Burst-Dependent Bidirectional Plasticity in the Cerebellum Is Driven by Presynaptic NMDA Receptors
Numerous studies have shown that cerebellar function is related to the plasticity at the synapses between parallel fibers and Purkinje cells. How specific input patterns determine plasticity outcomes, as well as the biophysics underlying plasticity of these synapses, remain unclear. Here, we charact...
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doaj-b08d979f9057409a97f7bc987933cf552020-11-24T21:33:42ZengElsevierCell Reports2211-12472016-04-0115110411610.1016/j.celrep.2016.03.004Burst-Dependent Bidirectional Plasticity in the Cerebellum Is Driven by Presynaptic NMDA ReceptorsGuy Bouvier0David Higgins1Maria Spolidoro2Damien Carrel3Benjamin Mathieu4Clément Léna5Stéphane Dieudonné6Boris Barbour7Nicolas Brunel8Mariano Casado9Ecole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), Inserm U1024, CNRS UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), Inserm U1024, CNRS UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), Inserm U1024, CNRS UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), Inserm U1024, CNRS UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), Inserm U1024, CNRS UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), Inserm U1024, CNRS UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), Inserm U1024, CNRS UMR 8197, Paris 75005, FranceEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), Inserm U1024, CNRS UMR 8197, Paris 75005, FranceDepartments of Statistics and Neurobiology, University of Chicago, Chicago, IL 60637, USAEcole Normale Supérieure, Institut de Biologie de l’ENS (IBENS), Inserm U1024, CNRS UMR 8197, Paris 75005, FranceNumerous studies have shown that cerebellar function is related to the plasticity at the synapses between parallel fibers and Purkinje cells. How specific input patterns determine plasticity outcomes, as well as the biophysics underlying plasticity of these synapses, remain unclear. Here, we characterize the patterns of activity that lead to postsynaptically expressed LTP using both in vivo and in vitro experiments. Similar to the requirements of LTD, we find that high-frequency bursts are necessary to trigger LTP and that this burst-dependent plasticity depends on presynaptic NMDA receptors and nitric oxide (NO) signaling. We provide direct evidence for calcium entry through presynaptic NMDA receptors in a subpopulation of parallel fiber varicosities. Finally, we develop and experimentally verify a mechanistic plasticity model based on NO and calcium signaling. The model reproduces plasticity outcomes from data and predicts the effect of arbitrary patterns of synaptic inputs on Purkinje cells, thereby providing a unified description of plasticity.http://www.sciencedirect.com/science/article/pii/S2211124716302285 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guy Bouvier David Higgins Maria Spolidoro Damien Carrel Benjamin Mathieu Clément Léna Stéphane Dieudonné Boris Barbour Nicolas Brunel Mariano Casado |
spellingShingle |
Guy Bouvier David Higgins Maria Spolidoro Damien Carrel Benjamin Mathieu Clément Léna Stéphane Dieudonné Boris Barbour Nicolas Brunel Mariano Casado Burst-Dependent Bidirectional Plasticity in the Cerebellum Is Driven by Presynaptic NMDA Receptors Cell Reports |
author_facet |
Guy Bouvier David Higgins Maria Spolidoro Damien Carrel Benjamin Mathieu Clément Léna Stéphane Dieudonné Boris Barbour Nicolas Brunel Mariano Casado |
author_sort |
Guy Bouvier |
title |
Burst-Dependent Bidirectional Plasticity in the Cerebellum Is Driven by Presynaptic NMDA Receptors |
title_short |
Burst-Dependent Bidirectional Plasticity in the Cerebellum Is Driven by Presynaptic NMDA Receptors |
title_full |
Burst-Dependent Bidirectional Plasticity in the Cerebellum Is Driven by Presynaptic NMDA Receptors |
title_fullStr |
Burst-Dependent Bidirectional Plasticity in the Cerebellum Is Driven by Presynaptic NMDA Receptors |
title_full_unstemmed |
Burst-Dependent Bidirectional Plasticity in the Cerebellum Is Driven by Presynaptic NMDA Receptors |
title_sort |
burst-dependent bidirectional plasticity in the cerebellum is driven by presynaptic nmda receptors |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2016-04-01 |
description |
Numerous studies have shown that cerebellar function is related to the plasticity at the synapses between parallel fibers and Purkinje cells. How specific input patterns determine plasticity outcomes, as well as the biophysics underlying plasticity of these synapses, remain unclear. Here, we characterize the patterns of activity that lead to postsynaptically expressed LTP using both in vivo and in vitro experiments. Similar to the requirements of LTD, we find that high-frequency bursts are necessary to trigger LTP and that this burst-dependent plasticity depends on presynaptic NMDA receptors and nitric oxide (NO) signaling. We provide direct evidence for calcium entry through presynaptic NMDA receptors in a subpopulation of parallel fiber varicosities. Finally, we develop and experimentally verify a mechanistic plasticity model based on NO and calcium signaling. The model reproduces plasticity outcomes from data and predicts the effect of arbitrary patterns of synaptic inputs on Purkinje cells, thereby providing a unified description of plasticity. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124716302285 |
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