Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys
Non-invasive tests to identify age and early disease-associated pathology within the kidney are needed. Specific populations of urinary extracellular vesicles (EVs) could potentially be used for such a diagnostic test. Random urine samples were obtained from age- and sex-stratified living kidney don...
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doaj-b057152a2b1b418d8e1421b683d85c1e2020-11-24T23:30:48ZengTaylor & Francis GroupJournal of Extracellular Vesicles2001-30782016-02-015011210.3402/jev.v5.2964229642Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneysAnne E. Turco0Wing Lam1Andrew D. Rule2Aleksandar Denic3John C. Lieske4Virginia M. Miller5Joseph J. Larson6Walter K. Kremers7Muthuvel Jayachandran8 Department of Physiology & Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, MN, USA Department of Physiology & Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, MN, USA Division of Nephrology and Hypertension, Department of Internal Medicine, College of Medicine, Mayo Clinic, Rochester, MN, USA Division of Nephrology and Hypertension, Department of Internal Medicine, College of Medicine, Mayo Clinic, Rochester, MN, USA Division of Nephrology and Hypertension, Department of Internal Medicine, College of Medicine, Mayo Clinic, Rochester, MN, USA Department of Physiology & Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, MN, USA Department of Health Sciences Research, College of Medicine, Mayo Clinic, Rochester, MN, USA Department of Health Sciences Research, College of Medicine, Mayo Clinic, Rochester, MN, USA Department of Physiology & Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, MN, USANon-invasive tests to identify age and early disease-associated pathology within the kidney are needed. Specific populations of urinary extracellular vesicles (EVs) could potentially be used for such a diagnostic test. Random urine samples were obtained from age- and sex-stratified living kidney donors before kidney donation. A biopsy of the donor kidney was obtained at the time of transplantation to identify nephron hypertrophy (larger glomerular volume, cortex per glomerulus and mean profile tubular area) and nephrosclerosis (% fibrosis, % glomerulosclerosis and arteriosclerosis). Renal parenchymal-derived EVs in cell-free urine were quantified by digital flow cytometry. The relationship between these EV populations and structural pathology on the kidney biopsy was assessed. Clinical characteristics of the kidney donors (n=138, age range: 20–70 years, 50% women) were within the normative range. Overall, urine from women contained more EVs than that from men. The number of exosomes, juxtaglomerular cells and podocyte marker–positive EVs decreased (p<0.05) with increasing age. There were fewer total EVs as well as EVs positive for mesangial cell, parietal cell, descending limb of Henle's loop (simple squamous epithelium), collecting tubule-intercalated cell and monocyte chemoattractant protein-1 markers (p<0.05) in persons with nephron hypertrophy. The number of EVs positive for intercellular adhesion molecule-1, juxtaglomerular cell, podocyte, parietal cell, proximal tubular epithelial cell, distal tubular epithelial cell and collecting duct cells were fewer (p<0.05) in persons with nephrosclerosis. EVs carrying markers of cells from the renal pelvis epithelium did not associate with any indices of nephron hypertrophy or nephrosclerosis. Therefore, specific populations of EVs derived from cells of the glomerulus and nephron associate with underlying kidney structural changes. Further validation of these findings in other cohorts is needed to determine their clinical utility.http://www.journalofextracellularvesicles.net/index.php/jev/article/view/29642/pdf_50microvesiclesmicroparticlesexosomesurinary vesiclesfibrosisnephron hypertrophyglomerulosclerosisarteriosclerosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anne E. Turco Wing Lam Andrew D. Rule Aleksandar Denic John C. Lieske Virginia M. Miller Joseph J. Larson Walter K. Kremers Muthuvel Jayachandran |
spellingShingle |
Anne E. Turco Wing Lam Andrew D. Rule Aleksandar Denic John C. Lieske Virginia M. Miller Joseph J. Larson Walter K. Kremers Muthuvel Jayachandran Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys Journal of Extracellular Vesicles microvesicles microparticles exosomes urinary vesicles fibrosis nephron hypertrophy glomerulosclerosis arteriosclerosis |
author_facet |
Anne E. Turco Wing Lam Andrew D. Rule Aleksandar Denic John C. Lieske Virginia M. Miller Joseph J. Larson Walter K. Kremers Muthuvel Jayachandran |
author_sort |
Anne E. Turco |
title |
Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys |
title_short |
Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys |
title_full |
Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys |
title_fullStr |
Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys |
title_full_unstemmed |
Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys |
title_sort |
specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys |
publisher |
Taylor & Francis Group |
series |
Journal of Extracellular Vesicles |
issn |
2001-3078 |
publishDate |
2016-02-01 |
description |
Non-invasive tests to identify age and early disease-associated pathology within the kidney are needed. Specific populations of urinary extracellular vesicles (EVs) could potentially be used for such a diagnostic test. Random urine samples were obtained from age- and sex-stratified living kidney donors before kidney donation. A biopsy of the donor kidney was obtained at the time of transplantation to identify nephron hypertrophy (larger glomerular volume, cortex per glomerulus and mean profile tubular area) and nephrosclerosis (% fibrosis, % glomerulosclerosis and arteriosclerosis). Renal parenchymal-derived EVs in cell-free urine were quantified by digital flow cytometry. The relationship between these EV populations and structural pathology on the kidney biopsy was assessed. Clinical characteristics of the kidney donors (n=138, age range: 20–70 years, 50% women) were within the normative range. Overall, urine from women contained more EVs than that from men. The number of exosomes, juxtaglomerular cells and podocyte marker–positive EVs decreased (p<0.05) with increasing age. There were fewer total EVs as well as EVs positive for mesangial cell, parietal cell, descending limb of Henle's loop (simple squamous epithelium), collecting tubule-intercalated cell and monocyte chemoattractant protein-1 markers (p<0.05) in persons with nephron hypertrophy. The number of EVs positive for intercellular adhesion molecule-1, juxtaglomerular cell, podocyte, parietal cell, proximal tubular epithelial cell, distal tubular epithelial cell and collecting duct cells were fewer (p<0.05) in persons with nephrosclerosis. EVs carrying markers of cells from the renal pelvis epithelium did not associate with any indices of nephron hypertrophy or nephrosclerosis. Therefore, specific populations of EVs derived from cells of the glomerulus and nephron associate with underlying kidney structural changes. Further validation of these findings in other cohorts is needed to determine their clinical utility. |
topic |
microvesicles microparticles exosomes urinary vesicles fibrosis nephron hypertrophy glomerulosclerosis arteriosclerosis |
url |
http://www.journalofextracellularvesicles.net/index.php/jev/article/view/29642/pdf_50 |
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