Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode <i>Bursaphelenchus xylophilus</i>
Pine wilt disease (PWD) caused by pine wood nematode (PWN), <i>Bursaphelenchus xylophilus</i>, is a serious threat to global forest populations of conifers, in particular <i>Pinus spp</i>. Recently, the presence of PWN was reported in dead Yunnan pine (<i>Pinus yunnanen...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-02-01
|
Series: | Forests |
Subjects: | |
Online Access: | https://www.mdpi.com/1999-4907/11/2/204 |
id |
doaj-b05bfbe76671475590a60d98b8a3d51a |
---|---|
record_format |
Article |
spelling |
doaj-b05bfbe76671475590a60d98b8a3d51a2020-11-25T01:42:25ZengMDPI AGForests1999-49072020-02-0111220410.3390/f11020204f11020204Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode <i>Bursaphelenchus xylophilus</i>Daniel Gaspar0Cândida Trindade1Ana Usié2Brigida Meireles3Ana Margarida Fortes4Joana Bagoin Guimarães5Fernanda Simões6Rita Lourenço Costa7António Marcos Ramos8Centro de Biotecnologia Agrícola e Agro-alimentar do Alentejo (CEBAL)/Instituto Politécnico de Beja (IPBeja), 7801-908 Beja, PortugalINIAV—Instituto Nacional de Investigação Agrária e Veterinária, 2780-157 Oeiras, PortugalCentro de Biotecnologia Agrícola e Agro-alimentar do Alentejo (CEBAL)/Instituto Politécnico de Beja (IPBeja), 7801-908 Beja, PortugalCentro de Biotecnologia Agrícola e Agro-alimentar do Alentejo (CEBAL)/Instituto Politécnico de Beja (IPBeja), 7801-908 Beja, PortugalFaculdade de Ciências de Lisboa, BioISI, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, PortugalINIAV—Instituto Nacional de Investigação Agrária e Veterinária, 2780-157 Oeiras, PortugalINIAV—Instituto Nacional de Investigação Agrária e Veterinária, 2780-157 Oeiras, PortugalINIAV—Instituto Nacional de Investigação Agrária e Veterinária, 2780-157 Oeiras, PortugalCentro de Biotecnologia Agrícola e Agro-alimentar do Alentejo (CEBAL)/Instituto Politécnico de Beja (IPBeja), 7801-908 Beja, PortugalPine wilt disease (PWD) caused by pine wood nematode (PWN), <i>Bursaphelenchus xylophilus</i>, is a serious threat to global forest populations of conifers, in particular <i>Pinus spp</i>. Recently, the presence of PWN was reported in dead Yunnan pine (<i>Pinus yunnanensis</i>) trees under natural conditions. To further understand the potential impact caused by PWN in Yunnan pine populations, a transcriptional profiling analysis was performed over different time points (0 hours (h), 6 h, 24 h, 48 h, and 7 days) after PWN inoculation. A total of 9961 differentially expressed genes were identified after inoculation, which suggested a dynamic response against the pathogen, with a more intense pattern at 48 h after inoculation. The results also highlighted a set of biological mechanisms triggered after inoculation that provide valuable information regarding the response of Yunnan pine to PWN infection. When compared with maritime pine (<i>Pinus pinaster</i>), the Yunnan pine response was less complex and involved a smaller number of differentially expressed genes, which may be associated with the increased degree of resistance to PWN displayed by Yunnan pine. These results revealed different strategies to cope with PWN infection by these two pine species, which display contrasting degrees of susceptibility, especially in the timely perception of the infection and response magnitude.https://www.mdpi.com/1999-4907/11/2/204rna-sequencingtranscriptome analysisgene expressionpine wilt disease<i>pinus pinaster</i><i>bursaphelenchus xylophilus</i><i>pinus yunnanensis</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniel Gaspar Cândida Trindade Ana Usié Brigida Meireles Ana Margarida Fortes Joana Bagoin Guimarães Fernanda Simões Rita Lourenço Costa António Marcos Ramos |
spellingShingle |
Daniel Gaspar Cândida Trindade Ana Usié Brigida Meireles Ana Margarida Fortes Joana Bagoin Guimarães Fernanda Simões Rita Lourenço Costa António Marcos Ramos Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode <i>Bursaphelenchus xylophilus</i> Forests rna-sequencing transcriptome analysis gene expression pine wilt disease <i>pinus pinaster</i> <i>bursaphelenchus xylophilus</i> <i>pinus yunnanensis</i> |
author_facet |
Daniel Gaspar Cândida Trindade Ana Usié Brigida Meireles Ana Margarida Fortes Joana Bagoin Guimarães Fernanda Simões Rita Lourenço Costa António Marcos Ramos |
author_sort |
Daniel Gaspar |
title |
Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode <i>Bursaphelenchus xylophilus</i> |
title_short |
Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode <i>Bursaphelenchus xylophilus</i> |
title_full |
Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode <i>Bursaphelenchus xylophilus</i> |
title_fullStr |
Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode <i>Bursaphelenchus xylophilus</i> |
title_full_unstemmed |
Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode <i>Bursaphelenchus xylophilus</i> |
title_sort |
comparative transcriptomic response of two pinus species to infection with the pine wood nematode <i>bursaphelenchus xylophilus</i> |
publisher |
MDPI AG |
series |
Forests |
issn |
1999-4907 |
publishDate |
2020-02-01 |
description |
Pine wilt disease (PWD) caused by pine wood nematode (PWN), <i>Bursaphelenchus xylophilus</i>, is a serious threat to global forest populations of conifers, in particular <i>Pinus spp</i>. Recently, the presence of PWN was reported in dead Yunnan pine (<i>Pinus yunnanensis</i>) trees under natural conditions. To further understand the potential impact caused by PWN in Yunnan pine populations, a transcriptional profiling analysis was performed over different time points (0 hours (h), 6 h, 24 h, 48 h, and 7 days) after PWN inoculation. A total of 9961 differentially expressed genes were identified after inoculation, which suggested a dynamic response against the pathogen, with a more intense pattern at 48 h after inoculation. The results also highlighted a set of biological mechanisms triggered after inoculation that provide valuable information regarding the response of Yunnan pine to PWN infection. When compared with maritime pine (<i>Pinus pinaster</i>), the Yunnan pine response was less complex and involved a smaller number of differentially expressed genes, which may be associated with the increased degree of resistance to PWN displayed by Yunnan pine. These results revealed different strategies to cope with PWN infection by these two pine species, which display contrasting degrees of susceptibility, especially in the timely perception of the infection and response magnitude. |
topic |
rna-sequencing transcriptome analysis gene expression pine wilt disease <i>pinus pinaster</i> <i>bursaphelenchus xylophilus</i> <i>pinus yunnanensis</i> |
url |
https://www.mdpi.com/1999-4907/11/2/204 |
work_keys_str_mv |
AT danielgaspar comparativetranscriptomicresponseoftwopinusspeciestoinfectionwiththepinewoodnematodeibursaphelenchusxylophilusi AT candidatrindade comparativetranscriptomicresponseoftwopinusspeciestoinfectionwiththepinewoodnematodeibursaphelenchusxylophilusi AT anausie comparativetranscriptomicresponseoftwopinusspeciestoinfectionwiththepinewoodnematodeibursaphelenchusxylophilusi AT brigidameireles comparativetranscriptomicresponseoftwopinusspeciestoinfectionwiththepinewoodnematodeibursaphelenchusxylophilusi AT anamargaridafortes comparativetranscriptomicresponseoftwopinusspeciestoinfectionwiththepinewoodnematodeibursaphelenchusxylophilusi AT joanabagoinguimaraes comparativetranscriptomicresponseoftwopinusspeciestoinfectionwiththepinewoodnematodeibursaphelenchusxylophilusi AT fernandasimoes comparativetranscriptomicresponseoftwopinusspeciestoinfectionwiththepinewoodnematodeibursaphelenchusxylophilusi AT ritalourencocosta comparativetranscriptomicresponseoftwopinusspeciestoinfectionwiththepinewoodnematodeibursaphelenchusxylophilusi AT antoniomarcosramos comparativetranscriptomicresponseoftwopinusspeciestoinfectionwiththepinewoodnematodeibursaphelenchusxylophilusi |
_version_ |
1725036526004338688 |