (Sp) of serological assays used for the detection of M. bovis-specific antibodies. Consequently, it is difficult to critically evaluate the outcomes of studies that use these assays. Therefore, the current study used bovine sera sourced from M. bovis exposure studies from three countries to estimate the Se and Sp of two commercial M. bovis enzyme-linked immunosorbent assays (ELISA), BIO K302 and BIO K260, and Western blotting. Western blotting had the highest Se estimate of 74% (95% confidence interval (CI): 16–98%), compared to the BIO K302: 47% (95% CI: 10–87%) and BIO K260: 28% (95% CI: 1–92%). However, for Sp, the BIO K302: 96% (95% CI: 87–99%) and the BIO K260: 100% (95% CI: 93–100%) out-performed Western blotting: 88% (95% CI: 56–98%). Western blotting was the best assay for detecting seroconversion, correctly identifying 61% (95% CI: 29–86%) of exposed animals compared to 35% for BIO K302 (95% CI: 21–54%) and 8% for BIO K260 (95% CI: 0–87%). While none of the methods assessed had high Se and Sp, the availability of these estimates will aid in the interpretation of studies that use these assays. The results of this study highlight the difficulties encountered when using serology to detect exposure to M. bovis in cattle.http://www.mdpi.com/2306-7381/5/1/27Mycoplasma bovisserologysensitivityspecificityELISAWestern blotting |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meghan L. Schibrowski Tamsin S. Barnes Nadeeka K. Wawegama Megan E. Vance Philip F. Markham Peter D. Mansell Marc S. Marenda Anna Kanci José Perez-Casal Glenn F. Browning Justine S. Gibson Timothy J. Mahony |
spellingShingle |
Meghan L. Schibrowski Tamsin S. Barnes Nadeeka K. Wawegama Megan E. Vance Philip F. Markham Peter D. Mansell Marc S. Marenda Anna Kanci José Perez-Casal Glenn F. Browning Justine S. Gibson Timothy J. Mahony The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis Veterinary Sciences Mycoplasma bovis serology sensitivity specificity ELISA Western blotting |
author_facet |
Meghan L. Schibrowski Tamsin S. Barnes Nadeeka K. Wawegama Megan E. Vance Philip F. Markham Peter D. Mansell Marc S. Marenda Anna Kanci José Perez-Casal Glenn F. Browning Justine S. Gibson Timothy J. Mahony |
author_sort |
Meghan L. Schibrowski |
title |
The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis |
title_short |
The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis |
title_full |
The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis |
title_fullStr |
The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis |
title_full_unstemmed |
The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis |
title_sort |
performance of three immune assays to assess the serological status of cattle experimentally exposed to mycoplasma bovis |
publisher |
MDPI AG |
series |
Veterinary Sciences |
issn |
2306-7381 |
publishDate |
2018-03-01 |
description |
Mycoplasma bovis is associated with several clinical syndromes of cattle. Currently, limited information is available on the sensitivity (Se) and specificity (Sp) of serological assays used for the detection of M. bovis-specific antibodies. Consequently, it is difficult to critically evaluate the outcomes of studies that use these assays. Therefore, the current study used bovine sera sourced from M. bovis exposure studies from three countries to estimate the Se and Sp of two commercial M. bovis enzyme-linked immunosorbent assays (ELISA), BIO K302 and BIO K260, and Western blotting. Western blotting had the highest Se estimate of 74% (95% confidence interval (CI): 16–98%), compared to the BIO K302: 47% (95% CI: 10–87%) and BIO K260: 28% (95% CI: 1–92%). However, for Sp, the BIO K302: 96% (95% CI: 87–99%) and the BIO K260: 100% (95% CI: 93–100%) out-performed Western blotting: 88% (95% CI: 56–98%). Western blotting was the best assay for detecting seroconversion, correctly identifying 61% (95% CI: 29–86%) of exposed animals compared to 35% for BIO K302 (95% CI: 21–54%) and 8% for BIO K260 (95% CI: 0–87%). While none of the methods assessed had high Se and Sp, the availability of these estimates will aid in the interpretation of studies that use these assays. The results of this study highlight the difficulties encountered when using serology to detect exposure to M. bovis in cattle. |
topic |
Mycoplasma bovis serology sensitivity specificity ELISA Western blotting |
url |
http://www.mdpi.com/2306-7381/5/1/27 |
work_keys_str_mv |
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