Accelerating dermal wound healing and mitigating excessive scar formation using LBL modified nanofibrous mats
Developing an ideal wound dressing material which could accelerate wound closure and achieve scarless wound healing was the ultimate goal of numerous researchers. In this study, biomimetic silk fibroin (SF)/polycaprolactone (PCL) matrices were fabricated via co-electrospinning and positively charged...
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doaj-b0571bf43e304ee6ba2d8e8eeb8d38c32020-11-25T01:42:16ZengElsevierMaterials & Design0264-12752020-01-01185Accelerating dermal wound healing and mitigating excessive scar formation using LBL modified nanofibrous matsGuomin Wu0Xiao Ma1Le Fan2Yuying Gao3Hongbing Deng4Yining Wang5The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China; Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan, 430079, ChinaGuangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, ChinaHubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan, 430079, ChinaHubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan, 430079, China; Corresponding author.The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China; Corresponding author.Developing an ideal wound dressing material which could accelerate wound closure and achieve scarless wound healing was the ultimate goal of numerous researchers. In this study, biomimetic silk fibroin (SF)/polycaprolactone (PCL) matrices were fabricated via co-electrospinning and positively charged chitosan (CS) and negatively charged type I collagen (COL) were deposited on the nanofibrous mats through electrostatic layer-by-layer (LBL) self-assembly technique. Scanning electron microscopy images indicate that the average fiber diameter of SF/PCL nanofibers became larger and more and more irregular protuberances were observed on their surfaces with the LBL process. Besides, the chemical structure and composition investigation further verified the successful deposition of CS/COL. Additionally, tensile strength and water contact angle tests showed the LBL modified mats had enhanced mechanical properties and good hydrophilicity. Moreover, LBL structured mats acquired excellent antibacterial activity and better ability to promote cell attachment, growth and proliferation. Ultimately, in vivo wound healing assay in rat models revealed that LBL structured mats could reduce the wound closure time, increase collagen production and mitigate excessive scar formation through TGF-β/Smad signaling pathways, which demonstrated the potential application of the nanofibrous mats in skin regeneration. Keywords: Silk fibroin, Chitosan, Collagen, Nanofibrous mats, Wound healinghttp://www.sciencedirect.com/science/article/pii/S0264127519307038 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guomin Wu Xiao Ma Le Fan Yuying Gao Hongbing Deng Yining Wang |
spellingShingle |
Guomin Wu Xiao Ma Le Fan Yuying Gao Hongbing Deng Yining Wang Accelerating dermal wound healing and mitigating excessive scar formation using LBL modified nanofibrous mats Materials & Design |
author_facet |
Guomin Wu Xiao Ma Le Fan Yuying Gao Hongbing Deng Yining Wang |
author_sort |
Guomin Wu |
title |
Accelerating dermal wound healing and mitigating excessive scar formation using LBL modified nanofibrous mats |
title_short |
Accelerating dermal wound healing and mitigating excessive scar formation using LBL modified nanofibrous mats |
title_full |
Accelerating dermal wound healing and mitigating excessive scar formation using LBL modified nanofibrous mats |
title_fullStr |
Accelerating dermal wound healing and mitigating excessive scar formation using LBL modified nanofibrous mats |
title_full_unstemmed |
Accelerating dermal wound healing and mitigating excessive scar formation using LBL modified nanofibrous mats |
title_sort |
accelerating dermal wound healing and mitigating excessive scar formation using lbl modified nanofibrous mats |
publisher |
Elsevier |
series |
Materials & Design |
issn |
0264-1275 |
publishDate |
2020-01-01 |
description |
Developing an ideal wound dressing material which could accelerate wound closure and achieve scarless wound healing was the ultimate goal of numerous researchers. In this study, biomimetic silk fibroin (SF)/polycaprolactone (PCL) matrices were fabricated via co-electrospinning and positively charged chitosan (CS) and negatively charged type I collagen (COL) were deposited on the nanofibrous mats through electrostatic layer-by-layer (LBL) self-assembly technique. Scanning electron microscopy images indicate that the average fiber diameter of SF/PCL nanofibers became larger and more and more irregular protuberances were observed on their surfaces with the LBL process. Besides, the chemical structure and composition investigation further verified the successful deposition of CS/COL. Additionally, tensile strength and water contact angle tests showed the LBL modified mats had enhanced mechanical properties and good hydrophilicity. Moreover, LBL structured mats acquired excellent antibacterial activity and better ability to promote cell attachment, growth and proliferation. Ultimately, in vivo wound healing assay in rat models revealed that LBL structured mats could reduce the wound closure time, increase collagen production and mitigate excessive scar formation through TGF-β/Smad signaling pathways, which demonstrated the potential application of the nanofibrous mats in skin regeneration. Keywords: Silk fibroin, Chitosan, Collagen, Nanofibrous mats, Wound healing |
url |
http://www.sciencedirect.com/science/article/pii/S0264127519307038 |
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